Phenotypic and molecular identification of Streptococcus species isolated from milk of intramammary infected dairy cows in Austria

M. Gonano; P. Winter

Phenotypic and molecular identification of Streptococcus species isolated from milk of intramammary infected dairy cows in Austria

In: Mastitis control

Authors:

M. Gonano

M. Gonano ¹Institute of Milk Hygiene, Milk Technology and Food Science, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria

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P. Winter

P. Winter ¹Institute of Milk Hygiene, Milk Technology and Food Science, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria
²Institute for Veterinary Disease Control, Austrian Agency for Health and Food Safety, Robert Kochgasse 17, 2340 Moedling, Austria Corresponding author: petra.winter@ages.at

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Type:: Chapter

Pages:: 191–198

DOI:: https://doi.org/10.3920/9789086866496_039

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Streptococcal species, especially Streptococcus uberis, are one of the most significant pathogens causing intramammary infections in dairy cattle and responsible for considerable economic loss. The identification of streptococci concentrates on serological Lancefield grouping and analysis of biochemical activity. But it is well known that different species are able to express the same Lancefield group antigen or are not serological groupable. To study the distribution of Streptococcus species we collected 195 gram-positive and catalase negative cocci on bloodagar plates isolated from subclinical and clinical mastitis cases in dairy cows in Austria. After serogrouping and identification to the species level by API 20 Strep kit, the strains were investigated by a multiplex PCR to differentiate seven relevant streptococcal species. Not type-able isolates were transferred to another PCR to examine the enterococci and lactococci strains. Phenotypic and molecular identification was in accordance in 54.9% of the strains. Biochemical typing revealed to following distribution: 37.4% enterococci, 27.7% S. uberis, 10.3% lactococci, 8.2% S. dysgalactiae, 4.6% Aerococcus spp., 3.6% S. agalactiae and 2.6% other streptococci. PCR identification was more precise: 20.5% enterococci, 44.1% S. uberis, 13. % lactococci, 7.2% S. dysgalactiae, 3.1% S. bovis, 1.5% S. agalactiae, 1.0% S. canis, 0.5% S. parauberis and 8.7% non type-able strains. This shows that a reliable biochemical differentiation of Streptococcus species is difficult and fails to detect S. uberis infections. Conventional routine diagnostics have to be improved to give precise information on species level for establishing farm specific mastitis prevention programs.

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Mastitis control

From science to practice

E-Book ISBN:: 9789086866496

Publisher:: Wageningen Academic

Print Publication Date:: 30 Oct 2008

Subjects
- Life Sciences

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Phenotypic and molecular identification of Streptococcus species isolated from milk of intramammary infected dairy cows in Austria

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Mastitis control

From science to practice

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