Mushrooms versus fungi: natural compounds from Lentinula edodes inhibit aflatoxin biosynthesis by Aspergillus parasiticus
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Although the strategies routinely adopted to limit production of carcinogenic aflatoxins by Aspergillus parasiticus and Aspergillus flavus in foods and feeds can be quite effective, they are often neither environmentally friendly, nor non-toxic for end users. Polysaccharides and glycoproteins, particularly β-glucans from the basidiomycete Lentinula edodes, already known for their health-promoting effects on animals and humans, have previously demonstrated the ability to inhibit aflatoxin biosynthesis by stimulating the antioxidant defences of the toxigenic fungus. Here the results of a study regarding the influence on polysaccharide production by L. edodes of oxidative stress induced using paraquat (PQ) treatment are reported. Paraquat 0.5 and 1 mM resulted in an enhancement of the expression of the β-glucan synthase gene Lefks1 and a consequent stimulating effect (about 30-35%) on β-glucans production. Moreover, oxidative-stress (PQ) induced polysaccharides have a higher aflatoxin inhibiting capacity in two different strains of A. parasiticus in comparison with non-induced polysaccharides. This more efficient inhibition might be explained by a higher content of β-glucans because 1H-NMR analysis revealed no obvious qualitative differences between PQ-induced and non-induced polysaccharides. The results obtained show promise for improving the quantity and efficiency of L. edodes extracts in order to achieve enhanced control over aflatoxin biosynthesis in foods and feeds using health-promoting and environmentally-friendly compounds.
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Fanelli, C., Tasca, V., Ricelli, A., Reverberi, M., Zjalic, S., Finotti, E. and Fabbri, A.A., 2000. Inhibiting effect of medicinal mushroom Lentinus edodes (Berk.) Sing (Agaricomycetideae) on aflatoxin production by Aspergillus parasiticus Speare. International Journal of Medicinal Mushrooms 2: 229-236.
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Reverberi, M., Fabbri, A.A., Zjalic, S., Ricelli, A., Punelli, F. and Fanelli, C., 2005. Antioxidant enzymes stimulation in Aspergillus parasiticus by Lentinula edodes inhibits aflatoxin production. Applied Microbiology and Biotechnology 69: 207-215.
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Tolaini, V., Zjalic, S., Reverberi, M., Fanelli, C., Fabbri, A.A. and Ricelli, A., 2010. Lentinula edodes enhances the biocontrol activity of Cryptococcus laurentii against Penicillium expansum contamination and patulin production in apple fruits. International Journal of Food Microbiology 138: 243-249.
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Zjalic, S., Fabbri, A.A., Reverberi, M., Ricelli, A., Punelli, F. and Fanelli, C., 2007. Use of Trametes versicolor in control of aflatoxins produced by Aspergillus parasiticus. International Journal of Medicinal Mushrooms 9: 236-237.
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Zjalic, S., Reverberi, M., Ricelli, A., Granito, V.M., Fanelli, C. and Fabbri, A.A., 2006. Trametes versicolor: a possible tool for aflatoxin control. International Journal of Food Microbiology 107: 243-249.
'Trametes versicolor: a possible tool for aflatoxin control ' () 107 International Journal of Food Microbiology : 243 -249 .
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Mushrooms versus fungi: natural compounds from Lentinula edodes inhibit aflatoxin biosynthesis by Aspergillus parasiticus
In: World Mycotoxin JournalSearch for other papers by M. Reverberi in
Current site
Google Scholar
PubMed
Search for other papers by S. Zjalic in
Current site
Google Scholar
PubMed
Search for other papers by A. Ricelli in
Current site
Google Scholar
PubMed
Search for other papers by C. Di Meo in
Current site
Google Scholar
PubMed
Search for other papers by M. Scarpari in
Current site
Google Scholar
PubMed
Search for other papers by C. Fanelli in
Current site
Google Scholar
PubMed
Search for other papers by A. Fabbri in
Current site
Google Scholar
PubMed
Although the strategies routinely adopted to limit production of carcinogenic aflatoxins by Aspergillus parasiticus and Aspergillus flavus in foods and feeds can be quite effective, they are often neither environmentally friendly, nor non-toxic for end users. Polysaccharides and glycoproteins, particularly β-glucans from the basidiomycete Lentinula edodes, already known for their health-promoting effects on animals and humans, have previously demonstrated the ability to inhibit aflatoxin biosynthesis by stimulating the antioxidant defences of the toxigenic fungus. Here the results of a study regarding the influence on polysaccharide production by L. edodes of oxidative stress induced using paraquat (PQ) treatment are reported. Paraquat 0.5 and 1 mM resulted in an enhancement of the expression of the β-glucan synthase gene Lefks1 and a consequent stimulating effect (about 30-35%) on β-glucans production. Moreover, oxidative-stress (PQ) induced polysaccharides have a higher aflatoxin inhibiting capacity in two different strains of A. parasiticus in comparison with non-induced polysaccharides. This more efficient inhibition might be explained by a higher content of β-glucans because 1H-NMR analysis revealed no obvious qualitative differences between PQ-induced and non-induced polysaccharides. The results obtained show promise for improving the quantity and efficiency of L. edodes extracts in order to achieve enhanced control over aflatoxin biosynthesis in foods and feeds using health-promoting and environmentally-friendly compounds.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 232 | 72 | 4 |
| Full Text Views | 19 | 0 | 0 |
| PDF Views & Downloads | 12 | 0 | 0 |
