Detection of mastitis pathogens by analysis of volatile metabolites

K.A. Hettinga; H.J.F. van Valenberg; T.J.G.M. Lam; A.C.M. van Hooijdonk

Detection of mastitis pathogens by analysis of volatile metabolites

于Mastitis control

著者：

K.A. Hettinga

K.A. Hettinga ¹Dairy Science and Technology group, Wageningen University and Research Centre, P.O. Box 8129, 6700 EV Wageningen, the Netherlands

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H.J.F. van Valenberg

H.J.F. van Valenberg ¹Dairy Science and Technology group, Wageningen University and Research Centre, P.O. Box 8129, 6700 EV Wageningen, the Netherlands

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T.J.G.M. Lam

T.J.G.M. Lam ²2GD Animal Health Service Ltd, P.O. Box 9, 7420 AA Deventer, the Netherlands Corresponding author: kasper.hettinga@wur.nl

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, and

A.C.M. van Hooijdonk

A.C.M. van Hooijdonk ¹Dairy Science and Technology group, Wageningen University and Research Centre, P.O. Box 8129, 6700 EV Wageningen, the Netherlands

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类型:: 章节

Page Range:: 199–203

DOI:: https://doi.org/10.3920/9789086866496_040

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The possibility to detect mastitis pathogens based on their volatile metabolites was studied. The 5 most important mastitis pathogens in the Netherlands (Staphylococcus aureus, coagulasenegative staphylococci, Streptococcus uberis, Streptococcus dysgalactiae, and Escherichia coli) were used in this study. Using a neural network, mastitis causing pathogens were identified based on the produced pattern of volatile metabolites. The source of the metabolites is unknown; they can be formed by the pathogens, but they can also be formed by enzymes coming from e.g. the blood of the cow. By inoculating milk of healthy cows with mastitis pathogens, the formation of volatile metabolites by bacteria in milk could be separated from possible other sources of metabolite formation. In general, most metabolites were found in similar concentration in both type of samples. However, some metabolites (mainly ethyl esters of free fatty acids) were not found in inoculated samples, probably due to lack of enzyme activity related to the cows’ blood. It was concluded that volatile bacterial metabolites could be used for pathogen identification, and that the metabolites were mostly formed by the pathogen and are not the result of the mastitis process in the udder.

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Mastitis control

From science to practice

ISBN:: 9789086866496

出版社:: Wageningen Academic

印刷出版日期:: 30 Oct 2008

Subjects
- Life Sciences

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Detection of mastitis pathogens by analysis of volatile metabolites

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Mastitis control

From science to practice

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