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Cross-reactivity of commercial and non-commercial deoxynivalenol-antibodies to emerging trichothecenes and common deoxynivalenol-derivatives
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Immunoassay based techniques are an important and fast option for the detection and quantification of mycotoxins. They are frequently used as on-site screening tools in grain elevators, storage and production facilities. However, accurate quantification may be hampered by the co-recognition of structurally related metabolites by the used antibodies. Therefore, it is crucial to assess their cross-reactivity to avoid misinterpretation of the results. Several immunoassays for the determination of deoxynivalenol (DON) are commercially available. Recently, novel trichothecene mycotoxins with structures similar to DON, the NX-toxins (NX-2, NX-3 and NX-4), were discovered, which can potentially co-occur with DON in cereals. So far, no data about the cross-reactivity of those toxins with DON-antibodies are available. The aim of this study was to assess the cross-reactivities of NX-toxins and some other DON-related metabolites with DON-antibodies in buffer solutions. Six commercially available enzyme-linked immunosorbent assays (ELISAs) and two previously developed DON-antibodies (Mab#1 and Mab#22) were tested. Cross-reactivity with NX-metabolites was not observed for any of the ELISA-kits nor Mab#22, whereas Mab#1 reacted moderately against NX-3 and NX-4 (cross-reactivity based on a molar basis of 14 and 30%, respectively). Modifications at position C-3 (3-acetyl-DON and DON-3-glucoside) led to moderate or high cross-reactivity with Mab#22 and the commercial ELISA-kits, whereas these compounds were not recognised by Mab#1. Similar to NX-metabolites, 15-acetyl-DON interacted only weakly with Mab#22 and the commercial ELISA-kits, but strongly with Mab#1. The results demonstrate the importance of proper antibody characterisation. If NX-metabolites prove to be widely distributed and reach significant levels, the development of specific antibodies targeting these novel metabolites might become necessary.
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Aamot, H.U., Hofgaard, I.S., Brodal, G., Elen, O., Jestoi, M. and Klemsdal, S.S., 2012. Evaluation of rapid test kits for quantification of deoxynivalenol in naturally contaminated oats and wheat. World Mycotoxin Journal 5: 339-350.
'Evaluation of rapid test kits for quantification of deoxynivalenol in naturally contaminated oats and wheat ' () 5 World Mycotoxin Journal : 339 -350.
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Berthiller, F., Cramer, B., Iha, M.H., Krska, R., Lattanzio, V.M.T., MacDonald, S., Malone, R.J., Maragos, C., Solfrizzo, M., Stranska-Zachariasova, M., Stroka, J. and Tittlemier, S.A., 2018. Developments in mycotoxin analysis: an update for 2016-2017. World Mycotoxin Journal 11: 5-31.
'Developments in mycotoxin analysis: an update for 2016-2017 ' () 11 World Mycotoxin Journal : 5 -31.
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'Policy on characterization of antibodies used in immunochemical methods of analysis for mycotoxins and phycotoxins ' () 15 Journal of AOAC International : 4718 -871.
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'Relative aggressiveness and production of 3- or 15-acetyl deoxynivalenol and deoxynivalenol byFusarium graminearum in spring wheat ' () 32 Canadian Journal of Plant Pathology : 146 -152.
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'The geographic distribution and complex evolutionary history of the NX-2 trichothecene chemotype fromFusarium graminearum ' () 95 Fungal Genetics and Biology : 39 -48.
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Kelly, A.C., Clear, R.M., O’Donnell K., McCormick, S., Turkington, T.K., Tekauz, A., Gilbert, J., Kistler, H.C., Busman, M. and Ward, T.J., 2015. Diversity ofFusarium head blight populations and trichothecene toxin types reveals regional differences in pathogen composition and temporal dynamics. Fungal Genetics and Biology 82: 22-31.
'Diversity ofFusarium head blight populations and trichothecene toxin types reveals regional differences in pathogen composition and temporal dynamics ' () 82 Fungal Genetics and Biology : 22 -31.
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'Production of group specific monoclonal antibody to aflatoxins and its application to enzyme-linked immunosorbent assay ' () 27 Toxicology Research : 125 -131.
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Li, P., Zhang, Q., Zhang, W., Zhang, J., Chen, X., Jiang, J., Xie, L. and Zhang, D., 2009. Development of a class-specific monoclonal antibody-based ELISA for aflatoxins in peanut. Food Chemistry 115: 313-317.
'Development of a class-specific monoclonal antibody-based ELISA for aflatoxins in peanut ' () 115 Food Chemistry : 313 -317.
