Validation of the 13C-bicarbonate tracer technique for estimation of CO2 production and energy expenditure in mink (Neovison vison)

C. Larsson; A.-H. Tauson

Validation of the ¹³C-bicarbonate tracer technique for estimation of CO₂ production and energy expenditure in mink (Neovison vison)

In: Proceedings of the Xth International Scientific Congress in Fur Animal Production

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C. Larsson

C. Larsson Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 3, 1870 Frederiksberg C, Denmark; aht@life.ku.dk

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A.-H. Tauson

A.-H. Tauson Faculty of Health and Medical Sciences, University of Copenhagen, Grønnegårdsvej 3, 1870 Frederiksberg C, Denmark; aht@life.ku.dk

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Type:: Chapter

Pages:: 54–62

DOI:: https://doi.org/10.3920/9789086867608_008

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Dar Hadith al Hassania

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The objective was to validate the ¹³C-bicarbonate tracer technique (¹³CT) for estimation of the CO₂ production (RCO₂) and energy expenditure (EE) against ‘the golden standard’ indirect calorimetry (IC), in mink in the fed state. Six male mink were measured in a Latin-square design, simultaneously by means of ¹³CT and IC. A dose of NaH¹³CO₃ was administrated orally, by subcutaneous or intra muscular injection. A zero breath sample was collected before the isotope administration. After dose administration breath samples were collected at frequent intervals for 6 h, and simultaneously the animals were measured by IC for 22 h. There was excellent agreement between results from ¹³CT and IC, and when using the measured factor for recovery of ¹³C in breath and measured RQ values, no significant differences between administration methods in estimates of RCO₂ and EE were found. The ¹³CT was concluded to be a useful method for estimating EE, regardless of administration method, but accuracy is dependent on the estimates used for RF and RQ.

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Proceedings of the Xth International Scientific Congress in Fur Animal Production

Scientifur Volume 36 (3/4)

E-Book ISBN:: 9789086867608

Publisher:: Wageningen Academic

Print Publication Date:: 30 Oct 2012

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- Life Sciences
  - General
  - Animal & Veterinary

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Validation of the 13C-bicarbonate tracer technique for estimation of CO2 production and energy expenditure in mink (Neovison vison)

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Proceedings of the Xth International Scientific Congress in Fur Animal Production

Scientifur Volume 36 (3/4)

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Validation of the ¹³C-bicarbonate tracer technique for estimation of CO₂ production and energy expenditure in mink (Neovison vison)