Potential association of polymorphisms in the bovine CXCR2 gene with neutrophil survival and killing ability

Gina M. Pighetti; Magdalena Rambeaud

Potential association of polymorphisms in the bovine CXCR2 gene with neutrophil survival and killing ability

In: Mastitis in dairy production

Authors:

Gina M. Pighetti

Gina M. Pighetti Department of Animal Science, The University of Tennessee, Knoxville, USA

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Magdalena Rambeaud

Magdalena Rambeaud Department of Animal Science, The University of Tennessee, Knoxville, USA

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Type:: Chapter

Pages:: 185–189

DOI:: https://doi.org/10.3920/9789086865505_024

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Recent research in our lab has demonstrated a significant association between the incidence of subclinical mastitis and specific polymorphisms of the CXCR2 gene in Holstein dairy cows. This gene encodes the interleukin-8 (IL-8) receptor, a key regulator of neutrophil migration, killing and survival. Because of the importance of this gene in neutrophil regulation, we hypothesized that differences in neutrophil killing and survival may exist among the CXCR2 genotypes and potentially contribute to the observed variation in intramammary infections. To test this hypothesis, neutrophils were isolated from cows representing each CXCR2+777 genotype (GG, GC, CC; n = 10 or 20 each) and tested for reactive oxygen species (ROS) generation, bactericidal activity, and suppression of apoptosis. A significant reduction in neutrophil ROS generation in response to phorbol-13-myristate- 12 acetate (PMA) was observed in cows with a CC genotype when compared to those with a GG genotype. However, no differences in bactericidal activity were observed. In contrast to the observed ROS and bactericidal activities, a significant increase in survival was observed for neutrophils from cows with CC genotype when compared to those with a GG genotype in response to IL-8, but not dexamethasone. The functional activity of neutrophils from cows heterozygous for this polymorphism was intermediate between those with homozygous genotypes. In summary, our preliminary results indicate that neutrophils from Holstein cows with different CXCR2 genotypes vary in their ability to produce ROS and suppress apoptosis. These differences have the potential to influence overall neutrophil function and may partially explain the variation observed with respect to mastitis in vivo. These results provide a solid foundation for future research aimed at better understanding the basic differences between dairy cows genetically more or less susceptible to mastitis and has the potential to provide novel preventive and therapeutic measures against inflammatory diseases such as mastitis.

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Mastitis in dairy production

Current knowledge and future solutions

E-Book ISBN:: 9789086865505

Publisher:: Wageningen Academic

Print Publication Date:: 24 Oct 2005

Subjects
- Life Sciences

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Potential association of polymorphisms in the bovine CXCR2 gene with neutrophil survival and killing ability

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Mastitis in dairy production

Current knowledge and future solutions

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