Alpaca sperm chromatin evaluation using Toluidine Blue

M.I. Carretero; C.C. Arraztoa; C.I. Casaretto; W. Huanca; D.M. Neild; M.S. Giuliano

Alpaca sperm chromatin evaluation using Toluidine Blue

In: Fibre production in South American camelids and other fibre animals

Authors:

M.I. Carretero

M.I. Carretero ¹Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Argentina; ignaciacarretero@fvet.uba.ar

Search for other papers by M.I. Carretero in
Current site
Google Scholar
PubMed

C.C. Arraztoa

C.C. Arraztoa ¹Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Argentina; ignaciacarretero@fvet.uba.ar

Search for other papers by C.C. Arraztoa in
Current site
Google Scholar
PubMed

C.I. Casaretto

C.I. Casaretto ¹Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Argentina; ignaciacarretero@fvet.uba.ar

Search for other papers by C.I. Casaretto in
Current site
Google Scholar
PubMed

W. Huanca

W. Huanca ²Laboratorio de Reproducción Animal, Facultad de Medicina Veterinaria, Universidad Mayor de San Marcos, Perú

Search for other papers by W. Huanca in
Current site
Google Scholar
PubMed

D.M. Neild

D.M. Neild ¹Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Argentina; ignaciacarretero@fvet.uba.ar

Search for other papers by D.M. Neild in
Current site
Google Scholar
PubMed

, and

M.S. Giuliano

M.S. Giuliano ¹Instituto de Investigación y Tecnología en Reproducción Animal (INITRA), Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Argentina; ignaciacarretero@fvet.uba.ar

Search for other papers by M.S. Giuliano in
Current site
Google Scholar
PubMed

Type:: Chapter

Pages:: 141–144

DOI:: https://doi.org/10.3920/9789086867271_019

Purchase instant access (PDF download and unlimited online access):

€42.00€35.00 excl. VAT

Add to Cart

Redeem Access Token

Alpacas are a domestic species of South American camelids (SAC), which are internationally appreciated because they have a high-quality fibre. Toluidine Blue (TB) is a cationic stain that binds to DNA permitting differentiation between sperm heads according to the degree of chromatin decondensation. This technique was used to evaluate sperm chromatin in alpacas, determine chromatin condensation patterns in alpacas and determine if it is possible to use dithiothreitol (DTT) as a positive control for the stain. A total of 15 ejaculates were collected from 9 alpacas using electroejaculation. TB stain was carried out according to Carretero et al. (2009) on smears of raw semen. Spermatozoa were classified according to the degree of chromatin decondensation. Descriptive statistics and analysis of variance for evaluating male differences were performed. Three patterns of staining with TB were observed in alpaca sperm: light blue (negative, without alteration of chromatin condensation), light violet (intermediate, some degree of decondensation), dark blue-violet (positive, high degree of decondensation). The percentages observed were (mean ± SD): TB positive 7.55±5.22, TB intermediate 21.67±6.81 and TB negative 70.78±10.80. In the spermatozoa incubated with 1% DTT, 2 main categories were observed: reacted (positive with TB) and non-reacted (negative with TB). In reacted sperm, 3 sub-categories were observed according to sperm head morphology and presence of vacuoles. Variability between males was observed for positive (P=0.03) but not for intermediate TB stained sperm. It is possible to use TB to evaluate the degree of chromatin decondensation in alpaca spermatozoa, showing the same TB patterns as in other SAC; also 1% DTT can be used as a positive control for this technique.

Citation Info

Save
Cite
Email this content

Share link with colleague or librarian

You can email a link to this page to a colleague or librarian:
Email this content
or copy the link directly:

https://brill.edhh.ma/abstract/book/9789086867271/BP000019.xml
The link was not copied. Your current browser may not support copying via this button.

Link copied successfully

Collapse
Expand

Fibre production in South American camelids and other fibre animals

E-Book ISBN:: 9789086867271

Publisher:: Wageningen Academic

Print Publication Date:: 30 Oct 2011

Subjects
- Life Sciences
  - General
  - Animal & Veterinary

Metrics

	All Time	Past 365 days	Past 30 Days
Abstract Views	153	37	3
Full Text Views	2	0	0
PDF Views & Downloads	2	0	0

African Studies	Education	Media Studies
American Studies	History	Middle East and Islamic Studies
Ancient Near East and Egypt	Human Rights and Humanitarian Law	Musicology
Art History	International Law	Philosophy
Asian Studies	International Relations	Religious Studies
Biblical Studies	Jewish Studies	Slavic and Eurasian Studies
Biology	Languages and Linguistics	Social Sciences
Book History and Cartography	Life Sciences	Theology and World Christianity
Classical Studies	Literature and Cultural Studies

Subjects

Authors

Open Access

Product Information

Company

Contact

Alpaca sperm chromatin evaluation using Toluidine Blue

Citation Info

Share link with colleague or librarian

Fibre production in South American camelids and other fibre animals

Table of Contents

Metrics

Metrics