Efficacy of probiotic Bacillus licheniformis DSM 28710 on performance and the mitigation of Clostridium perfringens-induced necrotic enteritis in broiler chickens
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The aim of this study was to evaluate the impact of a probiotic Bacillus licheniformis strain (DSM 28710; B-Act®) on growth performance and its capacity to mitigate necrotic enteritis (NE; induced via a Clostridium perfringens challenge) in poultry. A broiler trial was conducted, examining three treatments for 42 days under an induced NE challenge; a negative control (basal diet only); an antibiotic treated group (oxytetracycline hydrochloride (OXT), therapeutic dose of 105 mg OXT/litre in drinking water, for three days after C. perfringens challenge); and a B-Act group (500 g B-Act/tonne of feed, equalling 1.6×1012 colony forming units B. licheniformis DSM 28710/tonne of feed, supplemented from start until finish). Despite the induced NE challenge, weight gains of the B-Act and OXT groups were similar to each other but significantly higher compared to the control at the end of the study (P<0.05). Weight gain of the B-Act group was already significantly higher compared to the control on day 21 (P<0.05), indicating a potential benefit of the probiotic even before clinical establishment of NE. Feed conversion ratio (FCR) values followed a similar pattern throughout the study, with a significantly lower overall FCR for the B-Act and OXT groups compared to the control (P<0.05; d0-42). Birds fed B-Act had significantly (P<0.05) lower NE lesions compared to the control and OXT group on day 21, although OXT was not supplemented to the animals at this stage yet. Both B-Act and OXT groups had significantly (P<0.05) lower NE scores than the control on day 28, demonstrating the effectiveness of the antibiotic treatment and the mitigating effect of B-Act on the effects of a Clostridium perfringens induced NE challenge.
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Antonissen, G., Eeckhaut, V., Van Driessche, K., Onrust, L., Haesebrouck, F., Ducatelle, R., Moore R.J. and Van Immerseel, F., 2016. Microbial shifts associated with necrotic enteritis. Avian Pathology 45(3): 308-312.
'Microbial shifts associated with necrotic enteritis ' () 45 Avian Pathology : 308 -312.
-
Barbosa, T.M., Serra, C.R., La Ragione, R.M., Woodward, M.J. and Henriques, A.O., 2005. Screening for Bacillus isolates in the broiler gastrointestinal tract. Applied and Environmental Microbiology 71: 968-978.
'Screening for Bacillus isolates in the broiler gastrointestinal tract ' () 71 Applied and Environmental Microbiology : 968 -978.
-
Bernardeau, M.J., Lehtinen, M.J., Forssten, S.D. and Nurminen, P., 2017. Importance of the gastrointestinal life cycle of Bacillus for probiotic functionality. Journal of Food Science and Technology 54(8): 2570-2584.
'Importance of the gastrointestinal life cycle of Bacillus for probiotic functionality ' () 54 Journal of Food Science and Technology : 2570 -2584.
-
Caly, D.L., D’Inca, R., Auclair, E. and Drider, D., 2015. Alternatives to antibiotics to prevent necrotic enteritis in broiler chickens: a microbiologist’s perspective. Frontiers in Microbiology 6: 1336.
'Alternatives to antibiotics to prevent necrotic enteritis in broiler chickens: a microbiologist’s perspective ' () 6 Frontiers in Microbiology : 1336.
-
Casewell, M., Friis, C., Marco, E., McMullin, P. and Phillips, I., 2003. The European ban on growth-promoting antibiotics and emerging consequences for human and animal health. Journal of Antimicrobial Chemotherapy 52: 159-161.
'The European ban on growth-promoting antibiotics and emerging consequences for human and animal health ' () 52 Journal of Antimicrobial Chemotherapy : 159 -161.
-
Cobb, 2016. Broiler performance & nutrition supplement. Cobb-Vantress, Siloam Springs, AR, USA, 14 pp. Available at: https://tinyurl.com/y4nn8vvg.
-
Deng, W., Dong, X.F., Tong, J.M. and Zhang, Q., 2012. The probiotic Bacillus licheniformis ameliorates heat stress-induced impairment of egg production, gut morphology and intestinal mucosal immunity in laying hens. Poultry Science 91: 575-582.
'The probiotic Bacillus licheniformis ameliorates heat stress-induced impairment of egg production, gut morphology and intestinal mucosal immunity in laying hens ' () 91 Poultry Science : 575 -582.
-
Dischinger, J., Josten, M., Szekat, C., Sahl, H.G. and Bierbaum, G., 2009. Production of the novel two-peptide lantibiotic lichenicidin by Bacillus licheniformis DSM 13. PLoS ONE 4(8): e6788. https://doi.org/10.1371/journal.pone.0006788
-
Elshaghabee, F.M.F., Rokana, N., Gulhane, R.D., Sharma, C. and Panwar, H., 2017. Bacillus as potential probiotics: status, concerns, and future perspectives. Frontiers in Microbiology 8: 1490. https://doi.org/10.3389/fmicb.2017.01490
-
European Food Safety Authority (EFSA), 2016. Safety and efficacy of B-Act® (Bacillus licheniformis DSM 28710) for chickens for fattening and chickens reared for laying. EFSA Journal 14(11): 4615.
