Effects of resistance training combined with creatine hydrochloride or creatine monohydrate supplementation on oxidative stress-antioxidant markers in trained women: a double-blind randomized placebo-controlled trial
In: Comparative Exercise PhysiologySearch for other papers by S.S. Dadvand in
Current site
Google Scholar
Department of Exercise Physiology, Faculty of Sport Sciences, Ferdowsi University of Mashhad, Mashhad 9177948974, Iran
Search for other papers by H. Arazi in
Current site
Google Scholar
Abstract
This study evaluated the effects of resistance training (RT) combined with creatine hydrochloride (CrHCL) and creatine monohydrate (CrM) supplementation on oxidative stress-antioxidant markers in trained women. In this study, 48 young trained women were selected and randomly assigned to four groups via a double-blind method: Group 1: RT + Creatine hydrochloride (Cr-HCL) supplementation (n = 12), Group 2: RT + Creatine monohydrate-loading phase (CrM-LP) supplementation (n = 12), Group 3: RT + CrM-without loading phase (CrM-WLP) supplementation (n = 12), and Group 4: RT + Placebo (PL) supplementation (n = 12). The RT program was performed for eight weeks with three sessions per week. Blood samples were obtained before and after training to evaluate serum levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) activity. Statistical analyses were performed using the Mixed ANOVA and Bonferroni post-hoc tests at a significance level of 0.05. Following the intervention, the RT + Cr-HCL, RT + CrM-LP, and RT + CrM-WLP supplementation groups indicated significant decreases and increases in the blood levels of MDA and SOD, respectively, compared to the RT + PL supplementation group (
The study was conducted based on the Declaration of Helsinki and was approved by the ethics committee of the Sport Sciences Research Institute (IR.SSRC.REC.1399.138).
Purchase
Buy instant access (PDF download and unlimited online access):
Institutional Login
Log in with Open Athens, Shibboleth, or your institutional credentials
Personal login
Log in with your brill.com account
-
Alraddadi, E.A., Lillico, R., Vennerstrom, J.L., Lakowski, T.M. and Miller, D.W., 2018. Absolute oral bioavailability of creatine monohydrate in rats: debunking a myth. Pharmaceutics 10: 31. https://doi.org/10.3390/pharmaceutics10010031
-
Araújo, M.B., Moura, L.P., Junior, R.C.V., Junior, M.C., Dalia, R.A., Sponton, A.C., Ribeiro, C. and Mello, M.A.R., 2013. Creatine supplementation and oxidative stress in rat liver. Journal of the International Society of Sports Nutrition 10: 54. https://doi.org/10.1186/1550-2783-10-54
-
Bagchi, D., 2018. Nutrition and enhanced sports performance: muscle building, endurance, and strength. Academic Press.
-
Balsom, P.D., Söderlund, K. and Ekblom, B., 1994. Creatine in humans with special reference to creatine supplementation. Sports Medicine 18: 268-280. https://doi.org/10.2165/00007256-199418040-00005
-
Barcelos, R.P., Stefanello, S.T., Mauriz, J.L., Gonzalez-Gallego, J. and Soares, F.A.A., 2016. Creatine and the liver: metabolism and possible interactions. Mini-Reviews in Medical Chemistry 16: 12-18. https://doi.org/10.2174/1389557515666150722102613
-
Beck, T.W., Housh, T.J., Johnson, G.O., Coburn, J.W., Malek, M.H. and Cramer, J.T., 2007. Effects of a drink containing creatine, amino acids, and protein combined with ten weeks of resistance training on body composition, strength, and anaerobic performance. Journal of Strength and Conditioning Research 21: 100-104. https://doi.org/10.1519/r-18685.1
-
Bonilla, D.A., Kreider, R.B., Stout, J.R., Forero, D.A., Kerksick, C.M., Roberts, M.D. and Rawson, E.S., 2021. Metabolic basis of creatine in health and disease: a bioinformatics-assisted review. Nutrients 13: 1238. https://doi.org/10.3390/nu13041238
-
Brosnan, M.E. and Brosnan, J.T., 2016. The role of dietary creatine. Amino Acids 48: 1785-1791. https://doi.org/10.1007/s00726-016-2188-1
-
