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Fumonisins are among the most prevalent mycotoxins in feedstuffs. They disrupt the sphingolipid metabolism, thereby inducing a plethora of toxic effects in livestock. Supplementation with mycotoxin-degrading enzymes is a promising strategy for the detoxification of feedstuffs in the animals’ gastrointestinal tract. Here, we evaluated the suitability of the fumonisin esterase FumD as a feed additive (FUMzyme®) for the prevention of fumonisin toxicity in pigs by using a combination of different fumonisin biomarkers (sphinganine to sphingosine (Sa/So) ratio in serum and organs, concentrations of fumonisin B1 and hydrolysed derivatives in urine and faeces). In a pre-trial, we exposed pigs to 30 mg/kg fumonisins in feed and found the minimum effective dose of FUMzyme to be 15 U/kg. In a second trial we investigated the long-term efficacy of this minimum effective FUMzyme dose to counteract toxic effects elicited by 6 weeks of exposure to 2.5 mg/kg fumonisins in a diet containing naturally contaminated maize. Supplementation of feed with the minimum effective FUMzyme dose prevented an increase in the Sa/So ratio in serum and kidneys of fumonisin exposed pigs. The Sa/So ratio in serum proved to be the most reliable biomarker. The fumonisin pattern in faeces was less suitable as biomarker for assessing the efficacy of FUMzyme due to natural gastrointestinal hydrolysis of fumonisins. Analysis of urine samples provided additional information about gastrointestinal fumonisin hydrolysis before fumonisin absorption, but was analytically challenging because of low urinary fumonisin concentrations.
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'The food contaminant fumonisin B1 reduces the maturation of porcine CD11R1+ intestinal antigen presenting cells and antigen-specific immune responses, leading to a prolonged intestinal ETEC infection ' () 40 Veterinary Research : 40.
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'In vitro microbial metabolism of fumonisin B1 ' () 24 Food Additives and Contaminants : 416 -420.
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Grenier, B., Bracarense, A.P., Schwartz, H.E., Trumel, C., Cossalter, A.M., Schatzmayr, G., Kolf-Clauw, M., Moll, W.D. and Oswald, I.P., 2012. The low intestinal and hepatic toxicity of hydrolyzed fumonisin B1 correlates with its inability to alter the metabolism of sphingolipids. Biochemical Pharmacology 83: 1465-1473.
'The low intestinal and hepatic toxicity of hydrolyzed fumonisin B1 correlates with its inability to alter the metabolism of sphingolipids ' () 83 Biochemical Pharmacology : 1465 -1473.
Grenier, B., Bracarense, A.P., Schwartz, H.E., Lucioli, J., Cossalter, A.M., Moll, W.D., Schatzmayr, G. and Oswald, I.P., 2013. Biotransformation approaches to alleviate the effects induced byfusarium mycotoxins in swine. Journal of Agricultural and Food Chemistry 61: 6711-6719.
'Biotransformation approaches to alleviate the effects induced byfusarium mycotoxins in swine ' () 61 Journal of Agricultural and Food Chemistry : 6711 -6719.
Grenier, B., Schwartz-Zimmermann, H.E., Caha, S., Moll, W.D., Schatzmayr, G. and Applegate, T.J., 2015. Dose-dependent effects on sphingoid bases and cytokines in chickens fed diets prepared with fusarium verticillioides culture material containing fumonisins. Toxins 7: 1253-1272.
'Dose-dependent effects on sphingoid bases and cytokines in chickens fed diets prepared with fusarium verticillioides culture material containing fumonisins ' () 7 Toxins : 1253 -1272.
Gumprecht, L.A., Beasley, V.R., Weigel, R.M., Parker, H.M., Tumbleson, M.E., Bacon, C.W., Meredith, F.I. and Haschek, W.M., 1998. Development of fumonisin-induced hepatotoxicity and pulmonary edema in orally dosed swine: morphological and biochemical alterations. Toxicologic Pathology 26: 777-788.
'Development of fumonisin-induced hepatotoxicity and pulmonary edema in orally dosed swine: morphological and biochemical alterations ' () 26 Toxicologic Pathology : 777 -788.
Hahn, I., Nagl, V., Schwartz-Zimmermann, H.E., Varga, E., Schwarz, C., Slavik, V., Reisinger, N., Malachova, A., Cirlini, M., Generotti, S., Dall’Asta, C., Krska, R., Moll, W.D. and Berthiller, F., 2015. Effects of orally administered fumonisin B1 (FB1), partially hydrolysed FB1, hydrolysed FB1 and N-(1-deoxy-D-fructos-1-yl) FB1 on the sphingolipid metabolism in rats. Food and Chemical Toxicology 76: 11-18.
'Effects of orally administered fumonisin B1 (FB1), partially hydrolysed FB1, hydrolysed FB1 and N-(1-deoxy-D-fructos-1-yl) FB1 on the sphingolipid metabolism in rats ' () 76 Food and Chemical Toxicology : 11 -18.
