Economic liquid growth medium development for high-rate production of cellular biomass and lactic acid of Lactococcus lactis

M.-P. Zacharof; R.W. Lovitt

Economic liquid growth medium development for high-rate production of cellular biomass and lactic acid of Lactococcus lactis

In: Industrial, medical and environmental applications of microorganisms

Authors:

M.-P. Zacharof

M.-P. Zacharof ¹Centre for Complex Fluid Processing (CCFP), College of Engineering, Swansea University, Talbot building, Swansea, SA2 8PP, United Kingdom; myrtozacharof1981@yahoo.com
²Multidisciplinary Nanotechnology Center (MNC), College of Engineering, Swansea University, Swansea, Talbot building SA2 8PP, United Kingdom
³Centre for Water Advanced Technologies and Environmental Research (CWATER), College of Engineering, Talbot building, Swansea University, Swansea, SA2 8PP, United Kingdom

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R.W. Lovitt

R.W. Lovitt ¹Centre for Complex Fluid Processing (CCFP), College of Engineering, Swansea University, Talbot building, Swansea, SA2 8PP, United Kingdom; myrtozacharof1981@yahoo.com
²Multidisciplinary Nanotechnology Center (MNC), College of Engineering, Swansea University, Swansea, Talbot building SA2 8PP, United Kingdom
³Centre for Water Advanced Technologies and Environmental Research (CWATER), College of Engineering, Talbot building, Swansea University, Swansea, SA2 8PP, United Kingdom

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Type:: Chapter

Pages:: 419–424

DOI:: https://doi.org/10.3920/9789086867950_074

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Lactococcus lactis importance in the alimentary and pharmaceutical industry as a lactic acid and nisin production strain has been well established. However for its growth, nutrient media such as M17 and Man de Rogosa Sharp (MRS) are used, which are not suitable for use in large quantities due to the high preparation cost and content of nitrogen sources of beef extract and peptone from poultry that are not environmentally friendly and have possible health risks. For its intensive propagation and high rate lactic acid production an economic liquid growth medium was formulated, using L. lactis NCIMB 700185. To test medium efficiency, the coccus was grown in a 2 L CSTR with continuous temperature and pH control. The developed medium supported evenly cellular growth offering elevated maximum yields incorporating only the chemicals compounds needed, resulting in an improvement of the growth rate of the coccus of 70.6%, when compared to the same strain on MRS, the lactic acid concentration produced was 30.28 g/l.

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Industrial, medical and environmental applications of microorganisms

Current status and trends

E-Book ISBN:: 9789086867950

Publisher:: Wageningen Academic

Print Publication Date:: 01 Nov 2014

Subjects
- Life Sciences

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Economic liquid growth medium development for high-rate production of cellular biomass and lactic acid of Lactococcus lactis

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Industrial, medical and environmental applications of microorganisms

Current status and trends

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