Changes in leucocyte surface receptor expression and functional responses in dairy cows with Staphylococcus aureus infection

H. Nagahata; H. Higuchi; M. Nakamura; K. Yayou; K. Kawai; A. Ogata

Changes in leucocyte surface receptor expression and functional responses in dairy cows with Staphylococcus aureus infection

In: Mastitis in dairy production

Authors:

H. Nagahata

H. Nagahata ¹Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan

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H. Higuchi

H. Higuchi ¹Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan

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M. Nakamura

M. Nakamura ²Hokkaido National Agricultural Research Center, Sapporo, Japan

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K. Yayou

K. Yayou ²Hokkaido National Agricultural Research Center, Sapporo, Japan

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K. Kawai

K. Kawai ³Veterinary Clinical Center, Hokkaido Agricultural Mutual Relief Association, Hokkaido, Japan

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, and

A. Ogata

A. Ogata ³Veterinary Clinical Center, Hokkaido Agricultural Mutual Relief Association, Hokkaido, Japan

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Type:: Chapter

Pages:: 155–158

DOI:: https://doi.org/10.3920/9789086865505_019

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Staphylococcus aureus (SA) is the major pathogen causing chronic mastitis in cows. The roles of host immune system components in the defence of the mammary gland against infection are not well understood. Expression of adhesion molecules and lymphocyte markers and the functional responses of leucocytes from SA-infected cows were determined to elucidate the association between decreased host defence and SA infection. Eight Holstein lactating cows infected with SA were used for this study. Expression of adhesion molecules such as CD18, L-selectin and lymphocyte receptors, CD4,8 and surface IgG, on blood and milk leucocytes from cows with SA infection were determined by immunofluorescent analysis. Matrix metalloproteinases (MMP)2,9, lactoferrin (LF), acetyl glucosaminidase and somatic cells were measured to monitor the inflammatory response. LF and IgG concentrations in SA-infected quarter milk were 668.7ug/ml and 0.53mg/ml, respectively. LF, albumin and IgG and acid glycoproteins in mastitic milk were detected by immunoblotting. Activities for MMP-2,9 were clearly detected in SA-infected milk by zymography. L-selectin expression on neutrophils from SA-infected quarter milk showed a significant decrease compared to blood levels. Expression of CD18 on blood neutrophils in SA-infected cows was slightly increased compared to normal cows. Fc-receptor(FcR)-stimulated chemiluminescence (CL) of blood neutrophils was apparently decreased. SIg expression on blood lymphocytes from SA-infected cows appeared to be decreased compared with normal cows. Lymphocyte responses and FcR-stimulated neutrophil CL were found to be decreased in dairy cows with SA infection and may be at least in part associated with decreased host resistance capability in chronic SA infection.

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Mastitis in dairy production

Current knowledge and future solutions

E-Book ISBN:: 9789086865505

Publisher:: Wageningen Academic

Print Publication Date:: 24 Oct 2005

Subjects
- Life Sciences

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Changes in leucocyte surface receptor expression and functional responses in dairy cows with Staphylococcus aureus infection

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Mastitis in dairy production

Current knowledge and future solutions

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