Structural elucidation of cell wall polysaccharides from okra (Abelmoschus esculentus (L.) Moench)

N. Sengkhamparn; R. Verhoef; E.J. Bakx; H.A. Schols; T. Sajjaanantakul; A.G.J. Voragen

Structural elucidation of cell wall polysaccharides from okra (Abelmoschus esculentus (L.) Moench)

In: Pectins and pectinases

Authors:

N. Sengkhamparn

N. Sengkhamparn ¹Laboratory of Food Chemistry, Department of Agrotechnology and Food Sciences, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, the Netherlands
²Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, Ladyao, Jatujak, Bangkok 10900, Thailand

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R. Verhoef

R. Verhoef ¹Laboratory of Food Chemistry, Department of Agrotechnology and Food Sciences, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, the Netherlands

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E.J. Bakx

E.J. Bakx ²Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, Ladyao, Jatujak, Bangkok 10900, Thailand

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H.A. Schols

H.A. Schols ¹Laboratory of Food Chemistry, Department of Agrotechnology and Food Sciences, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, the Netherlands

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T. Sajjaanantakul

T. Sajjaanantakul ²Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, Ladyao, Jatujak, Bangkok 10900, Thailand

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A.G.J. Voragen

A.G.J. Voragen ¹Laboratory of Food Chemistry, Department of Agrotechnology and Food Sciences, Wageningen University, P.O. Box 8129, 6700 EV Wageningen, the Netherlands

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Type:: Chapter

Pages:: 49–56

DOI:: https://doi.org/10.3920/9789086866779_004

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Polysaccharides making up the walls of okra tissue where isolated by a sequential extraction procedure. This yielded different polysaccharide fractions. The Hot Buffer Soluble Solids (HBSS) fraction contained a regular structure which was predominately composed of highly branched rhamnogalacturonan (RG) I elements with short galactan side chains (1-2 galactose units). The degree of acetylation of this rhamnogalacturonan was relatively high. NMR studies of partially hydrolysed HBSS using rhamnogalacturonan hydrolase revealed that the rhamnose residues were O-acetylated and/or substituted with a terminal alpha-linked galactose moiety. The CHelating agent Soluble Solids (CHSS) fraction contained mainly relatively high methyl esterified homogalacturonan segments carrying minor quantities of RGI with galactan side chains (2-3 galactose units) which were slightly longer than found for HBSS RGI. The CHSS fraction contained arabinan and arabinogalactan (AG) type II structures as neutral side chains of the rhamnogalacturonan structural element. The Diluted Alkali Soluble Solids (DASS) fraction was characterized by high amounts of rhamnose, galactose, galacturonic acid, next to significant levels of arabinose, indicating that this RGI carried arabinans, AG type I and AG type II structures as neutral side chains. The hemicellulose fraction from the okra AIS represented mainly xyloglucan and xylan as identified in the Concentrated Alkali Soluble Solids (CASS) fraction.

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Pectins and pectinases

E-Book ISBN:: 9789086866779

Publisher:: Wageningen Academic

Print Publication Date:: 30 Oct 2009

Subjects
- Life Sciences
  - General
  - Food & Health

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Structural elucidation of cell wall polysaccharides from okra (Abelmoschus esculentus (L.) Moench)

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