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Li, W. and Dai, S.Y., 2014. Using commercial immunoassay kits for mycotoxins: ‘joys and sorrows’? World Mycotoxin Journal 7: 417-430.
'Using commercial immunoassay kits for mycotoxins: ‘joys and sorrows’? ' () 7 World Mycotoxin Journal : 417 -430.
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Liang, J.M., Xayamongkhon, H., Broz, K., Dong, Y., McCormick, S.P., Abramova, S., Ward, T.J., Ma, Z.H. and Kistler, H.C., 2014. Temporal dynamics and population genetic structure ofFusarium graminearum in the upper midwestern United States. Fungal Genetics and Biology 73: 83-92.
'Temporal dynamics and population genetic structure ofFusarium graminearum in the upper midwestern United States ' () 73 Fungal Genetics and Biology : 83 -92.
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Lofgren, L., Riddle, J., Dong, Y., Kuhnem, P.R., Cummings, J.A., Del Ponte, E.M., Bergstrom, G.C. and Kistler, H.C., 2018. A high proportion of NX-2 genotype strains are found amongFusarium graminearum isolates from northeastern New York State. European Journal of Plant Pathology 150: 791-796.
'A high proportion of NX-2 genotype strains are found amongFusarium graminearum isolates from northeastern New York State ' () 150 European Journal of Plant Pathology : 791 -796.
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Maragos, C.M. and McCormick, S.P., 2000. Monoclonal antibodies for the mycotoxins deoxynivalenol and 3-acetyl-deoxynivalenol. Food and Agricultural Immunology 12: 181-192.
'Monoclonal antibodies for the mycotoxins deoxynivalenol and 3-acetyl-deoxynivalenol ' () 12 Food and Agricultural Immunology : 181 -192.
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Maragos, C.M., Li, L. and Chen, D., 2012. Production and characterization of a single chain variable fragment (scFv) against the mycotoxin deoxynivalenol. Food and Agricultural Immunology 23: 51-67.
'Production and characterization of a single chain variable fragment (scFv) against the mycotoxin deoxynivalenol ' () 23 Food and Agricultural Immunology : 51 -67.
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Michlmayr, H., Varga, E., Malachova, A., Nguyen, N.T., Lorenz, C., Haltrich, D., Berthiller, F. and Adam, G., 2015. A versatile family 3 glycoside hydrolase fromBifidobacterium adolescentis hydrolyzes β-glucosides of theFusarium mycotoxins deoxynivalenol, nivalenol, and HT-2 toxin in cereal matrices. Applied and Environmental Microbiology 81: 4885-4893.
'A versatile family 3 glycoside hydrolase fromBifidobacterium adolescentis hydrolyzes β-glucosides of theFusarium mycotoxins deoxynivalenol, nivalenol, and HT-2 toxin in cereal matrices ' () 81 Applied and Environmental Microbiology : 4885 -4893.
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Papadopoulou-Bouraoui, A., Vrabcheva, T., Valzacchi, S., Stroka, J. and Anklam., E., 2004. Screening survey of deoxynivalenol in beer from the European market by an enzyme-linked immunosorbent assay. Food Additives and Contaminants 21: 607-617.
'Screening survey of deoxynivalenol in beer from the European market by an enzyme-linked immunosorbent assay ' () 21 Food Additives and Contaminants : 607 -617.
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Ruprich, J. and Ostrý, V., 2008. Immunochemical methods in health risk assessment: cross reactivity of antibodies against mycotoxin deoxynivalenol with deoxynivalenol-3-glucoside. Central European Journal of Public Health 16: 34-37.
'Immunochemical methods in health risk assessment: cross reactivity of antibodies against mycotoxin deoxynivalenol with deoxynivalenol-3-glucoside ' () 16 Central European Journal of Public Health : 34 -37.
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'Global occurrence of mycotoxins in the food and feed chain: facts and figures ' () 6 World Mycotoxin Journal : 213 -222.
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Tangni, E.K., Motte, J.-C., Callebaut, A. and Pussemier, L., 2010. Cross-reactivity of antibodies in some commercial deoxynivalenol test kits against some fusariotoxins. Journal of Agricultural and Food Chemistry 58: 12625-12633.
'Cross-reactivity of antibodies in some commercial deoxynivalenol test kits against some fusariotoxins ' () 58 Journal of Agricultural and Food Chemistry : 12625 -12633.
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Varga, E., Wiesenberger, G., Hametner, C., Ward, T.J., Dong, Y., Schöfbeck, D., McCormick, S., Broz, K., Stückler, R., Schuhmacher, R., Krska, R., Kistler, H.C., Berthiller, F. and Adam, G., 2015. New tricks of an old enemy: isolates ofFusarium graminearum produce a type A trichothecene mycotoxin. Environmental Microbiology 17: 2588-2600.
