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Detached leafin vitro model for masked mycotoxin biosynthesis and subsequent analysis of unknown conjugates
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The manuscript details the development of anin vitro model plant system using detached leaves because there is a need for biosynthetic methods for the production and isolation of masked mycotoxins. This detached leafin vitro model was firstly applied to deoxynivalenol with satisfying results. The biosynthesis of deoxynivalenol-3-glucoside was confirmed using its respective commercially available reference standard. Secondly, the detached leafin vitro model was applied to T-2 toxin. Mono- and tri-glucoside derivatives of T-2 toxin and HT-2 toxin, T-2-(3)-glucoside, T-2-(3)-triglucoside and HT-2-(3)-glucoside were identified and characterised using Orbitrap high-resolution mass spectrometry. This is the first report on a triglucoside of T-2 toxin. The discovery of new masked forms implies the importance of the development of analytical methods for their detection, the constitution of toxicity studies, and proving the relevance of their presence in the food and feed chain.
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| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 177 | 75 | 7 |
| Gesamttextansichten | 19 | 0 | 0 |
| PDF-Downloads | 8 | 0 | 0 |
Detached leafin vitro model for masked mycotoxin biosynthesis and subsequent analysis of unknown conjugates
in World Mycotoxin JournalSearch for other papers by M. De Boevre in
Current site
Google Scholar
PubMed
Search for other papers by A. Vanheule in
Current site
Google Scholar
PubMed
Search for other papers by K. Audenaert in
Current site
Google Scholar
PubMed
Search for other papers by B. Bekaert in
Current site
Google Scholar
PubMed
Search for other papers by J. Diana Di Mavungu in
Current site
Google Scholar
PubMed
Search for other papers by S. Werbrouck in
Current site
Google Scholar
PubMed
Search for other papers by G. Haesaert in
Current site
Google Scholar
PubMed
Search for other papers by S. De Saeger in
Current site
Google Scholar
PubMed
The manuscript details the development of anin vitro model plant system using detached leaves because there is a need for biosynthetic methods for the production and isolation of masked mycotoxins. This detached leafin vitro model was firstly applied to deoxynivalenol with satisfying results. The biosynthesis of deoxynivalenol-3-glucoside was confirmed using its respective commercially available reference standard. Secondly, the detached leafin vitro model was applied to T-2 toxin. Mono- and tri-glucoside derivatives of T-2 toxin and HT-2 toxin, T-2-(3)-glucoside, T-2-(3)-triglucoside and HT-2-(3)-glucoside were identified and characterised using Orbitrap high-resolution mass spectrometry. This is the first report on a triglucoside of T-2 toxin. The discovery of new masked forms implies the importance of the development of analytical methods for their detection, the constitution of toxicity studies, and proving the relevance of their presence in the food and feed chain.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 177 | 75 | 7 |
| Gesamttextansichten | 19 | 0 | 0 |
| PDF-Downloads | 8 | 0 | 0 |
