Leukocyte dynamics and cytokine expression in response to mycobacterial water soluble compounds in bovine mastitis

Reena Mukherjee

Leukocyte dynamics and cytokine expression in response to mycobacterial water soluble compounds in bovine mastitis

In: Mastitis in dairy production

Author:

Reena Mukherjee

Reena Mukherjee Division of Medicine, Indian Veterinary Research Institute, Izatnagar, India

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Type:: Chapter

Pages:: 314–320

DOI:: https://doi.org/10.3920/9789086865505_045

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The epidemiological scenario of bovine mastitis in recent years is associated with impaired immune responses. The therapeutic and immunomodulatary potential of watersoluble fraction (WSF) of Mycobacterium phlei in subclinical mastitis (SCM) was studied. Forty-five cows screened for mastitis divided in three equal groups. Group I cows (15), negative for intramammary infection (IMI) served as healthy control, group II and III (30 cows) positive for IMI were injected with 100 μgm of WSF and 5 ml sterile phosphate buffer saline (PBS)via teat canal on 3 alternate days, respectively. Somatic cell count (SCC) and total bacterial count (TBC) was studied at 0,3,7,15 and 30 days post treatment (PT). Milk differential leukocyte count (DLC), phagocytic activity (PA) and phagocytic index (PI) of the milk polymorphonuclear cells and cytokine transcripts elaborated by peripheral blood lymphocytes culture (PBL) using reverse transcription polymerase chain reaction was observed at ‘0’ and 7 days PT. Treatment with WSF significantly (P<0.05) reduced the SCC and TBC on 15 and 30 days PT.Milk DLC revealed enhancement of lymphocyte %, similarly the PA and PI enhanced significantly on 7 days PT. PBL isolated from blood samples in group II showed mRNA expression of Interferon- γ (IFN-γ) and Interleukin-2 (IL-2). Reduction in SCC and TBC, enhancement of lymphocyte %, phagocytic activity and phagocytic index of milk leukocytes and expression of IFN-γ and IL-2 indicates the immunomodulatary potential of the adjuvant prepared from nonpathogenic strain of M.phlei. This is the first preliminary study on intramammary use of water soluble fraction in bovine mastitis, hence, can be used as adjunct or alternative to antibiotics in order to reduce the antibiotic residue in human food chain.

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Mastitis in dairy production

Current knowledge and future solutions

E-Book ISBN:: 9789086865505

Publisher:: Wageningen Academic

Print Publication Date:: 24 Oct 2005

Subjects
- Life Sciences

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Leukocyte dynamics and cytokine expression in response to mycobacterial water soluble compounds in bovine mastitis

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Mastitis in dairy production

Current knowledge and future solutions

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