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Detached leafin vitro model for masked mycotoxin biosynthesis and subsequent analysis of unknown conjugates

In: World Mycotoxin Journal
Authors:
M. De Boevre Department of Bioanalysis, Laboratory of Food Analysis, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium

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A. Vanheule

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K. Audenaert

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B. Bekaert Department of Applied Bioscience Engineering, Building C, Ghent University, Valentin Vaerwyckweg 1, 9000 Ghent, Belgium

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J. Diana Di Mavungu Department of Bioanalysis, Laboratory of Food Analysis, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium

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S. Werbrouck

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G. Haesaert

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S. De Saeger Department of Bioanalysis, Laboratory of Food Analysis, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium

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The manuscript details the development of anin vitro model plant system using detached leaves because there is a need for biosynthetic methods for the production and isolation of masked mycotoxins. This detached leafin vitro model was firstly applied to deoxynivalenol with satisfying results. The biosynthesis of deoxynivalenol-3-glucoside was confirmed using its respective commercially available reference standard. Secondly, the detached leafin vitro model was applied to T-2 toxin. Mono- and tri-glucoside derivatives of T-2 toxin and HT-2 toxin, T-2-(3)-glucoside, T-2-(3)-triglucoside and HT-2-(3)-glucoside were identified and characterised using Orbitrap high-resolution mass spectrometry. This is the first report on a triglucoside of T-2 toxin. The discovery of new masked forms implies the importance of the development of analytical methods for their detection, the constitution of toxicity studies, and proving the relevance of their presence in the food and feed chain.

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