Multimycotoxin analysis: The LC-MS approach

M.C. Spanjer; P.M. Rensen; J.M. Scholten

Multimycotoxin analysis: The LC-MS approach

In: The mycotoxin factbook

Authors:

M.C. Spanjer

M.C. Spanjer Food and Consumer Product Safety Authority, Amsterdam, The Netherlands

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P.M. Rensen

P.M. Rensen Food and Consumer Product Safety Authority, Amsterdam, The Netherlands

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J.M. Scholten

J.M. Scholten Food and Consumer Product Safety Authority, Amsterdam, The Netherlands

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Type:: Chapter

Pages:: 249–267

DOI:: https://doi.org/10.3920/9789086865871_015

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Mycotoxin analysis is mainly carried out by high performance liquid chromatography after immunoassay clean-up or in enzyme-linked immunosorbent assay tests. These methods imply determination of single compounds. To maintain legal limits it would be preferable to determine mycotoxins by routine analysis in different types of matrices in one single extract. This also holds for HACCP control purposes. Application of liquid chromatography-mass spectrometry made it possible to obtain such a method. Liquid chromatography separation of the mycotoxins is done with a gradient of water and acetonitrile on a reversed-phase column within 25 minutes. The mass spectrometer is used with an electrospray ionisation interface in the positive mode. By multiple reaction monitoring it is possible to analyse fragment ions, to increase specificity for necessary quality control. Thus far, this multimycotoxin method has been developed to include aflatoxins B₁, B₂, G₁ and G₂, ochratoxin A, deoxynivalenol, nivalenol, fusarenon-X, 3 and 15-acetyl-deoxynivalenol, fumonisins B₁, B₂ and B₃, diacetoxyscirpenol, zearalenone and derivatives, T-2 and HT-2 toxins, roquefortin, citrinin, cyclopiazonic acid, ergotamine, penicillic acid and sterigmatocystin. When the method was applied to food samples, i.e. peanut and cornflakes, a matrix effect showed up, which was expected due to the lack of clean-up. The matrix effect depends on the sample type. Validation data are presented for aflatoxins, ochratoxin A and deoxynivalenol in peanuts and cornflakes. The advantage of the multimycotoxin method has been proven by findings of more than one mycotoxin in maize, buckwheat, fig and pistachio samples. Recent developments are the application of the multimycotoxin method for baby food, which is subject to lower limits for aflatoxin B1 and ochratoxin A, and for ergot alkaloids.

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The mycotoxin factbook

E-Book ISBN:: 9789086865871

Publisher:: Wageningen Academic

Print Publication Date:: 10 Oct 2006

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- Life Sciences

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Multimycotoxin analysis: The LC-MS approach

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The mycotoxin factbook

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