Genetic characterisation of inflammatory and immune responses to a bovine Staphylococcus aureus small colony variant and its isogeneic wild type strain

H.N. Atalla; B.A. Mallard

Genetic characterisation of inflammatory and immune responses to a bovine Staphylococcus aureus small colony variant and its isogeneic wild type strain

于Mastitis control

著者：

H.N. Atalla

H.N. Atalla Department of Pathobiology, Ontario Veterinary College, University of Guelph, Canada Corresponding author: bmallard@uoguelph.ca

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B.A. Mallard

B.A. Mallard Department of Pathobiology, Ontario Veterinary College, University of Guelph, Canada Corresponding author: bmallard@uoguelph.ca

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类型:: 章节

Page Range:: 111–117

DOI:: https://doi.org/10.3920/9789086866496_020

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Staphylococcus aureus is one of the most common causes of bovine mastitis and often associated with persistent infection. Persistent infection may relate to the ability of this pathogen to transform into a variant subpopulation termed small colony variant (SCV) that have the potential to survive intracellularly. Internalisation within host cells appears to shield SCV from antibiotics and host responses. S. aureus SCVs have been described in human medicine yet are not well described in cattle. The objective of the current research is to characterise the immune responses associated with bovine S. aureus strains both phenotypically and genetically. A bovine SCV (confirmed by biochemical and genetic analyses) was isolated from the University of Guelph dairy herd. This isolate (SCV Heba3231) had a slow growth rate and prolonged lag phase compared to its parent strain 3231 and Newbould 305. The SCV Heba3231 persisted longer within cultured endothelial cells (BAEC) and a bovine mammary epithelial cell line (MAC-T) without deleterious cell damage compared to its parent strain and Newbould 305. Subsequently, 4 groups of clinically healthy cows, 5 cows/treatment groups were infected intramammary with either SCV Heba3231, its parent strain, a hem B mutant or Newbould 305. Cows infected with the SCVs established mild clinical mastitis compared to those cows infected with the wild type strains. There were also differences in cell-mediated immune responses as assessed by cutaneous delayed-type hypersensitivity among infected cows. Further characterisation of immune response including innate recognition genes, proinflammatory chemokines/cytokines genes, type I and II cytokines and regulatory immune response genes in blood and milk mononuclear cells is ongoing. Analysis of immune response genes should provide insight into the host transcriptomic profiles following interaction with persistent strains of S. aureus mastitis and should provide better understanding of the pathophysiology of bovine mastitis following infection with persistent strains of S. aureus.

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Mastitis control

From science to practice

ISBN:: 9789086866496

出版社:: Wageningen Academic

印刷出版日期:: 30 Oct 2008

Subjects
- Life Sciences

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Genetic characterisation of inflammatory and immune responses to a bovine Staphylococcus aureus small colony variant and its isogeneic wild type strain

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Mastitis control

From science to practice

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