-
European Food Safety Authority (EFSA), 2019. Safety and efficacy of B-Act® (Bacillus licheniformis DSM 28710) as a feed additive for turkeys for fattening, turkeys reared for breeding and minor poultry species for fattening or raised for laying. EFSA Journal 17(1): 5536.
-
Food and Agriculture Organization (FAO), 2016. Probiotics in animal nutrition: production, impact and regulation. Food and Agriculture Organization of the United Nations Technical Report. Food and Agriculture Organization, Rome, Italy, 108 pp. Available at: https://tinyurl.com/y5k652hh.
-
Food and Agriculture Organization/World Health Organization (FAO/WHO), 2002. Guidelines for the evaluation of probiotics in food. Food and Agriculture Organization of the United Nations and World Health Organization Working Group Report. Food and Agriculture Organization, Rome, Italy, 11 pp. Available at: https://tinyurl.com/y4guddsx.
-
Hofacre, C.L., Beacorn, T., Collett, S. and Mathis, G., 2003. Using competitive exclusion mannan-oligosaccharide and other intestinal products to control necrotic enteritis. Journal of Applied Poultry Research 12: 60-64.
'Using competitive exclusion mannan-oligosaccharide and other intestinal products to control necrotic enteritis ' () 12 Journal of Applied Poultry Research : 60 -64.
-
Hofacre, C.L., Froyman, R., Gautrias, B., Georgie, B., Goodwin, M.A. and Brown, J., 1998. Use of Aviguard and other intestinal bioproducts in experimental Clostridium perfringens-associated necrotizing enteritis in broiler chickens. Avian Diseases 42: 579-584.
'Use of Aviguard and other intestinal bioproducts in experimental Clostridium perfringens-associated necrotizing enteritis in broiler chickens ' () 42 Avian Diseases : 579 -584.
-
Kaldhusdal, M., Benestad, S.L. and Lovland A., 2016. Epidemiologic aspects of necrotic enteritis in broiler chickens – disease occurrence and production performance. Avian Pathology 45(3): 271-274.
'Epidemiologic aspects of necrotic enteritis in broiler chickens – disease occurrence and production performance ' () 45 Avian Pathology : 271 -274.
-
Knap, I., Lund, B., Kehlet, A.B., Hofacre, C. and Mathis, G., 2010. Bacillus licheniformis prevents necrotic enteritis in broiler chickens. Avian Disease 54(2): 931-935.
'Bacillus licheniformis prevents necrotic enteritis in broiler chickens ' () 54 Avian Disease : 931 -935.
-
Lei, K., Li, L., Yu, D.Y., Rajput, L.R. and Li, W.F., 2013. Influence of dietary inclusion of Bacillus licheniformis on laying performance, egg quality, antioxidant enzymes activities and intestinal barrier function of laying hens. Poultry Science 92: 2389-2395.
'Influence of dietary inclusion of Bacillus licheniformis on laying performance, egg quality, antioxidant enzymes activities and intestinal barrier function of laying hens ' () 92 Poultry Science : 2389 -2395.
-
Lin, E.R., Cheng, Y.H., Hsiao, F.S.H., Proskura, W.S., Dybus, A. and Yu, Y.H., 2019. Optimization of solid-state fermentation conditions of Bacillus licheniformis and its effects on Clostridium perfringens-induced necrotic enteritis in broilers. Revista Brasileira de Zootecnia 48: 0298. https://doi.org/10.1590/rbz4820170298
-
Lin, Y., Xu, S., Zeng, D., Ni, X., Zhou, M., Zeng, Y., Wang, H., Zhou, Y., Zhu, H., Pan, K. and Li, G., 2017. Disruption in the cecal microbiotia of chickens challenged with Clostridium perfringens and other factors was alleviated by Bacillus licheniformis supplementation. PLoS ONE 12(8): e0182426. https://doi.org/10.1371/journal.pone.0182426
-
Mingmongkolchai, S. and Panbangred, W., 2018. Bacillus probiotics: an alternative to antibiotics for livestock production. Journal of Applied Microbiology 124: 1334-1346.
'Bacillus probiotics: an alternative to antibiotics for livestock production ' () 124 Journal of Applied Microbiology : 1334 -1346.
-
M’Sadeq, S.A., Wu, S., Swick, R.A. and Choct, M., 2015. Towards the control of necrotic enteritis in broiler chickens with in-feed antibiotics phasing-out worldwide. Animal Nutrition 1(1): 1-11.
