Pathogenesis of chronic intramammary Escherichia coli infections

Belgin Dogan; Suzanne Klaessig; Kenneth Simpson; Steve Oliver; Raul Almeida; Ynte H. Schukken

Pathogenesis of chronic intramammary Escherichia coli infections

in Mastitis in dairy production

Autor:innen:

Belgin Dogan

Belgin Dogan ¹Quality Milk Production Services, Cornell University, Ithaca, USA

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Suzanne Klaessig

Suzanne Klaessig ¹Quality Milk Production Services, Cornell University, Ithaca, USA

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Kenneth Simpson

Kenneth Simpson ²Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, USA

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Steve Oliver

Steve Oliver ³Department of Animal Science and the Food Safety Center of Excellence, University of Tennessee, Knoxville, USA

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Raul Almeida

Raul Almeida ³Department of Animal Science and the Food Safety Center of Excellence, University of Tennessee, Knoxville, USA

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, und

Ynte H. Schukken

Ynte H. Schukken ¹Quality Milk Production Services, Cornell University, Ithaca, USA

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Art:: Kapitel

Seiten:: 129–136

DOI:: https://doi.org/10.3920/9789086865505_015

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Chronic intramammary Escherichia coli infections are an increasingly important problem in dairy herds. In this study we investigate the pathogenesis of chronic coliform mastitis. Three well-defined E. coli strains associated with chronic infections and three well-defined E. coli strains associated with acute infections were used as experimental pathogenic strains. Our results showed that the E. coli strains internalized by bovine mammary epithelial cells (MAC-T). This could provide E. coli with a survival advantage, allowing the microbes to better resist detection and clearance by both innate and adaptive immune defense mechanisms. By using gentamycin protection assay and immunofluorescence staining we showed that E. coli strains associated with chronic infections internalized mammary epithelial cells more efficiently than strains associated with transient infections. Antibacterial resistance was much higher among E. coli strains associated with acute infections. We demonstrated that internalization of mammary epithelial cells by E. coli was an active process involving host cell cytoskeletal rearrangement and intracellular signaling cascades. Two cytoskeletal disrupting agents, cytochalasin D and colchicine inhibited the internalization of E. coli into mammary epithelial cells, which indicates both actin filaments and microtubules play a role in the uptake process. Genistein and wortmannin both inhibit internalization. Polymerase chain reaction analysis showed the presence of type-1 pilus adhesin, FimH in strains used. Blocking FimH with mannose decreased bacterial adherence and internalization.

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Mastitis in dairy production

Current knowledge and future solutions

ISBN:: 9789086865505

Verleger:: Wageningen Academic

Print-Publikationsdatum:: 24 Oct 2005

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Pathogenesis of chronic intramammary Escherichia coli infections

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Mastitis in dairy production

Current knowledge and future solutions

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