Revealing pectinâs structure

H.A. Schols; G.-J. Coenen; A.G.J. Voragen

Revealing pectinâs structure

in Pectins and pectinases

Autor:innen:

H.A. Schols

H.A. Schols Wageningen University, Laboratory of Food Chemistry, Bomenweg 2, 6703 HD Wageningen, the Netherlands

Search for other papers by H.A. Schols in
Current site
Google Scholar
PubMed

G.-J. Coenen

G.-J. Coenen Wageningen University, Laboratory of Food Chemistry, Bomenweg 2, 6703 HD Wageningen, the Netherlands

Search for other papers by G.-J. Coenen in
Current site
Google Scholar
PubMed

, und

A.G.J. Voragen

A.G.J. Voragen Wageningen University, Laboratory of Food Chemistry, Bomenweg 2, 6703 HD Wageningen, the Netherlands

Search for other papers by A.G.J. Voragen in
Current site
Google Scholar
PubMed

Art:: Kapitel

Seiten:: 17â34

DOI:: https://doi.org/10.3920/9789086866779_002

Ãber Institut einloggen

Sofortzugang erwerben (PDF-Download und unbegrenzter Online-Zugang):

42,00Â â¬35,00Â â¬Â excl. VAT

Add to Cart

Token einlÃ¶sen

Research towards revealing the structure of the plant cell wall polymer pectin which is being considered as the most complex molecule in nature, lasts already for many decades. Natureâs ability to tailor the chemical structure of pectin molecule according to the plant cell wall and plant tissue needs at that particular moment really complicates such efforts to reveal the structure enormously. As already stated by the past professor Pilnik, the pre-eminent pectin expert in his time, it is more and more realised that âno pectin molecule is alikeâ. As a result of the ongoing search, pectin models proposed in the early nineties are considered not to cover all phenomena observed and being not able to explain all new findings. A new model has been proposed some years ago. This new model further initiated a renewed discussion on the precise chemical structure of the various pectin structural elements, their interconnection and their assembly within the cell wall and has initiated quite some new research. Part of the efforts made in the Laboratory of Food Chemistry in Wageningen within the PhD research of Coenen (2007) in order to reveal the connection points between the different pectic subunits and the establishment/development of novel analytical techniques needed for such an investigation are summarised in this paper. Oligosaccharides, obtained by controlled acid hydrolysis of apple pectin, were characterized using optimized LC-MS and NMR approaches. The structures established for these oligosaccharides indicated that in apple pectin both homogalacturonan (HG) and xylogalacturonan (XGA) were covalently linked to rhamnogalacturonan I (RGI). A newly developed method coupled capillary electrophoresis (CE) on-line to mass spectrometry (MS) and allowed identification of compounds present in CE electropherograms of complex apple XGA and RGI digests. By combining Î²-eliminative breakdown with CE-LIF and CE-UVMSn analysis, detailed information on the neutral sugar side chain structure and distribution was obtained of some enzyme resistant structural elements of soy pectin. Using selective degradation by Î²-elimination, it was demonstrated that arabinans containing a rudimentary RGI backbone fragment play an important role within the complement fixing activity of white cabbage (Brassica oleracea) pectin. Based on the results obtained in this research and data reported in literature, a pectin model is proposed which positions HG both in-line and as a side chain of RGI. In addition, new insights in the position of XGA with respect to HG and RGI were obtained. A further increase of our knowledge on pectin and polysaccharide structures can be achieved by combining the âcombined approachesâ described in this contribution with other chemical and novel enzymatic degradation methods and by implementing these methods more quantitatively.

Zitierungsangaben

Save
Cite
Inhalt per Mail versenden

Link teilen

Sie kÃ¶nnen einen Link zu dieser Seite per E-Mail senden:
Inhalt per Mail versenden
oder den Link direkt kopieren:

https://brill.edhh.ma/abstract/book/9789086866779/BP000002.xml
Der Link wurde nicht kopiert. Ihr aktueller Browser unterstÃ¼tzt das Kopieren Ã¼ber diese SchaltflÃ¤che mÃ¶glicherweise nicht.

Link wurde kopiert

Reduzieren
Erweitern
Nach oben

Pectins and pectinases

ISBN:: 9789086866779

Verleger:: Wageningen Academic

Print-Publikationsdatum:: 30 Oct 2009

Fachgebiete
- Biowissenschaften
  - Allgemein
  - ErnÃ¤hrung und Gesundheit

Kennzahlen

	Insgesamt	Letzte 365 Tage	In den letzten 30 Tagen
Aufrufe von Kurzbeschreibungen	348	113	7
Gesamttextansichten	5	1	0
PDF-Downloads	5	2	0

Afrikanistik	Internationale Beziehungen	Nahost- und Islamwissenschaften
Altorientalistik und Ägyptologie	Internationales Recht	Pädagogik
Amerikanistik	Judaistik	Philosophie
Asienwissenschaften	Klassische Altertumswissenschaft	Religionswissenschaft
Bibelauslegung	Kunstgeschichte	Slawistik und Eurasienkunde
Biologie	Literatur- und Kulturwissenschaften	Sozialwissenschaften
Biowissenschaften	Medienwissenschaft	Sprachen und Linguistik
Buchgeschichte und Kartographie	Menschenrechte und humanitäres Völkerrecht	Theologie und Christentum
Geschichte	Musikwissenschaft

Fachgebiete

Autor:innen

Open Access

Produkt-Informationen

Ãber uns

Kontakt

Revealing pectinâs structure

Zitierungsangaben

Link teilen

Pectins and pectinases

Inhaltsverzeichnis

Kennzahlen

Kennzahlen

Revealing pectinâs structure