Limited phytate hydrolysis by endogenous phytase of sesame seed during germination

K. Żyła; T. Fortuna; M. Mika; M. Czubak

Limited phytate hydrolysis by endogenous phytase of sesame seed during germination

In: Recent advances of research in antinutritional factors in legume seeds and oilseeds

Authors:

K. Żyła

K. Żyła ¹Department of Food Biotechnology, University of Agriculture in Kraków, 29-Listopada Ave 46, 31-425 Kraków, Poland

Search for other papers by K. Żyła in
Current site
Google Scholar
PubMed

T. Fortuna

T. Fortuna ²Department of Food Analysis and Food Quality Assessment, University of Agriculture in Kraków, ul. Balicka 122, 30-149 Kraków, Poland

Search for other papers by T. Fortuna in
Current site
Google Scholar
PubMed

M. Mika

M. Mika ¹Department of Food Biotechnology, University of Agriculture in Kraków, 29-Listopada Ave 46, 31-425 Kraków, Poland

Search for other papers by M. Mika in
Current site
Google Scholar
PubMed

, and

M. Czubak

M. Czubak ¹Department of Food Biotechnology, University of Agriculture in Kraków, 29-Listopada Ave 46, 31-425 Kraków, Poland

Search for other papers by M. Czubak in
Current site
Google Scholar
PubMed

Type:: Chapter

Pages:: 347–350

DOI:: https://doi.org/10.3920/978-90-8686-524-6_056

Purchase instant access (PDF download and unlimited online access):

€42.00€35.00 excl. VAT

Add to Cart

Redeem Access Token

It is well know that germination improves the nutritional quality of oilseeds. The objective of this work was to determine changes in phytase activity, in the chemical composition and the in vitro nutrient digestibility of germinated sesame seed (Sesamum indicum L.). The seed was germinated for 12, 24, 36, 48 and 60 h, at 25, 30, 35, 40, and 45 °C. Phytase activity was measured colourimetrically following cellulase and xylanase assisted extraction. Inositol phosphates were determined by HPLC and in vitro digestibility was measured by peptic and pancreatic digestion of samples.

The sesame phytase activity increased from 1.4 FTU/g in raw seeds to 5.0 FTU/g after 36 h of germination. The optimal germination temperature was 30 °C at pH 5,5. As a result of germination the sesame seeds fat and carbohydrates content decreased from 47 to 40 %, and from 6.05 to 2.03 %, respectively. However protein level was not altered (27 vs. 28 %). Total phosphorus, calcium and magnesium were unchanged, and the inorganic seed phosphorus increased from 0.6 to 1.4 mg/g. During germination IP6 concentration of IP6 decreased slightly (from 38.2 to 36.4 μM/g ddb¹), the decrease in IP5 concentration was not significant, and only minor amounts of IP4 were produced. In the in vitro digestion test seed germination improved the digestibility of phosphorus, calcium, magnesium and carbohydrates, but did not affect protein digestibility.

Citation Info

Save
Cite
Email this content

Share link with colleague or librarian

You can email a link to this page to a colleague or librarian:
Email this content
or copy the link directly:

https://brill.edhh.ma/configurable/content/book$002f9789086865246$002fBP000056.xml
The link was not copied. Your current browser may not support copying via this button.

Link copied successfully

Collapse
Expand

Recent advances of research in antinutritional factors in legume seeds and oilseeds

Proceedings of the fourth international workshop on antinutritional factors in legume seeds and oilseeds

Series: EAAP Scientific Series, Volume: 110

Cover Recent advances of research in antinutritional factors in legume seeds and oilseeds

E-Book ISBN:: 9789086865246

Publisher:: Wageningen Academic

Print Publication Date:: 10 Oct 2004

Subjects
- Life Sciences
  - General
  - Animal & Veterinary

Metrics

	All Time	Past 365 days	Past 30 Days
Abstract Views	85	42	17
Full Text Views	0	0	0
PDF Views & Downloads	0	0	0

African Studies	Education	Media Studies
American Studies	History	Middle East and Islamic Studies
Ancient Near East and Egypt	Human Rights and Humanitarian Law	Musicology
Art History	International Law	Philosophy
Asian Studies	International Relations	Religious Studies
Biblical Studies	Jewish Studies	Slavic and Eurasian Studies
Biology	Languages and Linguistics	Social Sciences
Book History and Cartography	Life Sciences	Theology and World Christianity
Classical Studies	Literature and Cultural Studies

Subjects

Authors

Open Access

Product Information

Company

Contact

Limited phytate hydrolysis by endogenous phytase of sesame seed during germination

Citation Info

Share link with colleague or librarian

Recent advances of research in antinutritional factors in legume seeds and oilseeds

Proceedings of the fourth international workshop on antinutritional factors in legume seeds and oilseeds

Table of Contents

Metrics

Metrics