Evaluation of the immune response to vaccination against C. Pseudotubercolosis in an alpaca herd in Italy: Preliminary results

D. Beghelli; G.L. D’ Alterio; G. Severi; L. Moscati; G. Pezzotti; A. Foglini; L. Battistacci; M. Cagiola; C. Ayala Vargas

Evaluation of the immune response to vaccination against C. Pseudotubercolosis in an alpaca herd in Italy: Preliminary results

In: South American camelids research

Authors:

D. Beghelli

D. Beghelli ¹Facoltà di Medicina Veterinaria, Via Circonvallazione 93/95 62024 Matelica (MC), Italy; daniela.beghelli@unicam.it.

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G.L. D’ Alterio

G.L. D’ Alterio ²Libero Professionista, Studio Veterinario associato “Vannucci, Casarosa & D’ Alterio”, Manciano, Grosseto, Italy.

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G. Severi

G. Severi ³Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche, Via Salvemini 1, 06129 Perugia, Italy.

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L. Moscati

L. Moscati ³Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche, Via Salvemini 1, 06129 Perugia, Italy.

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G. Pezzotti

G. Pezzotti ³Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche, Via Salvemini 1, 06129 Perugia, Italy.

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A. Foglini

A. Foglini ³Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche, Via Salvemini 1, 06129 Perugia, Italy.

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L. Battistacci

L. Battistacci ³Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche, Via Salvemini 1, 06129 Perugia, Italy.

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M. Cagiola

M. Cagiola ³Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche, Via Salvemini 1, 06129 Perugia, Italy.

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, and

C. Ayala Vargas

C. Ayala Vargas ⁴Università Publica de El Alto, La Paz, Bolivia.

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Type:: Chapter

Pages:: 133–139

DOI:: https://doi.org/10.3920/978-90-8686-576-5_015

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The applicability of some assays to evaluate the effectiveness of the immune function, as previously used in other animal species (pigs, cows and sheep), was tested in an alpaca (Lama pacos) herd affected by Caseous Lymphadenitis (Corynebacterium pseudotubercolosis) and subject to a vaccination programme against the causative agent. The vaccination programme was initiated following failure of controlling the outbreak by isolation and treatment of the initially affected animals. An homotypic, autogenous inactivated and adjuvated (aluminium hydroxide) vaccine, administered subcutaneously at day 0 (A) and after 21 days (B) was used. Blood samples were collected by venipuncture of the jugular vein on days 0, 21 and 42 (C). Samples in EDTA, collected on day A and C, were analysed for standard haematological parameters. Serum obtained from whole blood with no anticoagulant on days A, B and C was tested for: Lysozyme serum titration (expressed in μg/m), serum Bactericidal assay (expressed in %), semiquantitative complement titration (expressed in CH50) and immunoglobuline (by electrophoresis) and IgGs concentrations (by Radial Immunodiffusion tests).

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South American camelids research

Volume 1

E-Book ISBN:: 9789086865765

Publisher:: Wageningen Academic

Print Publication Date:: 01 Nov 2006

Subjects
- Life Sciences
  - General
  - Animal & Veterinary

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Evaluation of the immune response to vaccination against C. Pseudotubercolosis in an alpaca herd in Italy: Preliminary results

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South American camelids research

Volume 1

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