Prevalence of Aspergillus species on selected Colombian animal feedstuffs and ability of Aspergillus section Flavi to produce aflatoxins
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A total of 57 samples of feedstuffs commonly used for animal nutrition in Colombia (maize, soybean, sorghum, cottonseed meal, sunflower seed meal, wheat middlings and rice) were analysed for Aspergillus contamination. Aspergillus fungi were identified at species level and their ability to produce aflatoxins was determined by highperformance liquid chromatography. A total of 31 of the feedstuffs analysed (54.4%) were found to contain Aspergillus spp. The most contaminated substrate was maize (100%) followed by cottonseed meal (80%), sorghum (60%) and wheat middlings (60%). Soybean showed lower levels of contamination (10%). No Aspergillus spp. could be isolated from rice or sunflower seed meal. Total Aspergillus strains isolated were 50, with 28 belonging to section Flavi (56%), 17 to section Nigri (34%), 4 to section Circumdati (8%) and 1 to section Fumigati (2%). Among section Flavi, 17 isolates were identified as A. flavus, seven as A. parasiticus, two as A. oryzae and two as A. tamarii. Production of aflatoxins by Aspergillus section Flavi was screened by liquid chromatography. About three quarters of the A. flavus strains (76.5%) produced aflatoxin B1 (0.2 to 240.4 µg/g) and aflatoxin B2 (0.2 to 1.6 µg/g), while all A. parasiticus strains produced the four naturally occurring aflatoxins (aflatoxin B1 from 0.6 to 83.5 µg/g, aflatoxin B2 from 0.3 to 4.8 µg/g, aflatoxin G1 from 0.4 to 19.3 µg/g and aflatoxin G2 from 0.1 to 1.0 µg/g). This is the first study demonstrating the presence of highly toxigenic Aspergillus fungi in Colombian animal feedstuffs.
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Cary, J.W. and Ehrlich, K.C., 2006. Aflatoxigenicity in Aspergillus: molecular genetics, phylogenetic relationships and evolutionary implications. Mycopathologia 162: 167-177.
'Aflatoxigenicity in Aspergillus: molecular genetics, phylogenetic relationships and evolutionary implications ' () 162 Mycopathologia : 167 -177 .
-
Céspedes, A.E. and Diaz, G.J., 1997. Analysis of aflatoxins in poultry and pig feeds and feedstuffs used in Colombia. Journal of AOAC International 80: 1215-1219.
'Analysis of aflatoxins in poultry and pig feeds and feedstuffs used in Colombia ' () 80 Journal of AOAC International : 1215 -1219 .
-
Cotty P.J., 1997. Aflatoxin-producing potential of communities of Aspergillus section Flavi from cotton producing areas of the United States. Mycological Research 101: 698-704.
-
Davis, N.D., Iyer, S.K. and Diener, U.L., 1987. Improved method of screening for aflatoxin with a coconut agar medium. Applied and Environmental Microbiology 53: 1593-1595.
'Improved method of screening for aflatoxin with a coconut agar medium ' () 53 Applied and Environmental Microbiology : 1593 -1595 .
-
Diaz G.J., Perilla, N.S. and Rojas, Y., 2001. Occurrence of aflatoxins in selected Colombian foods. Mycotoxin Research 17: 15-20.
-
Hara S., Fennell, D.I. and Hesseltine, C.W., 1974. Aflatoxin–producing strains of Aspergillus flavus detected by fluorescence of agar medium under ultraviolet light. Applied Microbiology 27: 1118-1123.
'Aflatoxin–producing strains of Aspergillus flavus detected by fluorescence of agar medium under ultraviolet light ' () 27 Applied Microbiology : 1118 -1123 .
-
Hocking, A., 1997. Toxigenic Aspergillus Species. In: Doyle, M.P., Beuchat, L.R. and Montville, T.J. (Eds.) Food microbiology: fundamentals and frontiers. ASM Press, Washington D.C., USA, pp. 393-405.
-
Horn, B.W. and Dorner, J.W., 1999. Regional differences in production of aflatoxin B1 and cyclopiazonic acid by soil isolates of Aspergillus flavus along a transect within the United States. Applied and Environmental Microbiology 65: 1444-1449.
'Regional differences in production of aflatoxin B1 and cyclopiazonic acid by soil isolates of Aspergillus flavus along a transect within the United States ' () 65 Applied and Environmental Microbiology : 1444 -1449 .
-
Huang, X., Dorner, J.W. and Chu, F.S., 1994. Production of aflatoxin and cyclopiazonic acid by various Aspergilli: an ELISA analysis. Mycotoxin Research 10: 101-106.
-
Hussein, H.S. and Brasel, J.M., 2001. Toxicity, metabolism, and impact of mycotoxins on humans and animals. Toxicology 167: 101-134.
'Toxicity, metabolism, and impact of mycotoxins on humans and animals ' () 167 Toxicology : 101 -134 .
-
Klich, M.A., 2000. Identification of common Aspergillus species. United States Department of Agriculture, Agricultural Research Service, Southern Regional Research Center. New Orleans, LA, USA.
-
Kurtzman, C.P., Horn, B.W. and Hesseltine, C.W., 1987. Aspergillus nomius, a new aflatoxin-producing species related to Aspergillus flavus and Aspergillus tamarii. Antonie Van Leeuwenhoek 53: 147-158.
