Save

Application of biomarker methods to investigate FUMzyme mediated gastrointestinal hydrolysis of fumonisins in pigs

in World Mycotoxin Journal
Autor:innen:
H.E. Schwartz-Zimmermann Christian Doppler Laboratory for Mycotoxin Metabolism, Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna, 3430 Tulln, Austria.

Search for other papers by H.E. Schwartz-Zimmermann in
Current site
Google Scholar
PubMed
Close
,
D. Hartinger BIOMIN Research Center, Technopark 1, 3430 Tulln, Austria.

Search for other papers by D. Hartinger in
Current site
Google Scholar
PubMed
Close
,
B. Doupovec BIOMIN Research Center, Technopark 1, 3430 Tulln, Austria.

Search for other papers by B. Doupovec in
Current site
Google Scholar
PubMed
Close
,
C. Gruber-Dorninger BIOMIN Research Center, Technopark 1, 3430 Tulln, Austria.

Search for other papers by C. Gruber-Dorninger in
Current site
Google Scholar
PubMed
Close
,
M. Aleschko BIOMIN Research Center, Technopark 1, 3430 Tulln, Austria.

Search for other papers by M. Aleschko in
Current site
Google Scholar
PubMed
Close
,
S. Schaumberger BIOMIN Research Center, Technopark 1, 3430 Tulln, Austria.

Search for other papers by S. Schaumberger in
Current site
Google Scholar
PubMed
Close
,
V. Nagl Christian Doppler Laboratory for Mycotoxin Metabolism, Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna, 3430 Tulln, Austria.
BIOMIN Research Center, Technopark 1, 3430 Tulln, Austria.

Search for other papers by V. Nagl in
Current site
Google Scholar
PubMed
Close
,
I. Hahn Christian Doppler Laboratory for Mycotoxin Metabolism, Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna, 3430 Tulln, Austria.

Search for other papers by I. Hahn in
Current site
Google Scholar
PubMed
Close
,
F. Berthiller Christian Doppler Laboratory for Mycotoxin Metabolism, Center for Analytical Chemistry, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna, 3430 Tulln, Austria.

Search for other papers by F. Berthiller in
Current site
Google Scholar
PubMed
Close
,
D. Schatzmayr BIOMIN Research Center, Technopark 1, 3430 Tulln, Austria.

Search for other papers by D. Schatzmayr in
Current site
Google Scholar
PubMed
Close
, und
W.D. Moll BIOMIN Research Center, Technopark 1, 3430 Tulln, Austria.

Search for other papers by W.D. Moll in
Current site
Google Scholar
PubMed
Close

Fumonisins are among the most prevalent mycotoxins in feedstuffs. They disrupt the sphingolipid metabolism, thereby inducing a plethora of toxic effects in livestock. Supplementation with mycotoxin-degrading enzymes is a promising strategy for the detoxification of feedstuffs in the animals’ gastrointestinal tract. Here, we evaluated the suitability of the fumonisin esterase FumD as a feed additive (FUMzyme®) for the prevention of fumonisin toxicity in pigs by using a combination of different fumonisin biomarkers (sphinganine to sphingosine (Sa/So) ratio in serum and organs, concentrations of fumonisin B1 and hydrolysed derivatives in urine and faeces). In a pre-trial, we exposed pigs to 30 mg/kg fumonisins in feed and found the minimum effective dose of FUMzyme to be 15 U/kg. In a second trial we investigated the long-term efficacy of this minimum effective FUMzyme dose to counteract toxic effects elicited by 6 weeks of exposure to 2.5 mg/kg fumonisins in a diet containing naturally contaminated maize. Supplementation of feed with the minimum effective FUMzyme dose prevented an increase in the Sa/So ratio in serum and kidneys of fumonisin exposed pigs. The Sa/So ratio in serum proved to be the most reliable biomarker. The fumonisin pattern in faeces was less suitable as biomarker for assessing the efficacy of FUMzyme due to natural gastrointestinal hydrolysis of fumonisins. Analysis of urine samples provided additional information about gastrointestinal fumonisin hydrolysis before fumonisin absorption, but was analytically challenging because of low urinary fumonisin concentrations.

Kennzahlen

Insgesamt Letzte 365 Tage In den letzten 30 Tagen
Aufrufe von Kurzbeschreibungen 0 0 0
Gesamttextansichten 370 104 17
PDF-Downloads 232 96 3