Viability of Lactobacillus acidophilus and Bifidobacter breve encapsulated into polysaccharide particles

P. Matoušková; J. Hurtová; A. Lichnová; P. Benešová; S. Obruča; I. Márová

Viability of Lactobacillus acidophilus and Bifidobacter breve encapsulated into polysaccharide particles

In: Industrial, medical and environmental applications of microorganisms

Authors:

P. Matoušková

P. Matoušková ¹Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic; xcmatouskovap@fch.vutbr.cz
²Department of Food Chemistry and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic

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J. Hurtová

J. Hurtová ¹Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic; xcmatouskovap@fch.vutbr.cz
²Department of Food Chemistry and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic

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A. Lichnová

A. Lichnová ¹Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic; xcmatouskovap@fch.vutbr.cz
²Department of Food Chemistry and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic

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P. Benešová

P. Benešová ¹Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic; xcmatouskovap@fch.vutbr.cz
²Department of Food Chemistry and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic

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S. Obruča

S. Obruča ¹Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic; xcmatouskovap@fch.vutbr.cz

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, and

I. Márová

I. Márová ¹Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic; xcmatouskovap@fch.vutbr.cz
²Department of Food Chemistry and Biotechnology, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 612 00 Brno, Czech Republic

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Type:: Chapter

Pages:: 391–396

DOI:: https://doi.org/10.3920/9789086867950_069

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Probiotics can provide beneficial effects on the human body by keeping the healthy gut microflora, stimulating the immune system, synthesizing vitamins and antimicrobial agents and improving the absorption of calcium. Probiotics are microorganisms that are very sensitive to adverse environments. Thus, new methods to preserve the viability of probiotics are developed. Microencapsulation is technology which has been used to protection of these bacterial cells. In this work possibilities of encapsulation of probiotic bacteria were tested. Cells were packaged into polysaccharide particles. The size and shape of the beads was determined using light microscopy. Probiotic strains Bifidobacterium breve and Lactobacillus acidophilus were used for encapsulation. Materials tested for particles preparation were alginate, chitosan, starch, pullulan and mixtures of some polysaccharides. Particles stability was tested in different conditions: in water, in model foods and artificial body fluids. Polysaccharide particles showed a very good stability in body fluids as well as in model foods. Viability of microorganisms was determined using flow cytometry and fluorescence microscopy. Finally, viability of encapsulated cells during lyophilization and storage was tested. Encapsulation of probiotic bacteria could preserve their viability and long-term survival until the product expiration date and their survival in gastro-intestinal tract.

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Industrial, medical and environmental applications of microorganisms

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E-Book ISBN:: 9789086867950

Publisher:: Wageningen Academic

Print Publication Date:: 01 Nov 2014

Subjects
- Life Sciences

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Viability of Lactobacillus acidophilus and Bifidobacter breve encapsulated into polysaccharide particles

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Industrial, medical and environmental applications of microorganisms

Current status and trends

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