Inactivation and identification of Bacillus anthracis spores

M. Gil García; I. Peraile Muñoz; M.V. Jiménez Pérez; J.C. Cabria Ramos; P. Lorenzo Lozano

Inactivation and identification of Bacillus anthracis spores

In: Industrial, medical and environmental applications of microorganisms

Authors:

M. Gil García

M. Gil García ¹Biological Defence Unit, NBC and Materials Area, Technological Institute of La Marañosa, Ministry of Defence, San Martín de la Vega, km 10.5, 28330, Madrid, Spain; plorloz@oc.mde.es

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I. Peraile Muñoz

I. Peraile Muñoz ²Systems Engineering for the Defence of Spain (ISDEFE), Calle de Beatriz de Bobadilla, 3, 28040 Madrid, Spain

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M.V. Jiménez Pérez

M.V. Jiménez Pérez ¹Biological Defence Unit, NBC and Materials Area, Technological Institute of La Marañosa, Ministry of Defence, San Martín de la Vega, km 10.5, 28330, Madrid, Spain; plorloz@oc.mde.es

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J.C. Cabria Ramos

J.C. Cabria Ramos ¹Biological Defence Unit, NBC and Materials Area, Technological Institute of La Marañosa, Ministry of Defence, San Martín de la Vega, km 10.5, 28330, Madrid, Spain; plorloz@oc.mde.es

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P. Lorenzo Lozano

P. Lorenzo Lozano ¹Biological Defence Unit, NBC and Materials Area, Technological Institute of La Marañosa, Ministry of Defence, San Martín de la Vega, km 10.5, 28330, Madrid, Spain; plorloz@oc.mde.es

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Type:: Chapter

Pages:: 659–663

DOI:: https://doi.org/10.3920/9789086867950_118

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Bacillus anthracis endospores must be handled at a biosafety level-3 (BSL-3) containment facility, therefore an appropriate inactivation procedure is essential in order to both detect them in a reliable and rapid way and to avoid human infections from antrax-contamined samples if a biosafety level-3 laboratory is not available. Unfortunately, dormant spores exhibit incredible hardiness against germicidal agents. The most effective methods for inactivating spores are usually the chemical treatment, heat inactivation (boiling, moist heat, dry heat) and irradiation (microwave, UV, gamma, electron beam). The inactivation method used must alter the cell structure as little as possible in order to ensure a correct diagnosis by different techniques. Several kinds of physical and chemical inactivation methods were assessed in order to define the conditions that ensure inactivation of endospores of B. anthracis 34F2 Sterne and permit, at the same time, its identification by PCR and immunoassays techniques.

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Industrial, medical and environmental applications of microorganisms

Current status and trends

E-Book ISBN:: 9789086867950

Publisher:: Wageningen Academic

Print Publication Date:: 01 Nov 2014

Subjects
- Life Sciences

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Inactivation and identification of Bacillus anthracis spores

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Industrial, medical and environmental applications of microorganisms

Current status and trends

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