'New tricks of an old enemy: isolates ofFusarium graminearum produce a type A trichothecene mycotoxin ' () 17 Environmental Microbiology : 2588 -2600.
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Ward, T.J., Clear, R.M., Rooney, A.P., O’Donnel, K., Gaba, D., Patrick, S., Starkey, D.E., Gilbert, J., Geiser, D.M. and Nowicki, T.W., 2008. An adaptive evolutionary shift inFusarium head blight pathogen populations is driving the rapid spread of more toxigenicFusarium graminearum in North America. Fungal Genetics and Biology 45: 473-484.
'An adaptive evolutionary shift inFusarium head blight pathogen populations is driving the rapid spread of more toxigenicFusarium graminearum in North America ' () 45 Fungal Genetics and Biology : 473 -484.
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Zachariasova, M., Cuhra, P. and Hajslova, J., 2014. Cross-reactivity of rapid immunochemical methods for mycotoxins detection towards metabolites and masked mycotoxins: the current state of knowledge. World Mycotoxin Journal 7: 449-464.
'Cross-reactivity of rapid immunochemical methods for mycotoxins detection towards metabolites and masked mycotoxins: the current state of knowledge ' () 7 World Mycotoxin Journal : 449 -464.
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Zachariasova, M., Hajslova, J., Kostelanska, M., Poustka, J., Krplova, A., Cuhra, P. and Hochel, I., 2008. Deoxynivalenol and its conjugates in beer: a critical assessment of data obtained by enzyme-linked immunosorbent assay and liquid chromatography coupled to tandem mass spectrometry. Analytica Chimica Acta 625: 77-86.
'Deoxynivalenol and its conjugates in beer: a critical assessment of data obtained by enzyme-linked immunosorbent assay and liquid chromatography coupled to tandem mass spectrometry ' () 625 Analytica Chimica Acta : 77 -86.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 0 | 0 | 0 |
| Gesamttextansichten | 377 | 122 | 19 |
| PDF-Downloads | 187 | 82 | 6 |
Cross-reactivity of commercial and non-commercial deoxynivalenol-antibodies to emerging trichothecenes and common deoxynivalenol-derivatives
in World Mycotoxin JournalSearch for other papers by N.T. Nguyen in
Current site
Google Scholar
PubMed
Search for other papers by E. Varga in
Current site
Google Scholar
PubMed
Search for other papers by C. Maragos in
Current site
Google Scholar
PubMed
Search for other papers by S. Baumgartner in
Current site
Google Scholar
PubMed
Search for other papers by G. Adam in
Current site
Google Scholar
PubMed
Search for other papers by F. Berthiller in
Current site
Google Scholar
PubMed
Immunoassay based techniques are an important and fast option for the detection and quantification of mycotoxins. They are frequently used as on-site screening tools in grain elevators, storage and production facilities. However, accurate quantification may be hampered by the co-recognition of structurally related metabolites by the used antibodies. Therefore, it is crucial to assess their cross-reactivity to avoid misinterpretation of the results. Several immunoassays for the determination of deoxynivalenol (DON) are commercially available. Recently, novel trichothecene mycotoxins with structures similar to DON, the NX-toxins (NX-2, NX-3 and NX-4), were discovered, which can potentially co-occur with DON in cereals. So far, no data about the cross-reactivity of those toxins with DON-antibodies are available. The aim of this study was to assess the cross-reactivities of NX-toxins and some other DON-related metabolites with DON-antibodies in buffer solutions. Six commercially available enzyme-linked immunosorbent assays (ELISAs) and two previously developed DON-antibodies (Mab#1 and Mab#22) were tested. Cross-reactivity with NX-metabolites was not observed for any of the ELISA-kits nor Mab#22, whereas Mab#1 reacted moderately against NX-3 and NX-4 (cross-reactivity based on a molar basis of 14 and 30%, respectively). Modifications at position C-3 (3-acetyl-DON and DON-3-glucoside) led to moderate or high cross-reactivity with Mab#22 and the commercial ELISA-kits, whereas these compounds were not recognised by Mab#1. Similar to NX-metabolites, 15-acetyl-DON interacted only weakly with Mab#22 and the commercial ELISA-kits, but strongly with Mab#1. The results demonstrate the importance of proper antibody characterisation. If NX-metabolites prove to be widely distributed and reach significant levels, the development of specific antibodies targeting these novel metabolites might become necessary.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 0 | 0 | 0 |
| Gesamttextansichten | 377 | 122 | 19 |
| PDF-Downloads | 187 | 82 | 6 |