'Towards the control of necrotic enteritis in broiler chickens with in-feed antibiotics phasing-out worldwide ' () 1 Animal Nutrition : 1 -11.
-
Simon, O., Jadamus, A. and Vahjen, W., 2001. Probiotic feed effects – effectiveness and expected modes of action. Journal of Animal and Feed Sciences 10(1): 51-67.
'Probiotic feed effects – effectiveness and expected modes of action ' () 10 Journal of Animal and Feed Sciences : 51 -67.
-
Timbermont, L., Haesebrouck, F., Ducatelle, R. and Van Immerseel, F., 2011. Necrotic enteritis in broilers: an updated review on the pathogenesis. Avian Pathology 40(4): 341-347.
'Necrotic enteritis in broilers: an updated review on the pathogenesis ' () 40 Avian Pathology : 341 -347.
-
Van der Sluis W., 2000. Clostridial enteritis is an often-underestimated problem. World Poultry 16(7): 42-43.
'Clostridial enteritis is an often-underestimated problem ' () 16 World Poultry : 42 -43.
-
Wade, B. and Keyburn, A., 2015. The true cost of necrotic enteritis. World Poultry 31(7): 16-17.
'The true cost of necrotic enteritis ' () 31 World Poultry : 16 -17.
-
Williams, R., 2005. Intercurrent coccidiosis and necrotic enteritis of chickens: rational, integrated disease management by maintenance of gut integrity. Avian Pathology 34: 159-180.
'Intercurrent coccidiosis and necrotic enteritis of chickens: rational, integrated disease management by maintenance of gut integrity ' () 34 Avian Pathology : 159 -180.
-
Xu, S., Lin, Y., Zeng, D., Zhou, M., Wang, H., Zhou, Y., Zhu, H., Pan, K., Jing, B. and Ni, X., 2018. Bacillus licheniformis normalize the ileum microbiota of chickens infected with necrotic enteritis. Scientific Reports 8: 1744.
'Bacillus licheniformis normalize the ileum microbiota of chickens infected with necrotic enteritis ' () 8 Scientific Reports : 1744.
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Zhou, M., Zeng, D., Ni, X., Tu, T., Yin, Z., Pan, K. and Jing, B., 2016. Effects of Bacillus licheniformis on the growth performance and expression of lipid metabolism-related genes in broiler chickens challenged with Clostridium perfringens-induced necrotic enteritis. Lipids in Health and Disease 15: 48. https://doi.org/10.1186/s12944-016-0219-2
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Efficacy of probiotic Bacillus licheniformis DSM 28710 on performance and the mitigation of Clostridium perfringens-induced necrotic enteritis in broiler chickens
In: Journal of Applied Animal NutritionSearch for other papers by W. Van der Veken in
Current site
Google Scholar
PubMed
Search for other papers by V. Hautekiet in
Current site
Google Scholar
PubMed
Search for other papers by E.A. Kimminau in
Current site
Google Scholar
PubMed
Search for other papers by C. Hofacre in
Current site
Google Scholar
PubMed
Search for other papers by G.F. Mathis in
Current site
Google Scholar
PubMed
The aim of this study was to evaluate the impact of a probiotic Bacillus licheniformis strain (DSM 28710; B-Act®) on growth performance and its capacity to mitigate necrotic enteritis (NE; induced via a Clostridium perfringens challenge) in poultry. A broiler trial was conducted, examining three treatments for 42 days under an induced NE challenge; a negative control (basal diet only); an antibiotic treated group (oxytetracycline hydrochloride (OXT), therapeutic dose of 105 mg OXT/litre in drinking water, for three days after C. perfringens challenge); and a B-Act group (500 g B-Act/tonne of feed, equalling 1.6×1012 colony forming units B. licheniformis DSM 28710/tonne of feed, supplemented from start until finish). Despite the induced NE challenge, weight gains of the B-Act and OXT groups were similar to each other but significantly higher compared to the control at the end of the study (P<0.05). Weight gain of the B-Act group was already significantly higher compared to the control on day 21 (P<0.05), indicating a potential benefit of the probiotic even before clinical establishment of NE. Feed conversion ratio (FCR) values followed a similar pattern throughout the study, with a significantly lower overall FCR for the B-Act and OXT groups compared to the control (P<0.05; d0-42). Birds fed B-Act had significantly (P<0.05) lower NE lesions compared to the control and OXT group on day 21, although OXT was not supplemented to the animals at this stage yet. Both B-Act and OXT groups had significantly (P<0.05) lower NE scores than the control on day 28, demonstrating the effectiveness of the antibiotic treatment and the mitigating effect of B-Act on the effects of a Clostridium perfringens induced NE challenge.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 0 | 0 | 0 |
| Full Text Views | 354 | 116 | 14 |
| PDF Views & Downloads | 344 | 108 | 13 |
