Maize is currently the most important crop in China. A major concern in maize production is maize ear rot caused byFusarium spp., which results in yield losses, reduction of seed quality and the accumulation of mycotoxins in the harvested grains. To identify the importance of the differentFusarium species in maize infection, we performed a comprehensive survey on 9,000 asymptomatic and randomly collected maize kernels. Seeds were collected from 12 different provinces covering all major maize growing areas in China and included five maize varieties. In total 1,022Fusarium isolates were retrieved that were identified based on morphological characteristics, by species specific diagnostic PCRs and by EF1-α gene sequencing. Eight different species were identified:Fusarium verticillioides (75.34%),Fusarium graminearum (8.32%),Fusarium proliferatum (7.14%),Fusarium subglutinans (4.11%),Fusarium meridionale (1.57%),Fusarium oxysporum (1.37%),Fusarium semitectum (1.17%), andFusarium asiaticum (0.98%). The distribution ofFusarium species was found to be different in different regions with the largest diversity observed in Hubei province, where all eightFusarium species were isolated. Genetic chemotyping within theF. graminearum species complex indicated that all of the 85F. graminearum isolates showed the 15-acetyldeoxynivalenol chemotype, whereas allF. asiaticum (n=10) andF. meridionale (n=16) isolates had the nivalenol chemotype even when isolated from the same maize field. To our knowledge this is the largest collection ofFusarium isolates from maize and further exploitations of this collection are discussed.
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Maize is currently the most important crop in China. A major concern in maize production is maize ear rot caused byFusarium spp., which results in yield losses, reduction of seed quality and the accumulation of mycotoxins in the harvested grains. To identify the importance of the differentFusarium species in maize infection, we performed a comprehensive survey on 9,000 asymptomatic and randomly collected maize kernels. Seeds were collected from 12 different provinces covering all major maize growing areas in China and included five maize varieties. In total 1,022Fusarium isolates were retrieved that were identified based on morphological characteristics, by species specific diagnostic PCRs and by EF1-α gene sequencing. Eight different species were identified:Fusarium verticillioides (75.34%),Fusarium graminearum (8.32%),Fusarium proliferatum (7.14%),Fusarium subglutinans (4.11%),Fusarium meridionale (1.57%),Fusarium oxysporum (1.37%),Fusarium semitectum (1.17%), andFusarium asiaticum (0.98%). The distribution ofFusarium species was found to be different in different regions with the largest diversity observed in Hubei province, where all eightFusarium species were isolated. Genetic chemotyping within theF. graminearum species complex indicated that all of the 85F. graminearum isolates showed the 15-acetyldeoxynivalenol chemotype, whereas allF. asiaticum (n=10) andF. meridionale (n=16) isolates had the nivalenol chemotype even when isolated from the same maize field. To our knowledge this is the largest collection ofFusarium isolates from maize and further exploitations of this collection are discussed.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 0 | 0 | 0 |
| Full Text Views | 493 | 146 | 13 |
| PDF Views & Downloads | 232 | 78 | 6 |