Optimization of Neisseria lactamicaâs outer membrane vesicles production in batch cultivation process

M.W. Garcia1; B.I. GonÃ§alves; G.F.C.L. Salustiano; P.L. Ho; R.P.F. Schenkman

Optimization of Neisseria lactamicaâs outer membrane vesicles production in batch cultivation process

äºIndustrial, medical and environmental applications of microorganisms

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M.W. Garcia1

M.W. Garcia1 ¹Centro de Biotecnologia, Instituto Butantan, IBu, Av. Vital Brazil, 1500, 05503-900 SÃ£o Paulo, SP, Brazil; mariana_watanabe@usp.br
²Universidade de SÃ£o Paulo, USP, Av. Prof. Lineu Prestes, 2415, 05508-000 SÃ£o Paulo, SP, Brazil

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B.I. GonÃ§alves

B.I. GonÃ§alves ¹Centro de Biotecnologia, Instituto Butantan, IBu, Av. Vital Brazil, 1500, 05503-900 SÃ£o Paulo, SP, Brazil; mariana_watanabe@usp.br

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G.F.C.L. Salustiano

G.F.C.L. Salustiano ¹Centro de Biotecnologia, Instituto Butantan, IBu, Av. Vital Brazil, 1500, 05503-900 SÃ£o Paulo, SP, Brazil; mariana_watanabe@usp.br
²Universidade de SÃ£o Paulo, USP, Av. Prof. Lineu Prestes, 2415, 05508-000 SÃ£o Paulo, SP, Brazil

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P.L. Ho

P.L. Ho ¹Centro de Biotecnologia, Instituto Butantan, IBu, Av. Vital Brazil, 1500, 05503-900 SÃ£o Paulo, SP, Brazil; mariana_watanabe@usp.br

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R.P.F. Schenkman

R.P.F. Schenkman ¹Centro de Biotecnologia, Instituto Butantan, IBu, Av. Vital Brazil, 1500, 05503-900 SÃ£o Paulo, SP, Brazil; mariana_watanabe@usp.br

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Page Range:: 664â669

DOI:: https://doi.org/10.3920/9789086867950_119

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Outer membrane vesicles (OMV) are released by evaginations of the outer membrane of Gram negative bacteria and have gained attention with respect to their biological functions, role in pathogenesis and use in new vaccines strategies and combinations. OMV from Neisseria lactamica induce antibodies that crossreact with Neisseria meningitidis and can have a potential mucosal adjuvant property. The objective of this work was to optimize OMV production from N. lactamica through the modification and improvement of its original culture medium, meningococcus defined medium (MCDA). Experiments were carried out in batch processes which had their concentration of lactate, amino acids and yeast extract (YE) modified according to each essay. Results indicated that the use of threefold of MCDAâs concentration of lactate and double of its concentration of amino acids plus 2 g/l of YE seems to be the most favourable condition in both terms of cost and productivity of OMV. This proportion between carbon and nitrogen sources appears to be the most adequate for N. lactamicaâs metabolism.

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Industrial, medical and environmental applications of microorganisms

Current status and trends

ISBN:: 9789086867950

åºçç¤¾:: Wageningen Academic

å°å·åºçæ¥æ:: 01 Nov 2014

Subjects
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Optimization of Neisseria lactamicaâs outer membrane vesicles production in batch cultivation process

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