Analytical characterisation of degradation products of fumonisin B1 by LC-MS/MS

E. Vekiru; M. Täubel; G. Schatzmayr; R. Schuhmacher; E.M. Binder; R. Krska

Analytical characterisation of degradation products of fumonisin B₁ by LC-MS/MS

In: Mycotoxins and phycotoxins

Authors:

E. Vekiru

E. Vekiru ¹Center for Analytical Chemistry and Christian Doppler Laboratory for Mycotoxin Research, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences Vienna, Konrad Lorenz Strasse. 20, A-3430 Tulln, Austria; rudolf.krska@boku.ac.at

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M. Täubel

M. Täubel ²Biomin GmbH, Industrie Strasse 21, A-3130 Herzogenburg, Austria

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G. Schatzmayr

G. Schatzmayr ²Biomin GmbH, Industrie Strasse 21, A-3130 Herzogenburg, Austria

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R. Schuhmacher

R. Schuhmacher ¹Center for Analytical Chemistry and Christian Doppler Laboratory for Mycotoxin Research, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences Vienna, Konrad Lorenz Strasse. 20, A-3430 Tulln, Austria; rudolf.krska@boku.ac.at

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E.M. Binder

E.M. Binder ²Biomin GmbH, Industrie Strasse 21, A-3130 Herzogenburg, Austria

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, and

R. Krska

R. Krska ¹Center for Analytical Chemistry and Christian Doppler Laboratory for Mycotoxin Research, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences Vienna, Konrad Lorenz Strasse. 20, A-3430 Tulln, Austria; rudolf.krska@boku.ac.at

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Type:: Chapter

Pages:: 107–116

DOI:: https://doi.org/10.3920/9789086865857_011

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Fumonisin B₁ (FB₁), first reported in 1988 in South Africa, is the predominant fumonisin among a group of structurally related fumonisins. It occurs in mainly maize and maize-based products. FB₁ has been shown to produce a wide range of pathological effects in animals, including leukoencephalomalacia in horses and pulmonary oedema in swine. It also disrupts sphingolipid biosynthetic pathways in animals and plant cells. It is hence understandable that there is considerable interest in the detoxification of FB₁. Decontamination using selected micro-organisms with the ability to degrade or biotransform mycotoxins has many advantages. Blackwell _{et al.} (1999) showed that the fungus _{Exophiala spinifera} produces enzymes that metabolise fumonisins. This fumonisin-metabolism consists of de-esterification of fumonisins tricarballylic acid esters followed by oxidative deamination of the resulting amine alcohol backbone resulting in a 2-oxo-12,16-dimethyl-3,5,10,14,15-icosanepentol (2-OP1) that undergoes internal cyclisation to form a hemiketal. Since hydrolysed fumonisin B₁ (HFB₁) can easily be adsorbed and is as toxic as or even more toxic than FB₁, a study on other micro-organisms with the capability to detoxify FB₁ and HFB₁ was conducted. As a first step, reported here, samples were analysed after degradation with E. spinifera. Using LC-MS/MS for characterisation of the samples, we could reconstruct the transformation of FB₁ over partially hydrolysed FB₁ to completely hydrolysed FB₁, which was then further metabolised. This metabolism led to a degradation product HFB₁, which showed a mass spectrum with similar molecular ion signal and fragmentation pattern like 2-OP1. To characterise the product we performed full scan and also product ion scan experiments in both, positive and negative ionisation mode. Detoxification of the degraded samples was also demonstrated in a bioassay.

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Mycotoxins and phycotoxins

Advances in determination, toxicology and exposure management

E-Book ISBN:: 9789086865857

Publisher:: Wageningen Academic

Print Publication Date:: 30 Oct 2006

Subjects
- Life Sciences

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Analytical characterisation of degradation products of fumonisin B1 by LC-MS/MS

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Mycotoxins and phycotoxins

Advances in determination, toxicology and exposure management

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Analytical characterisation of degradation products of fumonisin B₁ by LC-MS/MS