Burton, G.J. and Jauniaux, E., 2011. Oxidative stress. Baillière’s Best Practice & Research Clinical Obstetrics & Gynaecology 25: 287-299. https://doi.org/10.1016/j.bpobgyn.2010.10.016
-
Butts, J., Jacobs, B. and Silvis, M., 2018. Creatine use in sports. Sports Health 10: 31-34. https://doi.org/10.1177/1941738117737248
-
Çakir-Atabek, H., Demir, S., PinarbaSili, R.D. and Gündüz, N., 2010. Effects of different resistance training intensity on indices of oxidative stress. Journal of Strength and Conditioning Research 24: 2491-2497. https://doi.org/10.1519/JSC.0b013e3181ddb111
-
Chandrasekaran, A., Idelchik, M.D.P.S. and Melendez, J.A., 2017. Redox control of senescence and age-related disease. Redox Biology 11: 91-102. https://doi.org/10.1016/j.redox.2016.11.005
-
Clarke, H., Kim, D.H., Meza, C.A., Ormsbee, M.J. and Hickner, R.C., 2020. The evolving applications of creatine supplementation: could creatine improve vascular health?. Nutrients 12: 2834. https://doi.org/10.3390/nu12092834
-
Cohen, J., 2013. Statistical power analysis for the behavioral sciences. Routledge. https://doi.org/10.4324/9780203771587
-
Cooper, R., Naclerio, F., Allgrove, J. and Jimenez, A., 2012. Creatine supplementation with specific view to exercise/sports performance: an update. Journal of the International Society of Sports Nutrition 9: 1-11. https://doi.org/10.1186/1550-2783-9-33
-
Dash, A.K., Miller, D.W., Huai-Yan, H., Carnazzo, J. and Stout, J.R., 2001. Evaluation of creatine transport using Caco-2 monolayers as an in vitro model for intestinal absorption. Journal of Pharmaceutical Sciences 90: 1593-1598. https://doi.org/10.1002/jps.1109
-
de França, E., Avelar, B., Yoshioka, C., Santana, J.O., Madureira, D., Rocha, L.Y., Zocoler, C.A., Rossi, F.E., Lira, F.S., Rodrigues, B. and Caperuto, É.C., 2015. Creatine HCl and creatine monohydrate improve strength but only creatine HCl induced changes on body composition in recreational weightlifters. Food and Nutrition Sciences 6: 1624. https://doi.org/10.4236/fns.2015.617167
-
Deldicque, L., Décombaz, J., Zbinden Foncea, H., Vuichoud, J., Poortmans, J.R. and Francaux, M., 2008. Kinetics of creatine ingested as a food ingredient. European Journal of Applied Physiology 102: 133-143. https://doi.org/10.1007/s00421-007-0558-9
-
Deminice, R., Rosa, F.T., Franco, G.S., Jordao, A.A. and de Freitas, E.C., 2013. Effects of creatine supplementation on oxidative stress and inflammatory markers after repeated-sprint exercise in humans. Nutrition 29: 1127-1132. https://doi.org/10.1016/j.nut.2013.03.003
-
Di Meo, S. and Venditti, P., 2001. Mitochondria in exercise-induced oxidative stress. Neurosignals 10: 125-140. https://doi.org/10.1159/000046880
-
Eley, H.L., Russell, S.T., Baxter, J.H., Mukerji, P. and Tisdale, M.J., 2007. Signaling pathways initiated by β-hydroxy-β-methylbutyrate to attenuate the depression of protein synthesis in skeletal muscle in response to cachectic stimuli. American Journal of Physiology: Endocrinology and Metabolism 293: E923-E931. https://doi.org/10.1152/ajpendo.00314.2007
-
Faul, F., Erdfelder, E., Lang, A.G. and Buchner, A., 2007. G*Power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behavior Research Methods 39: 175-191. https://doi.org/10.3758/BF03193146
-
Fazio, C., Elder, C.L. and Harris, M.M., 2022. Efficacy of alternative forms of creatine supplementation on improving performance and body composition in healthy subjects: a systematic review. Journal of Strength and Conditioning Research. https://doi.org/10.1519/JSC.0000000000003873
-
Fimognari, C., Sestili, P., Lenzi, M., Cantelli-Forti, G. and Hrelia, P., 2009. Protective effect of creatine against RNA damage. Mutation Research 670: 59-67. https://doi.org/10.1016/j.mrfmmm.2009.07.005
-
Fisher-Wellman, K. and Bloomer, R.J., 2009. Acute exercise and oxidative stress: a 30 year history. Dynamic Medicine 8: 1-25. https://doi.org/10.1186/1476-5918-8-1
-
Fleck, S.J. and Kraemer, W., 2014. Designing resistance training programs. 4th ed. Human Kinetics.