Aspergillus nomius , ().
-
Lisker, N., Michaeli, R. and Frank, Z.R., 1993. Mycotoxigenic potential of Aspergillus flavus strains isolated from groundnuts growing in Israel. Mycopathologia 122: 177-183.
'Mycotoxigenic potential of Aspergillus flavus strains isolated from groundnuts growing in Israel ' () 122 Mycopathologia : 177 -183 .
-
McLean M. and Dutton, M.F., 1995. Cellular interactions and metabolism of aflatoxin: An update. Pharmacology and Therapeutics 65: 163-192.
'Cellular interactions and metabolism of aflatoxin: An update ' () 65 Pharmacology and Therapeutics : 163 -192 .
-
Pacin, A.M., Gonzalez, H.H., Etcheverry, M., Resnik, S.L., Vivas, L. and Espin, S., 2003. Fungi associated with food and feed commodities from Ecuador. Mycopathologia 156: 87-92.
'Fungi associated with food and feed commodities from Ecuador ' () 156 Mycopathologia : 87 -92 .
-
Pier, A.C., 1992. Major biological consequences of aflatoxicosis in animal production. Journal of Animal Science 70: 3964-3967.
'Major biological consequences of aflatoxicosis in animal production ' () 70 Journal of Animal Science : 3964 -3967 .
-
Pitt, J.I., 2006. Fungal ecology and the occurrence of mycotoxins. In: Njapau, H., Trujillo, S., Van Egmond, H.P. and Park, D.L. (eds.) Mycotoxins and phycotoxins. Wageningen Academic Publishers, Wageningen, The Netherlands, pp. 33-41.
-
Pitt, J.I., Hocking, A.D. and Glenn, D.R., 1983. An improved medium for the detection of Aspergillus flavus and A. parasiticus. Journal of Applied Bacteriology 54: 109-114.
'An improved medium for the detection of Aspergillus flavus and ' () 54 A. parasiticus. Journal of Applied Bacteriology : 109 -114 .
-
Rodríguez, D.B. and Sabino, M., 2002. Mycotoxin research in Brazil: The last decade in review. Brazilian Journal of Microbiology 33: 1-11.
-
Schroeder, H.W. and Boller, R.A., 1973. Aflatoxin production of species and strains of the Aspergillus flavus group isolated from field crops. Applied Microbiology 25: 885-889.
'Aflatoxin production of species and strains of the Aspergillus flavus group isolated from field crops ' () 25 Applied Microbiology : 885 -889 .
-
World Health Organization (WHO), 2002. IARC monographs on the evaluation of carcinogenic risk to humans. Vol. 82. Some traditional herbal medicine, some mycotoxins, naphthalene and styrene. IARC Press, Lyon, France.
-
Xie, G. and West, T.P., 2006. Citric acid production by Aspergillus niger on wet corn distillers grains. Letters in Applied Microbiology 43: 269-273.
'Citric acid production by Aspergillus niger on wet corn distillers grains ' () 43 Letters in Applied Microbiology : 269 -273 .
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Prevalence of Aspergillus species on selected Colombian animal feedstuffs and ability of Aspergillus section Flavi to produce aflatoxins
In: World Mycotoxin JournalSearch for other papers by G. Diaz in
Current site
Google Scholar
PubMed
Search for other papers by M. Lozano in
Current site
Google Scholar
PubMed
Search for other papers by A. Acuña in
Current site
Google Scholar
PubMed
A total of 57 samples of feedstuffs commonly used for animal nutrition in Colombia (maize, soybean, sorghum, cottonseed meal, sunflower seed meal, wheat middlings and rice) were analysed for Aspergillus contamination. Aspergillus fungi were identified at species level and their ability to produce aflatoxins was determined by highperformance liquid chromatography. A total of 31 of the feedstuffs analysed (54.4%) were found to contain Aspergillus spp. The most contaminated substrate was maize (100%) followed by cottonseed meal (80%), sorghum (60%) and wheat middlings (60%). Soybean showed lower levels of contamination (10%). No Aspergillus spp. could be isolated from rice or sunflower seed meal. Total Aspergillus strains isolated were 50, with 28 belonging to section Flavi (56%), 17 to section Nigri (34%), 4 to section Circumdati (8%) and 1 to section Fumigati (2%). Among section Flavi, 17 isolates were identified as A. flavus, seven as A. parasiticus, two as A. oryzae and two as A. tamarii. Production of aflatoxins by Aspergillus section Flavi was screened by liquid chromatography. About three quarters of the A. flavus strains (76.5%) produced aflatoxin B1 (0.2 to 240.4 µg/g) and aflatoxin B2 (0.2 to 1.6 µg/g), while all A. parasiticus strains produced the four naturally occurring aflatoxins (aflatoxin B1 from 0.6 to 83.5 µg/g, aflatoxin B2 from 0.3 to 4.8 µg/g, aflatoxin G1 from 0.4 to 19.3 µg/g and aflatoxin G2 from 0.1 to 1.0 µg/g). This is the first study demonstrating the presence of highly toxigenic Aspergillus fungi in Colombian animal feedstuffs.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 160 | 65 | 6 |
| Full Text Views | 19 | 0 | 0 |
| PDF Views & Downloads | 4 | 0 | 0 |