Hartinger, D. and Moll, W.D., 2011. Fumonisin elimination and prospects for detoxification by enzymatic transformation. World Mycotoxin Journal 4: 271-283.
'Fumonisin elimination and prospects for detoxification by enzymatic transformation ' () 4 World Mycotoxin Journal : 271 -283.
Haschek, W.M., Gumprecht, L.A., Smith, G., Tumbleson, M.E. and Constable, P.D., 2001. Fumonisin toxicosis in swine: an overview of porcine pulmonary edema and current perspectives. Environmental Health Perspectives 109, Suppl. 2: 251-257.
'Fumonisin toxicosis in swine: an overview of porcine pulmonary edema and current perspectives ' () 109 Environmental Health Perspectives : 251 -257.
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'Degradation of fumonisin B1 by the consecutive action of two bacterial enzymes ' () 145 Journal of Biotechnology : 120 -129.
Howard, P.C., Couch, L.H., Patton, R.E., Eppley, R.M., Doerge, D.R., Churchwell, M.I., Marques, M.M. and Okerberg, C.V., 2002. Comparison of the toxicity of several fumonisin derivatives in a 28-day feeding study with female B6C3F1 mice. Toxicology and Applied Pharmacology 185: 153-165.
'Comparison of the toxicity of several fumonisin derivatives in a 28-day feeding study with female B6C3F1 mice ' () 185 Toxicology and Applied Pharmacology : 153 -165.
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'Gastrointestinal degradation of fumonisin B1 by carboxylesterase FumD prevents fumonisin induced alteration of sphingolipid metabolism in turkey and swine ' () 8 Toxins : 84.
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Mitchell, N.J., Xue, K.S., Marroquin-Cardona, A., Brown, K.A., Elmore, S.E., Tang, L., Romoser, A., Gelderblom, W.C.A., Wang, J.-S. and Phillips, T.D., 2014. Calcium montmorillonite clay reduces AFB1 and FB1 biomarkers in rats exposed to single and co-exposures of aflatoxin and fumonisin. Journal of Applied Toxicology 34: 795-804.
'Calcium montmorillonite clay reduces AFB1 and FB1 biomarkers in rats exposed to single and co-exposures of aflatoxin and fumonisin ' () 34 Journal of Applied Toxicology : 795 -804.
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'Global occurrence of mycotoxins in the food and feed chain: facts and figures ' () 6 World Mycotoxin Journal : 213 -222.
Taranu, I., Marin, D.E., Bouhet, S., Pascale, F., Bailly, J.D., Miller, J.D., Pinton, P. and Oswald, I.P., 2005. Mycotoxin fumonisin B1 alters the cytokine profile and decreases the vaccinal antibody titer in pigs. Toxicological Sciences 84: 301-307.
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'Reproductive and sphingolipid metabolic effects of fumonisin B1 and its alkaline hydrolysis product in LM/Bc mice: hydrolyzed fumonisin B1 did not cause neural tube defects ' () 112 Toxicological Sciences : 459 -467.
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Fumonisins are among the most prevalent mycotoxins in feedstuffs. They disrupt the sphingolipid metabolism, thereby inducing a plethora of toxic effects in livestock. Supplementation with mycotoxin-degrading enzymes is a promising strategy for the detoxification of feedstuffs in the animals’ gastrointestinal tract. Here, we evaluated the suitability of the fumonisin esterase FumD as a feed additive (FUMzyme®) for the prevention of fumonisin toxicity in pigs by using a combination of different fumonisin biomarkers (sphinganine to sphingosine (Sa/So) ratio in serum and organs, concentrations of fumonisin B1 and hydrolysed derivatives in urine and faeces). In a pre-trial, we exposed pigs to 30 mg/kg fumonisins in feed and found the minimum effective dose of FUMzyme to be 15 U/kg. In a second trial we investigated the long-term efficacy of this minimum effective FUMzyme dose to counteract toxic effects elicited by 6 weeks of exposure to 2.5 mg/kg fumonisins in a diet containing naturally contaminated maize. Supplementation of feed with the minimum effective FUMzyme dose prevented an increase in the Sa/So ratio in serum and kidneys of fumonisin exposed pigs. The Sa/So ratio in serum proved to be the most reliable biomarker. The fumonisin pattern in faeces was less suitable as biomarker for assessing the efficacy of FUMzyme due to natural gastrointestinal hydrolysis of fumonisins. Analysis of urine samples provided additional information about gastrointestinal fumonisin hydrolysis before fumonisin absorption, but was analytically challenging because of low urinary fumonisin concentrations.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 0 | 0 | 0 |
| Gesamttextansichten | 370 | 104 | 17 |
| PDF-Downloads | 232 | 96 | 3 |