Hydrodynamic properties of isolated pectin domains: a way to figure out pectin macromolecular structure?

M.-C. Ralet; J.-F. Thibault

Hydrodynamic properties of isolated pectin domains: a way to figure out pectin macromolecular structure?

in Pectins and pectinases

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M.-C. Ralet

M.-C. Ralet INRA, UR1268 Biopolymères Interactions Assemblages, B.P. 71627, 44316 Nantes cedex 03, France

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J.-F. Thibault

J.-F. Thibault INRA, UR1268 Biopolymères Interactions Assemblages, B.P. 71627, 44316 Nantes cedex 03, France

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Art:: Kapitel

Seiten:: 35–48

DOI:: https://doi.org/10.3920/9789086866779_003

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Pectins are extremely complex polysaccharides that can be viewed as multiblock cobiopolymers. Although the fine structure of the different pectic blocks is now quite well known, the way in which they are combined into a macromolecular structure is still a matter of debate. Large differences in pectins quality and quantity exist between different cell types, at different stages of cellular development and even within the thickness of a given wall. A way to understand how the different structural elements are connected to one another to form variable macromolecular structures is to isolate and identify the different connection points. Another, and complementary, way is to specifically isolate the different pectic domains in order to study their hydrodynamic properties. Six monocotyledonous and dicotyledonous species were selected and pectins were extracted from cell wall materials using a four-step sequential extraction scheme. The isolation and characterization of pectin structural elements showed that structural discrepancies of pectins differing in HG/RGI balance could be due to a variable amount of HG domains decorating an RGI backbone. Our results also support the hypothesis of a hyper-variability of RGI backbone length. However, the structural complexity and heterogeneity of pectins make it very difficult to appraise the relationship between pectin fine structure and hydrodynamic properties, especially when the compared pectins are derived from different plant species and obtained with different extractants. The Arabidopsis pectin mutant quasimodo2 was thereby studied. By specifically isolating HG blocks, qua2 appeared to be specifically deficient in HG, with no change in the amount of RGI blocks. Moreover, the remaining HGs had maintained the same size as those in the wild type. The possible macromolecular build up of pectins is discussed in the light of the presented results.

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Pectins and pectinases

ISBN:: 9789086866779

Verleger:: Wageningen Academic

Print-Publikationsdatum:: 30 Oct 2009

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Hydrodynamic properties of isolated pectin domains: a way to figure out pectin macromolecular structure?

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Pectins and pectinases

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