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In vitro assessment of protein digestibility and mineral solubility of black soldier fly larvae meals for monogastric animals
in Journal of Insects as Food and Feed
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M.S. Silva
https://orcid.org/0000-0001-8691-4054
M.S. Silva
Institute of Marine Research, P.O. Box 1870 Nordnes, 5817 Bergen, Norway.
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R. Matos
R. Matos
Institute of Marine Research, P.O. Box 1870 Nordnes, 5817 Bergen, Norway.
Department of Biological Sciences, University of Bergen, P.O. Box 7803, 5020 Bergen, Norway.
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Department of Biological Sciences, University of Bergen, P.O. Box 7803, 5020 Bergen, Norway.
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P. Araujo
P. Araujo
Institute of Marine Research, P.O. Box 1870 Nordnes, 5817 Bergen, Norway.
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E.J. Lock
E.J. Lock
Institute of Marine Research, P.O. Box 1870 Nordnes, 5817 Bergen, Norway.
Department of Biological Sciences, University of Bergen, P.O. Box 7803, 5020 Bergen, Norway.
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Department of Biological Sciences, University of Bergen, P.O. Box 7803, 5020 Bergen, Norway.
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R. Gopika
R. Gopika
Institute of Marine Research, P.O. Box 1870 Nordnes, 5817 Bergen, Norway.
Department of Biological Sciences, University of Bergen, P.O. Box 7803, 5020 Bergen, Norway.
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Department of Biological Sciences, University of Bergen, P.O. Box 7803, 5020 Bergen, Norway.
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P.A.J. Prabhu
P.A.J. Prabhu
Institute of Marine Research, P.O. Box 1870 Nordnes, 5817 Bergen, Norway.
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I. Belghit
I. Belghit
Institute of Marine Research, P.O. Box 1870 Nordnes, 5817 Bergen, Norway.
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Animal farming is a fast-growing sector which demands a large supply of feed materials and the search for novel feed ingredients with a smaller impact on the global environment has been intensified. However, before the inclusion of these feed ingredients in animal feed, a preliminary assessment of the quality and performance is needed. This evaluation is usually done byin vivo feeding trials, butin vitro digestion methods have been successfully used for gathering information regarding the nutritional value of the different feed components. Therefore, in this work, anin vitro digestion method was developed using black soldier fly (BSF) larvae meals. The method included seven experimental variables, namely: pH in stomach, pH in intestine, reaction time in stomach, reaction time in intestine, enzyme concentration in stomach, enzyme concentration in intestine and temperature. The experimental variables were screened by means of a fractional factorial design (27-2) that allowed to select the concentration of enzyme in the intestine and temperature (at 7 U and 37 °C, respectively) as the most influential factors on amino acid and mineral solubility in BSF larvae meal. Performing thein vitro digestibility at 37 °C with pH of the stomach and intestine at 2 and 9, reaction time in the stomach and intestine at 1 hour and 4 hours and concentration of enzyme in the stomach and intestine at 1 U and 7 U resulted in the highest overall nutrient solubility. This optimisedin vitro digestion methodology was used to assess the nutritional stability of three batches of BSF meals delivered by the same producer. Thein vitro digestion method was able to reveal differences between the three batches of BSF meals.
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In vitro assessment of protein digestibility and mineral solubility of black soldier fly larvae meals for monogastric animals
in Journal of Insects as Food and Feed
Autor:innen:
,
,
,
,
,
, und
M.S. Silva
https://orcid.org/0000-0001-8691-4054
M.S. Silva
Institute of Marine Research, P.O. Box 1870 Nordnes, 5817 Bergen, Norway.
Search for other papers by M.S. Silva in
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R. Matos
R. Matos
Institute of Marine Research, P.O. Box 1870 Nordnes, 5817 Bergen, Norway.
Department of Biological Sciences, University of Bergen, P.O. Box 7803, 5020 Bergen, Norway.
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Department of Biological Sciences, University of Bergen, P.O. Box 7803, 5020 Bergen, Norway.
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P. Araujo
P. Araujo
Institute of Marine Research, P.O. Box 1870 Nordnes, 5817 Bergen, Norway.
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E.J. Lock
E.J. Lock
Institute of Marine Research, P.O. Box 1870 Nordnes, 5817 Bergen, Norway.
Department of Biological Sciences, University of Bergen, P.O. Box 7803, 5020 Bergen, Norway.
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Department of Biological Sciences, University of Bergen, P.O. Box 7803, 5020 Bergen, Norway.
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R. Gopika
R. Gopika
Institute of Marine Research, P.O. Box 1870 Nordnes, 5817 Bergen, Norway.
Department of Biological Sciences, University of Bergen, P.O. Box 7803, 5020 Bergen, Norway.
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Department of Biological Sciences, University of Bergen, P.O. Box 7803, 5020 Bergen, Norway.
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P.A.J. Prabhu
P.A.J. Prabhu
Institute of Marine Research, P.O. Box 1870 Nordnes, 5817 Bergen, Norway.
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I. Belghit
I. Belghit
Institute of Marine Research, P.O. Box 1870 Nordnes, 5817 Bergen, Norway.
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Animal farming is a fast-growing sector which demands a large supply of feed materials and the search for novel feed ingredients with a smaller impact on the global environment has been intensified. However, before the inclusion of these feed ingredients in animal feed, a preliminary assessment of the quality and performance is needed. This evaluation is usually done byin vivo feeding trials, butin vitro digestion methods have been successfully used for gathering information regarding the nutritional value of the different feed components. Therefore, in this work, anin vitro digestion method was developed using black soldier fly (BSF) larvae meals. The method included seven experimental variables, namely: pH in stomach, pH in intestine, reaction time in stomach, reaction time in intestine, enzyme concentration in stomach, enzyme concentration in intestine and temperature. The experimental variables were screened by means of a fractional factorial design (27-2) that allowed to select the concentration of enzyme in the intestine and temperature (at 7 U and 37 °C, respectively) as the most influential factors on amino acid and mineral solubility in BSF larvae meal. Performing thein vitro digestibility at 37 °C with pH of the stomach and intestine at 2 and 9, reaction time in the stomach and intestine at 1 hour and 4 hours and concentration of enzyme in the stomach and intestine at 1 U and 7 U resulted in the highest overall nutrient solubility. This optimisedin vitro digestion methodology was used to assess the nutritional stability of three batches of BSF meals delivered by the same producer. Thein vitro digestion method was able to reveal differences between the three batches of BSF meals.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
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