Actin Cytoskeletal Lesions in Differentiated Human Colon Carcinoma Caoo-2 Cells After Exposure to Soybean Agglutinin

J. Draaijer; J. Koninkx; H. Hendriks; M. Kik; J. van Dijk; J. Mouwen

Actin Cytoskeletal Lesions in Differentiated Human Colon Carcinoma Caoo-2 Cells After Exposure to Soybean Agglutinin

In: Recent advances of research in antinutritional factors in legume seeds

Authors:

J. Draaijer

J. Draaijer Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, P.O. Box 80.158, 3508 TD Utrecht, The Netherlands

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J. Koninkx

J. Koninkx Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, P.O. Box 80.158, 3508 TD Utrecht, The Netherlands

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H. Hendriks

H. Hendriks Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, P.O. Box 80.158, 3508 TD Utrecht, The Netherlands

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M. Kik

M. Kik Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, P.O. Box 80.158, 3508 TD Utrecht, The Netherlands

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J. van Dijk

J. van Dijk Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, P.O. Box 80.158, 3508 TD Utrecht, The Netherlands

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J. Mouwen

J. Mouwen Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Utrecht, Yalelaan 1, P.O. Box 80.158, 3508 TD Utrecht, The Netherlands

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Type:: Chapter

Pages:: 59–62

DOI:: https://doi.org/10.1163/9789004684690_012

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Summary

We have investigated the effects of soybean agglutinin on the cytoskeletal element actin in differentiated Caco-2 cells. The actin cytoskeleton of the cells was visualized by fluorescence microscopy using NBD-phallacidin as a specific marker for F-actin. Using the deoxyribonuclease-I inhibition assay depolymerization of F-actin could be established after exposure for 15 min. to soybean agglutinin (SBA). In addition, an increase of intracellular G-actin was measured. The induction of depolymerization could be prevented by incubating the Caco-2 cells with SBA and N-acetylgalactosamine simultaneously. The increase in the amount of G-actin appeared to be correlated with a shortening of microvilli of the Caco-2 cells.

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Recent advances of research in antinutritional factors in legume seeds

Proceedings of the first international workshop on ANF in legume seeds, Wageningen 23-25 nov. '88

E-Book ISBN:: 9789004684690

Publisher:: Wageningen Academic

Print Publication Date:: 01 Jan 1989

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- Life Sciences

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Actin Cytoskeletal Lesions in Differentiated Human Colon Carcinoma Caoo-2 Cells After Exposure to Soybean Agglutinin

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Citation Info

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Recent advances of research in antinutritional factors in legume seeds

Proceedings of the first international workshop on ANF in legume seeds, Wageningen 23-25 nov. '88

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