Poly-Î²-hydroxybutyrate accumulation in Bradyrhizobium japonicum depends on proteins referred to as phasins

K. Yoshida; A. Motokubota; K. Tanaka; S. Takenaka

Poly-Î²-hydroxybutyrate accumulation in Bradyrhizobium japonicum depends on proteins referred to as phasins

In: Industrial, medical and environmental applications of microorganisms

Authors:

K. Yoshida

K. Yoshida ¹Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada, Kobe 657 8501, Japan; kenyoshi@kobe-u.ac.jp
²Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe 657 8501, Japan

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A. Motokubota

A. Motokubota ¹Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada, Kobe 657 8501, Japan; kenyoshi@kobe-u.ac.jp

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K. Tanaka

K. Tanaka ²Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe 657 8501, Japan

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S. Takenaka

S. Takenaka ¹Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai, Nada, Kobe 657 8501, Japan; kenyoshi@kobe-u.ac.jp
²Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, Nada, Kobe 657 8501, Japan

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Type:: Chapter

Pages:: 470â475

DOI:: https://doi.org/10.3920/9789086867950_083

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Bradyrhizobium japonicum USDA110, a soybean symbiont, is capable of accumulating a large amount of poly-Î²-hydroxybutyrate (PHB) as an intracellular carbon storage polymer during free-living growth. Within the genome of USDA110, there are a number of genes annotated as paralogs of proteins involved in PHB metabolism, including its biosynthesis, degradation, and stabilization of its granules. They include two phbA paralogs, two phbB paralogs, five phbC paralogs, two phaZ paralogs, at least four phaP phasin paralogs, and one phaR encoding a putative transcriptional repressor to control phaP expression. Quantitative reverse-transcriptase PCR analyses revealed that PHB accumulation was related neither to redundancy nor expression levels of the phbA, phbB, phbC, and phaZ paralogs for PHB-synthesis and degradation. On the other hand, at least three of the phaP paralogs, involved in the growth and stabilization of PHB granules, were induced under PHB accumulating conditions. Moreover, the most prominently induced phasin exhibited the highest affinity to PHB in vitro; it was able to displace PhaR previously bound to PHB. These results suggest that PHB accumulation in free-living B. japonicum USDA110 may not be achieved by controlling production and degradation of PHB. In contrast, it is achieved by stabilizing granules autonomously produced in an environment of excess carbon sources together with restricted nitrogen sources.

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Industrial, medical and environmental applications of microorganisms

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E-Book ISBN:: 9789086867950

Publisher:: Wageningen Academic

Print Publication Date:: 01 Nov 2014

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- Life Sciences

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Poly-Î²-hydroxybutyrate accumulation in Bradyrhizobium japonicum depends on proteins referred to as phasins

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Industrial, medical and environmental applications of microorganisms

Current status and trends

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