Sequencing of the mink genome: plans and perspectives

R. Anistoroaei; K. Christensen; B. Benkel

Sequencing of the mink genome: plans and perspectives

In: Proceedings of the Xth International Scientific Congress in Fur Animal Production

Authors:

R. Anistoroaei

R. Anistoroaei ¹University of Copenhagen, Faculty of Health Sciences, Dept. of Animal and Veterinary Basic Sciences, Division of Animal Genetics and Bioinformatics, Groennegaardsvej 3, 1870 Frederiksberg C,Denmark

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K. Christensen

K. Christensen ¹University of Copenhagen, Faculty of Health Sciences, Dept. of Animal and Veterinary Basic Sciences, Division of Animal Genetics and Bioinformatics, Groennegaardsvej 3, 1870 Frederiksberg C,Denmark

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, and

B. Benkel

B. Benkel ²Nova Scotia Agricultural College, Department of Plant and Animal Sciences, P.O. Box 550,Truro, B2N 5E3 Nova Scotia, Canada; ran@life.ku.dk

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Type:: Chapter

Pages:: 207–215

DOI:: https://doi.org/10.3920/9789086867608_033

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Dar Hadith al Hassania

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The assembled sequence of the whole genome is a must nowadays in any industrially important species. The aim of this research program is to provide the industry and the community with a robust mink genome sequence assembly. It shall be complemented with the identification of widely distributed polymorphic single nucleotide polymorphisms (SNPs) organized into an array designed for undertaking linkage and association studies necessary for mapping, identifying and characterizing genes underlying any specific traits of interest in American mink. Genome assembly based on the sequences of individual clones from the mink Bacterial Artificial Chromosome (BAC) library would result in a draft genome of high quality and accuracy. We explore an alternative approach, called BAC-NGS (from next generation sequencing) that utilizes the BAC library as a solid backbone, complemented with shotgun sequences of the whole genome, for filling gaps for the final assembly. We tested different settings of the new approach in several pilot projects in order to assign the most efficacious approach to sequence assembly. Approximately 15% of the mink genome has so far been generated and assembled by means of this method. This sequencing project represents a long-term investment in the future mink fur industry and farming management, enhancing the development of applications in academic and industrial research.

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Proceedings of the Xth International Scientific Congress in Fur Animal Production

Scientifur Volume 36 (3/4)

E-Book ISBN:: 9789086867608

Publisher:: Wageningen Academic

Print Publication Date:: 30 Oct 2012

Subjects
- Life Sciences
  - General
  - Animal & Veterinary

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Sequencing of the mink genome: plans and perspectives

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Proceedings of the Xth International Scientific Congress in Fur Animal Production

Scientifur Volume 36 (3/4)

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