Cross-reactivity of six zearalenone antibodies in a hand-held fluorescence polarisation immunoassay

C.M. Maragos; E.-K. Kim

Cross-reactivity of six zearalenone antibodies in a hand-held fluorescence polarisation immunoassay

In: Mycotoxins and phycotoxins

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C.M. Maragos

C.M. Maragos Mycotoxin Research Unit, National Center for Agricultural Utilization Research, USDA/ARS, 1815 N. University St., Peoria, IL 61604, USA; maragocm@ncaur.usda.gov

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E.-K. Kim

E.-K. Kim Mycotoxin Research Unit, National Center for Agricultural Utilization Research, USDA/ARS, 1815 N. University St., Peoria, IL 61604, USA; maragocm@ncaur.usda.gov

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Type:: Chapter

Pages:: 91–100

DOI:: https://doi.org/10.3920/9789086865857_009

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Zearalenone is an estrogenic mycotoxin produced by several fungi, in particular Fusarium graminearum, and is commonly found in grain throughout the world. The native fluorescence of zearalenone (ZEN) has been used to advantage for detection of this toxin by instrumental methods such as HPLC. Antibody-based screening assays such enzyme-linked immunosorbent assays (ELISAs) have existed for a number of years for determination of ZEN and related metabolites. While convenient, the ELISAs for small molecules such as ZEN require a washing step to separate the bound and unbound enzyme label before detection. Fluorescence polarisation (FP) immunoassays do not require a separation step and therefore, with fewer manipulations, have the potential to be more rapid. Previously we have reported a FP immunoassay for detection of ZEN in maize using a commercially available antibody. In the present report we describe the cross-reactivity and kinetics of interaction of one commercially available and 5 newly developed monoclonal antibodies for the mycotoxins ZEN, zearalanone, α-zearalenol, β-zearalenol, α-zearalanol, and β-zearalanol using a miniaturised version of the previous FP device. Using the cross-reactivity and kinetic data, particular antibodies with the desired analytical characteristics can be selected for further application.

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Mycotoxins and phycotoxins

Advances in determination, toxicology and exposure management

E-Book ISBN:: 9789086865857

Publisher:: Wageningen Academic

Print Publication Date:: 30 Oct 2006

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- Life Sciences

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Cross-reactivity of six zearalenone antibodies in a hand-held fluorescence polarisation immunoassay

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Mycotoxins and phycotoxins

Advances in determination, toxicology and exposure management

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