Identification of bacteria associated with mastitis and poor hygiene in bovine farm tank milk

J.L.W. Rademaker

Identification of bacteria associated with mastitis and poor hygiene in bovine farm tank milk

In: Mastitis control

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J.L.W. Rademaker

J.L.W. Rademaker NIZO food research, P.O. Box 20, 6710 BA Ede, the Netherlands Corresponding author: Jan.Rademaker@nizo.nl

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Type:: Chapter

Pages:: 175–181

DOI:: https://doi.org/10.3920/9789086866496_037

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The presence of bacteria associated with poor farm hygiene and bovine mastitis was determined in farm tank milk using DNA-based methods. Random samples of raw farm (bulk) tank milk with high somatic cell counts (HSCC >400,000 cells/ml; n=25), high total bacterial counts (HTBC >100,000 cells/ml; n=25) and low (L)TBC (<10,000 cells/ml; n=42) were analysed. Sixteen samples tested positive with one or more quantitative real-time PCR assays targeting six groups of bacteria. Three positive test results were obtained from samples with HTBC. Thirteen positive tests were obtained from samples with HSCC. SCC is a key indicator of milk quality and is often used as an indirect measure of the overall amount of mastitis that a herd is experiencing. The three samples with HTBC tested PCR-positive for Pseudomonas, Escherichia coli, and Enterococcus. All LTBC samples tested negative. The thirteen PCR positive samples with HSCC all tested positive for Pseudomonas. In addition, these samples tested positive for bacteria that can be associated with mastitis and/or poor hygiene; two samples for Staphylococcus aureus, six samples for Streptococcus uberis, three samples for E. coli, and finally three samples for Enterococcus. In one sample S. uberis and E. coli were found. No samples were tested positive for Streptococcus agalactiae. In conclusion, bacterial flora associated with poor hygiene or mastitis was identified in 16 samples of raw farm tank milk. This supports the suitability of the use of DNA markers in a real-time PCR assay for specific analysis of the microbiological quality of raw milk. The high incidence of Pseudomonas in samples with HSCC indicates that these bacteria may be involved in a (secondary) infection of mastitis.

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Mastitis control

From science to practice

E-Book ISBN:: 9789086866496

Publisher:: Wageningen Academic

Print Publication Date:: 30 Oct 2008

Subjects
- Life Sciences

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Identification of bacteria associated with mastitis and poor hygiene in bovine farm tank milk

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Mastitis control

From science to practice

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