-
Greenwood, M., Cooke, M.B., Ziegenfuss, T., Kalman, D.S. and Antonio, J., 2015. Nutritional supplements in sports and exercise. Springer.
-
Grune, T., Reinheckel, T. and Davies, K.J., 1997. Degradation of oxidized proteins in mammalian cells. The FASEB Journal 11: 526-534. https://doi.org/10.1096/fasebj.11.7.9212076
-
Gufford, B.T., Sriraghavan, K., Miller, N.J., Miller, D.W., Gu, X., Vennerstrom, J.L. and Robinson, D.H., 2010. Physicochemical characterization of creatine N-methylguanidinium salts. Journal of Dietary Supplements 7: 240-252. https://doi.org/10.3109/19390211.2010.491507
-
Guidi, C., Potenza, L., Sestili, P., Martinelli, C., Guescini, M., Stocchi, L., Zeppa, S., Polidori, E., Annibalini, G. and Stocchi, V., 2008. Differential effect of creatine on oxidatively-injured mitochondrial and nuclear DNA. Biochimica Et Biophysica Acta G, General Subjects 1780: 16-26. https://doi.org/10.1016/j.bbagen.2007.09.018
-
He, F., Li, J., Liu, Z., Chuang, C.C., Yang, W. and Zuo, L., 2016. Redox mechanism of reactive oxygen species in exercise. Frontiers in Physiology 7: 486. https://doi.org/10.3389/fphys.2016.00486
-
Hermann, T. and Westhof, E., 2000. Rational drug design and high-throughput techniques for RNA targets. Combinatorial Chemistry & High Throughput Screening 3: 219-234. https://doi.org/10.2174/1386207003331652
-
Ives, S.J., Norton, C., Miller, V., Minicucci, O., Robinson, J., O’Brien, G., Escudero, D., Paul, M., Sheridan, C., Curran, K. and Rose, K., 2017. Multi-modal exercise training and protein-pacing enhances physical performance adaptations independent of growth hormone and BDNF but may be dependent on IGF-1 in exercise-trained men. Growth Hormone & IGF Research 32: 60-70. https://doi.org/10.1016/j.ghir.2016.10.002
-
Jäger, R., Purpura, M., Shao, A., Inoue, T. and Kreider, R.B., 2011. Analysis of the efficacy, safety, and regulatory status of novel forms of creatine. Amino Acids 40: 1369-1383. https://doi.org/10.1007/s00726-011-0874-6
-
Lambertucci, R.H., Levada-Pires, A.C., Rossoni, L.V., Curi, R. and Pithon-Curi, T.C., 2007. Effects of aerobic exercise training on antioxidant enzyme activities and mRNA levels in soleus muscle from young and aged rats. Mechanisms of Ageing and Development 128: 267-275. https://doi.org/10.1016/j.mad.2006.12.006
-
Laughlin, M.H., Simpson, T., Sexton, W.L., Brown, O.R., Smith, J.K. and Korthuis, R.J., 1990. Skeletal muscle oxidative capacity, antioxidant enzymes, and exercise training. Journal of Applied Physiology 68: 2337-2343. https://doi.org/10.1152/jappl.1990.68.6.2337
-
Leeuwenburgh, C., Fiebig, R., Chandwaney, R.J.L.L. and Ji, L.L., 1994. Aging and exercise training in skeletal muscle: responses of glutathione and antioxidant enzyme systems. American Journal of Physiology Regulatory, Integrative and Comparative Physiology 267: R439-R445. https://doi.org/10.1152/ajpregu.1994.267.2.R439
-
Liguori, G., 2020. Medicine ACoS. ACSM’s guidelines for exercise testing and prescription. Lippincott Williams & Wilkins.
