Easy and rapid detection and identification of yeasts in winemaking samples by flow cytometry and/or FISH

O. Lucio; S. Ferrer; I. Pardo

Easy and rapid detection and identification of yeasts in winemaking samples by flow cytometry and/or FISH

于Industrial, medical and environmental applications of microorganisms

著者：

O. Lucio

O. Lucio ENOLAB, Dpto. De Microbiologia y Ecologia, Facultad de Biologia, Universidad de Valencia, ERI-ISIC BIOTECMED/IviSoCa, Campus de Burjassot, 46100 Valencia, Spain; olga.lucio@uv.es

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S. Ferrer

S. Ferrer ENOLAB, Dpto. De Microbiologia y Ecologia, Facultad de Biologia, Universidad de Valencia, ERI-ISIC BIOTECMED/IviSoCa, Campus de Burjassot, 46100 Valencia, Spain; olga.lucio@uv.es

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, and

I. Pardo

I. Pardo ENOLAB, Dpto. De Microbiologia y Ecologia, Facultad de Biologia, Universidad de Valencia, ERI-ISIC BIOTECMED/IviSoCa, Campus de Burjassot, 46100 Valencia, Spain; olga.lucio@uv.es

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类型:: 章节

Page Range:: 277–282

DOI:: https://doi.org/10.3920/9789086867950_048

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Flow cytometry (FC) can be considered a useful method for microbiological quality control in wineries, and for the investigation of the growth dynamics of microorganisms in wine. In this work, we have adapted previous fluorescence in situ hybridization (FISH) protocols for yeasts to FC, optimizing at the same time a liquid hybridization protocol. For this, we used specific fluorescein-labelled oligonucleotide probes targeted to the D1/D2 region of the 26S rRNA of different yeast species known to occur frequently in wine, both spoilage and beneficial. To improve the hybridization protocol, we tested different parameters such as yeast growth, fixation and permeabilization. One important advantage is that the hybridized yeasts in liquid can be now examined under a microscope (FISH) and/or by FC. Hybridization with fluorescent probes is extremely specific, which facilitates the identification and counting of the different species of yeasts in the same wine with a single analysis.

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Industrial, medical and environmental applications of microorganisms

Current status and trends

ISBN:: 9789086867950

出版社:: Wageningen Academic

印刷出版日期:: 01 Nov 2014

Subjects
- Life Sciences

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Easy and rapid detection and identification of yeasts in winemaking samples by flow cytometry and/or FISH

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Industrial, medical and environmental applications of microorganisms

Current status and trends

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