Effect of Vernonia amygdalina leaf meal on growth performance, intestinal mucosa activity, digestive enzymes, absorption capacity, and immunity in broiler chickens
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Laboratoire de Recherche Avicole et de Zoo-Economie, Faculté des Sciences Agronomiques, Université d’Abomey-Calavi, 00229 Cotonou, Bénin.
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Animal Physiology Department, Federal University of Agriculture Abeokuta, 00234 Abeokuta, Nigeria.
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Gut health is multifaceted and is largely influenced by the rearing environment and the diet. The use of phytochemicals rich in phenolics and flavonoids can improve the digestive health of chickens and lead to better growth performance. The aim of this study was to examine the effects of dietary Vernonia amygdalina leaf meal (VALM) on growth performance, digestive enzyme activities, absorption function, organ weights and immunity of broilers. Two hundred and forty, one-day-old male Cobb 500 broiler chicks were randomly divided into four groups: an unsupplemented control and VA-1, VA-3 and VA-5 receiving VALM incorporation at concentrations of 1, 3 and 5 g/kg, respectively. Each treatment had six replicates of 10 chickens. On d 42, six chickens per replicate were isolated and euthanised. Digesta from the jejunal segments (10 cm) was collected for analysis of the digestive enzymes. The remaining digesta was then washed out with ice-cold phosphate-buffered saline before the jejunal segments (10 cm) were opened longitudinally to collect the mucosa by scraping. For the preparation of the homogenate, intestinal mucosa samples were homogenised with 154 mmol/l of ice-cold sodium chloride solution and centrifuged at 4 °C for 900 s. To determine immunoglobulins, glutathione and D-xylose, the supernatant was extracted and stored at -20 °C. Supplementation with VALM did not significantly influence the relative weights of organs in the different treatments. However, VALM at 3 g/kg caused a significant increase in amylase and trypsin concentration (P<0.05). Immunoglobulin A and intestinal secretory immunoglobulin G concentrations were significantly improved (P<0.05) in the birds fed 3 g/kg VALM. This supported the premise that 3 g/kg VALM in feed can improve gastric immunity status and digestive enzyme secretion.
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Effect of Vernonia amygdalina leaf meal on growth performance, intestinal mucosa activity, digestive enzymes, absorption capacity, and immunity in broiler chickens
In: Journal of Applied Animal NutritionSearch for other papers by B.M. Tokofai in
Current site
Google Scholar
PubMed
Laboratoire de Recherche Avicole et de Zoo-Economie, Faculté des Sciences Agronomiques, Université d’Abomey-Calavi, 00229 Cotonou, Bénin.
Search for other papers by B.M. Orounladji in
Current site
Google Scholar
PubMed
Search for other papers by K. Idoh in
Current site
Google Scholar
PubMed
Animal Physiology Department, Federal University of Agriculture Abeokuta, 00234 Abeokuta, Nigeria.
Search for other papers by O.E. Oke in
Current site
Google Scholar
PubMed
Search for other papers by A. Agbonon in
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PubMed
Gut health is multifaceted and is largely influenced by the rearing environment and the diet. The use of phytochemicals rich in phenolics and flavonoids can improve the digestive health of chickens and lead to better growth performance. The aim of this study was to examine the effects of dietary Vernonia amygdalina leaf meal (VALM) on growth performance, digestive enzyme activities, absorption function, organ weights and immunity of broilers. Two hundred and forty, one-day-old male Cobb 500 broiler chicks were randomly divided into four groups: an unsupplemented control and VA-1, VA-3 and VA-5 receiving VALM incorporation at concentrations of 1, 3 and 5 g/kg, respectively. Each treatment had six replicates of 10 chickens. On d 42, six chickens per replicate were isolated and euthanised. Digesta from the jejunal segments (10 cm) was collected for analysis of the digestive enzymes. The remaining digesta was then washed out with ice-cold phosphate-buffered saline before the jejunal segments (10 cm) were opened longitudinally to collect the mucosa by scraping. For the preparation of the homogenate, intestinal mucosa samples were homogenised with 154 mmol/l of ice-cold sodium chloride solution and centrifuged at 4 °C for 900 s. To determine immunoglobulins, glutathione and D-xylose, the supernatant was extracted and stored at -20 °C. Supplementation with VALM did not significantly influence the relative weights of organs in the different treatments. However, VALM at 3 g/kg caused a significant increase in amylase and trypsin concentration (P<0.05). Immunoglobulin A and intestinal secretory immunoglobulin G concentrations were significantly improved (P<0.05) in the birds fed 3 g/kg VALM. This supported the premise that 3 g/kg VALM in feed can improve gastric immunity status and digestive enzyme secretion.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 0 | 0 | 0 |
| Full Text Views | 706 | 278 | 46 |
| PDF Views & Downloads | 727 | 241 | 65 |