-
Liguori, I., Russo, G., Curcio, F., Bulli, G., Aran, L., Della-Morte, D., Gargiulo, G., Testa, G., Cacciatore, F., Bonaduce, D. and Abete, P., 2018. Oxidative stress, aging, and diseases. Clinical Interventions in Aging: 757-772. https://doi.org/10.2147/CIA.S158513
-
Louis, M., Van Beneden, R., Dehoux, M., Thissen, J.P. and Francaux, M., 2004. Creatine increases IGF-I and myogenic regulatory factor mRNA in C2C12 cells. FEBS Letters 557: 243-247. https://doi.org/10.1016/S0014-5793(03)01504-7
-
McDonough, D., 2017. Oral creatine hydrochloride supplementation: acute effects on submaximal, intermittent bouts of bench press and vertical jump exercises. https://doi.org/10.18122/B2KX4Q
-
Nakajima, S., Kamohara, S., Nakano, M. and Ohno, M., 2006. Antioxidant supplementation decreases the amount of urinary 8-OHdG excretion induced by a single bout of exercise. Japanese Journal of Physical Fitness and Sports Medicine 55(Supplement): S251-S256. https://doi.org/10.7600/jspfsm.55.S251
-
Percário, S., Domingues, S.P.D.T., Teixeira, L.F.M., Vieira, J.L.F., de Vasconcelos, F., Ciarrocchi, D.M., Almeida, E.D. and Conte, M., 2012. Effects of creatine supplementation on oxidative stress profile of athletes. Journal of the International Society of Sports Nutrition 9: 1-8. https://doi.org/10.1186/1550-2783-9-56
-
Persky, A.M., Brazeau, G.A. and Hochhaus, G., 2003. Pharmacokinetics of the dietary supplement creatine. Clinical Pharmacokinetics 42: 557-574. https://doi.org/10.2165/00003088-200342060-00005
-
Proske, U. and Morgan, D.L., 2001. Muscle damage from eccentric exercise: mechanism, mechanical signs, adaptation and clinical applications. Journal of Physiology 537: 333-345. https://doi.org/10.1111/j.1469-7793.2001.00333.x
-
Rahimi, R., 2011. Creatine supplementation decreases oxidative DNA damage and lipid peroxidation induced by a single bout of resistance exercise. Journal of Strength and Conditioning Research 25: 3448-3455. https://doi.org/10.1519/JSC.0b013e3182162f2b
-
Reddy, P.H., 2008. Mitochondrial medicine for aging and neurodegenerative diseases. Neuromolecular Medicine 10: 291-315. https://doi.org/10.1007/s12017-008-8044-z
-
Reid, M.B., 2001. Invited review: redox modulation of skeletal muscle contraction: what we know and what we don’t. Journal of Applied Physiology 90: 724-731. https://doi.org/10.1152/jappl.2001.90.2.724
-
Rudrapal, M., Khairnar, S.J., Khan, J., Dukhyil, A.B., Ansari, M.A., Alomary, M.N., Alshabrmi, F.M., Palai, S., Deb, P.K. and Devi, R., 2022. Dietary polyphenols and their role in oxidative stress-induced human diseases: insights into protective effects, antioxidant potentials and mechanism (s) of action. Front Pharmacol 13: 806470. https://doi.org/10.3389/fphar.2022.806470
-
Sen, C., Packer, L. and Hänninen, O., 2000. Exercise and oxygen radical production by muscle. In: Handbook of oxidants and antioxidants in exercise 57.
-
Sestili, P., Martinelli, C., Bravi, G., Piccoli, G., Curci, R., Battistelli, M., Falcieri, E., Agostini, D., Gioacchini, A.M. and Stocchi, V., 2006. Creatine supplementation affords cytoprotection in oxidatively injured cultured mammalian cells via direct antioxidant activity. Free Radical Biology and Medicine 40: 837-849. https://doi.org/10.1016/j.freeradbiomed.2005.10.035
-
Silva, L.A., Tromm, C.B., Da Rosa, G., Bom, K., Luciano, T.F., Tuon, T., De Souza, C.T. and Pinho, R.A., 2013. Creatine supplementation does not decrease oxidative stress and inflammation in skeletal muscle after eccentric exercise. Journal of Sports Sciences 31: 1164-1176. https://doi.org/10.1080/02640414.2013.773403
-
Stefani, G.P., Nunes, R.B., Dornelles, A.Z., Alves, J.P., Piva, M.O., Domenico, M.D., Rhoden, C.R. and Lago, P.D., 2014b. Effects of creatine supplementation associated with resistance training on oxidative stress in different tissues of rats. Journal of the International Society of Sports Nutrition 11: 11. https://doi.org/10.1186/1550-2783-11-11
-
Stoppani, J., 2021. Supplement Breakdown: creatine HCL. JS Stoppani Blog. Westlake Village, CA, USA 24.
