Experiments in automatic ovulation prediction in dairy cows

T. Mottram; M. Velasco-Garcia

Experiments in automatic ovulation prediction in dairy cows

于Performance recording of animals - State of the art, 2002

著者：

T. Mottram

T. Mottram Silsoe Research Institute, Wrest Park, Silsoe, Bedford, MK45 4HS, United Kingdom

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M. Velasco-Garcia

M. Velasco-Garcia Silsoe Research Institute, Wrest Park, Silsoe, Bedford, MK45 4HS, United Kingdom

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类型:: 章节

Page Range:: 327–332

DOI:: https://doi.org/10.3920/9789086865116_051

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An automated biosensor system for detecting pregnancy and predicting ovulation in dairy cows was built and tested. The system automates the sampling of milk and the analysis of the milk by a deployment of a screen printed carbon electrode biosensor for progesterone. Test and calibration benchmarks to validate performance have been developed. The biosensor system measured concentrations of 3-20 ng/ml progesterone in fresh milk with an R²=0.965 in the process detecting pregnancy in two out of two test cows and the ovulation cycles of five cows.

The system was constructed based on a screen printed carbon electrode using a proprietary ink printed onto a pvc substrate, on which antibody layers to bind to progesterone in solution were deposited. Milk was applied to the sensor by a system of pumps and valves to allow the manual steps to operate the assay to be computer controlled. This was interfaced to a milking machine using a sampler loaned from a commercial collaborator. The whole system was built into a box that could be interfaced to any milking machine. A series of tests were developed to ensure that each stage of the process operated successfully. The final system will integrate the biosensor with an automatic milk sampling device and a herd management database, operating with minimal servicing.

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Performance recording of animals - State of the art, 2002

Proceedings of the 33rd Biennial Session of ICAR, Interlaken, Switzerland

丛编： EAAP Scientific Series, 卷： 107

Cover Performance recording of animals - State of the art, 2002

ISBN:: 9789086865116

出版社:: Wageningen Academic

印刷出版日期:: 10 Oct 2003

Subjects
- Life Sciences
  - General
  - Animal & Veterinary

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Experiments in automatic ovulation prediction in dairy cows

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Performance recording of animals - State of the art, 2002

Proceedings of the 33rd Biennial Session of ICAR, Interlaken, Switzerland

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