Detection and diagnosis of bacterial wetwood in Tilia americana and Ulmus americana sapwood using a CP electronic-nose

A.D. Wilson

Detection and diagnosis of bacterial wetwood in Tilia americana and Ulmus americana sapwood using a CP electronic-nose

In: Industrial, medical and environmental applications of microorganisms

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A.D. Wilson

A.D. Wilson Forest Insect and Disease Research, Southern Hardwoods Laboratory, Center for Bottomland Hardwoods Research, Southern Research Station, USDA Forest Service, P.O. Box 227, Stoneville, MS 38776, USA; dwilson02@fs.fed.us

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Type:: Chapter

Pages:: 209–214

DOI:: https://doi.org/10.3920/9789086867950_036

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Electronic-nose (e-nose) methods for detecting bacterial wetwood were developed and tested using a conducting polymer (CP)-type electronic nose, the Aromascan A32S, to diagnose the disease in two hardwood species (Tilia americana and Ulmus americana) based on headspace volatile metabolites in sapwood. An aroma library was developed using diagnostic aroma signature patterns (profile databases) derived from e-nose analysis of known healthy and wetwood-infected sapwood cores of each tree species. The library was used to screen sapwood cores for the presence of wetwood in unknown samples. The A32S e-nose effectively distinguished between differences in headspace volatiles from tree cores of different sample types, correctly identifying them at frequencies ranging from 88.1-100%. The distribution of aroma class components, based on artificial neural net training and principal component analysis (PCA), indicated the relatedness and differences in headspace volatiles of aroma classes represented by each sample type. Significant differences were found between the aroma profiles of healthy vs. wetwoodinfected sapwood of basswood and American elm, and even greater differences between the headspace wood volatiles released from the two wood types.

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Industrial, medical and environmental applications of microorganisms

Current status and trends

E-Book ISBN:: 9789086867950

Publisher:: Wageningen Academic

Print Publication Date:: 01 Nov 2014

Subjects
- Life Sciences

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Detection and diagnosis of bacterial wetwood in Tilia americana and Ulmus americana sapwood using a CP electronic-nose

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Industrial, medical and environmental applications of microorganisms

Current status and trends

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