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Current PCR-based methods for the detection of mycotoxigenic fungi in complex food and feed matrices
in World Mycotoxin JournalKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China P.R.
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China P.R.
Search for other papers by H. Ur Rahman in
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Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China P.R.
Laboratory of Quality & Safety Risk Assessment for Oilseeds Products, Wuhan, Ministry of Agriculture, Wuhan 430062, China P.R.
Search for other papers by X. Yue in
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Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China P.R.
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China P.R.
National Reference Laboratory for Agricultural Testing (Biotoxin), Wuhan 430062, China P.R.
Search for other papers by Q. Yu in
Current site
Google Scholar
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China P.R.
Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China P.R.
Search for other papers by W. Zhang in
Current site
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Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China P.R.
Laboratory of Quality & Safety Risk Assessment for Oilseeds Products, Wuhan, Ministry of Agriculture, Wuhan 430062, China P.R.
Search for other papers by Q. Zhang in
Current site
Google Scholar
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China P.R.
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China P.R.
Laboratory of Quality & Safety Risk Assessment for Oilseeds Products, Wuhan, Ministry of Agriculture, Wuhan 430062, China P.R.
National Reference Laboratory for Agricultural Testing (Biotoxin), Wuhan 430062, China P.R.
Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China P.R.
Search for other papers by P. Li in
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Mycotoxins are toxic secondary fungal metabolites produced by certain types of filamentous fungi, such asAspergillus, Fusarium, andPenicillium spp. Mycotoxigenic fungi and their produced mycotoxins are considered to be an important issue in food and feed safety due to their toxic effects like carcinogenicity, immunosuppression, neurotoxicity, nephrotoxicity, and hepatotoxicity on humans and animals. To boost the safety level of food and feedstuff, detection and identification of toxins are essential at critical control points across food and feed chains. Zero-tolerance policies by the European Union and other organizations about the extreme low level of tolerance of mycotoxins contamination in food and feed matrices have led to an increasing interest to design more sensitive, specific, rapid, cost-effective, and safer to use mycotoxigenic fungi detection technologies. Hence, many mycotoxigenic fungi detection technologies have been applied to measure and control toxins contamination in food and feed substrates. PCR-based mycotoxigenic fungi detection technologies, such as conventional PCR, real-time PCR, nested PCR, reverse transcriptase (RT)-PCR, loop-mediated isothermal amplification (LAMP),in situ PCR, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR DGGE), co-operational PCR, multiplex PCR, DNA arrays, magnetic capture-hybridization (MCH)-PCR and restriction fragment length polymorphism (RFLP), would contribute to our understanding about different mycotoxigenic fungi detection approaches and will enhance our capability about mycotoxigenic fungi identification, isolation and characterization at critical control points across food and feed chains. We have assessed the principles, results, the limit of detection, and application of these PCR-based detection technologies to alleviate mycotoxins contamination problem in complex food and feed substrates. The potential application of these detection technologies can reduce mycotoxins in complex food and feed matrices.
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Current PCR-based methods for the detection of mycotoxigenic fungi in complex food and feed matrices
in World Mycotoxin JournalKey Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China P.R.
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China P.R.
Search for other papers by H. Ur Rahman in
Current site
Google Scholar
PubMed
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China P.R.
Laboratory of Quality & Safety Risk Assessment for Oilseeds Products, Wuhan, Ministry of Agriculture, Wuhan 430062, China P.R.
Search for other papers by X. Yue in
Current site
Google Scholar
PubMed
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China P.R.
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China P.R.
National Reference Laboratory for Agricultural Testing (Biotoxin), Wuhan 430062, China P.R.
Search for other papers by Q. Yu in
Current site
Google Scholar
PubMed
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China P.R.
Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China P.R.
Search for other papers by W. Zhang in
Current site
Google Scholar
PubMed
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China P.R.
Laboratory of Quality & Safety Risk Assessment for Oilseeds Products, Wuhan, Ministry of Agriculture, Wuhan 430062, China P.R.
Search for other papers by Q. Zhang in
Current site
Google Scholar
PubMed
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China P.R.
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China P.R.
Laboratory of Quality & Safety Risk Assessment for Oilseeds Products, Wuhan, Ministry of Agriculture, Wuhan 430062, China P.R.
National Reference Laboratory for Agricultural Testing (Biotoxin), Wuhan 430062, China P.R.
Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan 430062, China P.R.
Search for other papers by P. Li in
Current site
Google Scholar
PubMed
Mycotoxins are toxic secondary fungal metabolites produced by certain types of filamentous fungi, such asAspergillus, Fusarium, andPenicillium spp. Mycotoxigenic fungi and their produced mycotoxins are considered to be an important issue in food and feed safety due to their toxic effects like carcinogenicity, immunosuppression, neurotoxicity, nephrotoxicity, and hepatotoxicity on humans and animals. To boost the safety level of food and feedstuff, detection and identification of toxins are essential at critical control points across food and feed chains. Zero-tolerance policies by the European Union and other organizations about the extreme low level of tolerance of mycotoxins contamination in food and feed matrices have led to an increasing interest to design more sensitive, specific, rapid, cost-effective, and safer to use mycotoxigenic fungi detection technologies. Hence, many mycotoxigenic fungi detection technologies have been applied to measure and control toxins contamination in food and feed substrates. PCR-based mycotoxigenic fungi detection technologies, such as conventional PCR, real-time PCR, nested PCR, reverse transcriptase (RT)-PCR, loop-mediated isothermal amplification (LAMP),in situ PCR, polymerase chain reaction-denaturing gradient gel electrophoresis (PCR DGGE), co-operational PCR, multiplex PCR, DNA arrays, magnetic capture-hybridization (MCH)-PCR and restriction fragment length polymorphism (RFLP), would contribute to our understanding about different mycotoxigenic fungi detection approaches and will enhance our capability about mycotoxigenic fungi identification, isolation and characterization at critical control points across food and feed chains. We have assessed the principles, results, the limit of detection, and application of these PCR-based detection technologies to alleviate mycotoxins contamination problem in complex food and feed substrates. The potential application of these detection technologies can reduce mycotoxins in complex food and feed matrices.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 0 | 0 | 0 |
| Gesamttextansichten | 639 | 186 | 19 |
| PDF-Downloads | 379 | 153 | 7 |
