Survival and persistence of Bacteroides species as faecal indicators and the recovery of 16S rRNA markers under controlled conditions

K.R. Hussein; P.L. Waines; G. Glegg; G. Bradley

Survival and persistence of Bacteroides species as faecal indicators and the recovery of 16S rRNA markers under controlled conditions

In: Industrial, medical and environmental applications of microorganisms

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K.R. Hussein

K.R. Hussein ¹School of Biological Sciences, Plymouth University, Devon PL4 8AA, UK; khwam.hussein@plymouth.ac.uk

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P.L. Waines

P.L. Waines ¹School of Biological Sciences, Plymouth University, Devon PL4 8AA, UK; khwam.hussein@plymouth.ac.uk

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G. Glegg

G. Glegg ²School of Marine Sciences and Engineering, Plymouth University, Devon PL4 8AA, UK

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G. Bradley

G. Bradley ¹School of Biological Sciences, Plymouth University, Devon PL4 8AA, UK; khwam.hussein@plymouth.ac.uk

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Type:: Chapter

Pages:: 191–196

DOI:: https://doi.org/10.3920/9789086867950_033

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The persistence of faecal Bacteroides species after release into differing environmental waters and sediments is not well understood. This 18 day study aimed to determine the survival (using culturebased method) and PCR-based marker recovery of human- and dog-sourced Bacteroides from river and seawater laboratory microcosms containing muddy sediment. The survival of Bacteroides in the water declined very sharply from 1.2×104 cfu/100 ml to near zero between day 6 and day 14; likewise for those in the sediment a rapid decline was observed from 8.0×103 cfu/gram to near zero between day 8 and day 16. Generic, human and dog Bacteroides PCR primers were used to evaluate the persistence of faecal Bacteroides markers in these controlled conditions and the rates of decline were similar to culture-based recovery. The similarity in persistence of these Bacteroides 16S rRNA genetic markers in environmental water and sediment suggests that viable but non-culturable (VBNC) Bacteroides do not persist in the natural environment for long and therefore that 16S rRNA markers can thus be of value as faecal indicators of bathing water pollution and in source tracking.

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Industrial, medical and environmental applications of microorganisms

Current status and trends

E-Book ISBN:: 9789086867950

Publisher:: Wageningen Academic

Print Publication Date:: 01 Nov 2014

Subjects
- Life Sciences

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Survival and persistence of Bacteroides species as faecal indicators and the recovery of 16S rRNA markers under controlled conditions

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Industrial, medical and environmental applications of microorganisms

Current status and trends

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