Simultaneous determination of aflatoxin, ochratoxin A and zearalenone by a new immunoaffinity column

N. Zabe; E. Wadleigh; B.A. Cohen

Simultaneous determination of aflatoxin, ochratoxin A and zearalenone by a new immunoaffinity column

In: Mycotoxins and phycotoxins

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N. Zabe

N. Zabe VICAM, 313 Pleasant St., Watertown, MA 02472, USA; nancyz@vicam.com

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E. Wadleigh

E. Wadleigh VICAM, 313 Pleasant St., Watertown, MA 02472, USA; nancyz@vicam.com

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, and

B.A. Cohen

B.A. Cohen VICAM, 313 Pleasant St., Watertown, MA 02472, USA; nancyz@vicam.com

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Type:: Chapter

Pages:: 73–81

DOI:: https://doi.org/10.3920/9789086865857_007

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Existing clean up methods for HPLC determination of mycotoxins require separate analysis for aflatoxin, ochratoxin A, and zearalenone. Described is a method for simultaneous determination of aflatoxin, ochratoxin A, and zearalenone using one sample clean up and one HPLC run. A sample is extracted in aqueous methanol. The extract is diluted with buffer and passed over an immunoaffinity column containing immobilised antibodies to aflatoxin, ochratoxin A, and zearalenone (AOZ HPLC™ column). The column is then washed and eluted. Using gradient flow and wavelength switching for detection, aflatoxins, ochratoxin A and zearalenone were measured in a single HPLC run of less than 30 minutes. Recoveries for all toxins were greater than 80% from samples of spiked maize, sorghum and wheat over the range of 0-100μg/kg total aflatoxin, 0-1,000 μg/kg zearalenone and 0-100 μg/kg ochratoxin A. Analysis using multiple mycotoxin immunoaffinity columns can save time and money in performing the mycotoxin analysis required by many countries worldwide.

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Mycotoxins and phycotoxins

Advances in determination, toxicology and exposure management

E-Book ISBN:: 9789086865857

Publisher:: Wageningen Academic

Print Publication Date:: 30 Oct 2006

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Simultaneous determination of aflatoxin, ochratoxin A and zearalenone by a new immunoaffinity column

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Mycotoxins and phycotoxins

Advances in determination, toxicology and exposure management

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