-
Sword, D.O., 2012. Exercise as a management strategy for the overweight and obese: where does resistance exercise fit in? Strength & Conditioning Journal 34: 47-55. https://doi.org/10.1519/SSC.0b013e31826d9403
-
Tayebi, M. and Arazi, H., 2020. Is creatine hydrochloride better than creatine monohydrate for the improvement of physical performance and hormonal changes in young trained men? Science & Sports 35: e135-e141. https://doi.org/10.1016/j.scispo.2019.07.013
-
Tuckfield, C., 2015. First use of creatine hydrochloride in premanifest Huntington disease. Medical Journal of Australia 202: 378-380. https://doi.org/10.5694/mja14.01070
-
Wax, B., Kerksick, C.M., Jagim, A.R., Mayo, J.J., Lyons, B.C. and Kreider, R.B., 2021. Creatine for exercise and sports performance, with recovery considerations for healthy populations. Nutrients 13: 1915. https://doi.org/10.3390/nu13061915
-
Wilson, J.M., Lowery, R.P., Joy, J.M., Andersen, J.C., Wilson, S.M., Stout, J.R., Duncan, N., Fuller, J.C., Baier, S.M., Naimo, M.A. and Rathmacher, J., 2014. The effects of 12 weeks of beta-hydroxy-beta-methylbutyrate free acid supplementation on muscle mass, strength, and power in resistance-trained individuals: a randomized, double-blind, placebo-controlled study. European Journal of Applied Physiology 114: 1217-1227. https://doi.org/10.1007/s00421-014-2854-5
-
Zhao, X., Bey, E.A., Wientjes, F.B. and Cathcart, M.K., 2002. Cytosolic phospholipase A2 (cPLA2) regulation of human monocyte NADPH oxidase activity: cPLA2 affects translocation but not phosphorylation of p67 phox and p47 phox. The Journal of Biological Chemistry 277: 25385-25392. https://doi.org/10.1074/jbc.M203630200
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 775 | 594 | 26 |
| Full Text Views | 33 | 29 | 0 |
| PDF Views & Downloads | 58 | 49 | 0 |
Effects of resistance training combined with creatine hydrochloride or creatine monohydrate supplementation on oxidative stress-antioxidant markers in trained women: a double-blind randomized placebo-controlled trial
In: Comparative Exercise PhysiologySearch for other papers by S.S. Dadvand in
Current site
Google Scholar
PubMed
Department of Exercise Physiology, Faculty of Sport Sciences, Ferdowsi University of Mashhad, Mashhad 9177948974, Iran
Search for other papers by H. Arazi in
Current site
Google Scholar
PubMed
Abstract
This study evaluated the effects of resistance training (RT) combined with creatine hydrochloride (CrHCL) and creatine monohydrate (CrM) supplementation on oxidative stress-antioxidant markers in trained women. In this study, 48 young trained women were selected and randomly assigned to four groups via a double-blind method: Group 1: RT + Creatine hydrochloride (Cr-HCL) supplementation (n = 12), Group 2: RT + Creatine monohydrate-loading phase (CrM-LP) supplementation (n = 12), Group 3: RT + CrM-without loading phase (CrM-WLP) supplementation (n = 12), and Group 4: RT + Placebo (PL) supplementation (n = 12). The RT program was performed for eight weeks with three sessions per week. Blood samples were obtained before and after training to evaluate serum levels of 8-hydroxy-2-deoxyguanosine (8-OHdG), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPX) and catalase (CAT) activity. Statistical analyses were performed using the Mixed ANOVA and Bonferroni post-hoc tests at a significance level of 0.05. Following the intervention, the RT + Cr-HCL, RT + CrM-LP, and RT + CrM-WLP supplementation groups indicated significant decreases and increases in the blood levels of MDA and SOD, respectively, compared to the RT + PL supplementation group (
The study was conducted based on the Declaration of Helsinki and was approved by the ethics committee of the Sport Sciences Research Institute (IR.SSRC.REC.1399.138).
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 775 | 594 | 26 |
| Full Text Views | 33 | 29 | 0 |
| PDF Views & Downloads | 58 | 49 | 0 |
