Determination of masked mycotoxins using HPLCâtandem mass spectrometry

F. Berthiller; R. Schuhmacher; B. Poppenberger; D. Lucyshyn; M. Lemmens; G. Adam; R. Krska

Determination of masked mycotoxins using HPLCâtandem mass spectrometry

In: Mycotoxins and phycotoxins

Authors:

F. Berthiller

F. Berthiller ¹Christian Doppler Laboratory for Mycotoxin Research, Center for Analytical Chemistry, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences, Vienna, Konrad Lorenz Str. 20, A-3430 Tulln, Austria; rudolf.krska@boku.ac.at

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R. Schuhmacher

R. Schuhmacher ¹Christian Doppler Laboratory for Mycotoxin Research, Center for Analytical Chemistry, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences, Vienna, Konrad Lorenz Str. 20, A-3430 Tulln, Austria; rudolf.krska@boku.ac.at

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B. Poppenberger

B. Poppenberger ²Institute of Applied Genetics and Cell Biology, Department of Applied Plant Sciences and Plant Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Muthgasse 18, A-1190 Vienna, Austria

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D. Lucyshyn

D. Lucyshyn ²Institute of Applied Genetics and Cell Biology, Department of Applied Plant Sciences and Plant Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Muthgasse 18, A-1190 Vienna, Austria

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M. Lemmens

M. Lemmens ³Plant Production Biotechnology, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences, Vienna, Konrad Lorenz Str. 20, A-3430 Tulln, Austria

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G. Adam

G. Adam ²Institute of Applied Genetics and Cell Biology, Department of Applied Plant Sciences and Plant Biotechnology, University of Natural Resources and Applied Life Sciences, Vienna, Muthgasse 18, A-1190 Vienna, Austria

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R. Krska

R. Krska ¹Christian Doppler Laboratory for Mycotoxin Research, Center for Analytical Chemistry, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences, Vienna, Konrad Lorenz Str. 20, A-3430 Tulln, Austria; rudolf.krska@boku.ac.at

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Type:: Chapter

Pages:: 125â132

DOI:: https://doi.org/10.3920/9789086865857_013

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The topic of conjugated mycotoxins first caught the attention of investigators in the mid eighties of the last century because in some cases of mycotoxicoses, clinical observations in animals did not correlate with the low mycotoxin content determined in the corresponding feed. High performance liquid chromatography combined with tandem mass spectrometry offers a powerful tool for identification and characterization of polar non-volatile mycotoxin conjugates. Derivatives can be identified due to their molecular mass and their fragmentation behaviour. Moreover, known conjugates can be quantified and plant extracts can be screened for unknown mycotoxin conjugates with different MS/MS techniques. We chemically synthesized deoxynivalenol-3-Î²-D-glucopyranoside, deoxynivalenol-15-Î²-D-glucopyranoside and zearalenone-4-Î²-D-glucopyranoside. The two deoxynivalenol-glucosides showed different fragmentation behaviours on a hybrid quadrupole â linear ion trap tandem mass spectrometer and could therefore be distinguished. It was possible to confirm the identity of deoxynivalenol-3-glucoside in deoxynivalenol treated yeast, which expressed the Arabidopsis thaliana glucosyltransferase DOGT1. Furthermore, deoxynivalenol-3-glucoside was detected in deoxynivalenol treated Arabidopsis and wheat. We also confirmed the formation of ZON-4-glucoside, a substance that was already known to occur in contaminated wheat. Finally, we isolated and characterized zearalenone-4-sulfate from Fusarium inoculated rice.

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Mycotoxins and phycotoxins

Advances in determination, toxicology and exposure management

E-Book ISBN:: 9789086865857

Publisher:: Wageningen Academic

Print Publication Date:: 30 Oct 2006

Subjects
- Life Sciences

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Determination of masked mycotoxins using HPLCâtandem mass spectrometry

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Mycotoxins and phycotoxins

Advances in determination, toxicology and exposure management

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Determination of masked mycotoxins using HPLCâtandem mass spectrometry