Möchten Sie über diese Zeitschrift informiert bleiben? Klicken Sie bitte auf die Buttons, um unsere Alerts zu abonnieren.
Möchten Sie über diese Zeitschrift informiert bleiben? Klicken Sie bitte auf die Buttons, um unsere Alerts zu abonnieren.
A procedure for dual simple t-cannulation in the small intestine of pigs using a single right flank laparotomy
in Journal of Applied Animal NutritionSearch for other papers by A.L. Petry in
Current site
Google Scholar
Search for other papers by S.A. Gould in
Current site
Google Scholar
Search for other papers by J.F. Patience in
Current site
Google Scholar
Singular cannulation in the small intestine of pigs is a common methodology for studying nutrient digestibility, delivering compounds into the gastrointestinal tract, or repeated tissue and digesta sampling. In that respect, it is an important tool for nutritionists and researchers. However, there is a dearth of detailed methodologies describing multiple intestinal cannulations using modern techniques. The objective of this experiment was to develop a single right flank laparotomy technique that allowed for imsertion of multiple cannulas in the small intestine, with minimal variation, and allowed for externalisation of both cannulas on the same lateral side as the laparotomy. Thirty gilts (L337 × Camborough) with an initial body weight of 30.2±0.78 kg underwent the procedure. Each gilt was equipped with a simple t-cannula in the terminal ileum, approximately 10 cm cranial to the ileocecal valve, and a second t-cannula in the jejunum 240 cm distal from where the duodenum is visually posterior to the transverse colon. The procedure used hallmarks of commonly implemented terminal ileal cannulation techniques, but modified the laparotomy location and cannula externalisation sites and used a novel approach for determining the more proximal cannula location to mitigate the need of a second laparotomy. Gilts were allowed to recover for a minimum of 7 d and were used for an average of 67 days in subsequent experimental trials. Cannula longevity was from 30 to 73 kg of body weight. Pigs were necropsied for surgical site gross examination and small intestine measurements at the end of the experiment. The jejunal cannula had a mean placement of 298.90±9.96 cm distal to the pyloric sphincter with a coefficient of variation of 3.33%. Hence, this procedure provided a single laparotomy technique for obtaining digesta from multiple locations of the small intestine, with minimal variation in proximal cannula placement.
-
Albin, D.M., Wubben, J.E. and Gabert, V.M., 1999. Approaches to collecting ileal digesta from swine. University of Illinois Extension, Champaign, IL, USA. Available at: http://livestocktrail.illinois.edu/porknet/paperDisplay.cfm?ContentID=71.
-
Beale, C.N., Reyelt, L.A., Bogins, C.A., Chan, N.Y. and Perkins, S.E., 2018. Duodenal cannulation in pigs (Sus scrofa) as a drug delivery method. Comparative Medicine 68: 48-55.
'Duodenal cannulation in pigs (Sus scrofa) as a drug delivery method ' () 68 Comparative Medicine : 48 -55.
-
Braude, R., Fulford, R.J. and Low, A.G., 1976. Studies on digestion and absorption in the intestines of growing pigs. Measurements of the flow of digesta and pH. British Journal of Nutrition 36: 497-510. https://doi.org/10.1079/BJN19760104
-
FASS, 2010. Guide for the care and use of agricultural animals in research and teaching (Ag guide): third edition. Champaign, IL, USA, 177 pp. Available at: https://tinyurl.com/y52xlfgd.
-
Furuya, S., Takahashi, S. and Omori, S., 1974. The establishment of T-piece cannula fistulas into the small intestine of the pig. Nihon Chikusan Gakkaiho 45: 42-44. https://doi.org/10.2508/chikusan.45.42
-
Gargallo, J. and Zimmermann, D.R., 1980. A simple intestinal cannula for swine. American Journal of Veterinary Research 41: 618-619.
'A simple intestinal cannula for swine ' () 41 American Journal of Veterinary Research : 618 -619.
-
Green, S., Bertrand, S.L., Duron, M.J.C. and Maillard, R.A., 1987. Digestibility of amino acids in maize, wheat and barley meal, measured in pigs with ileo-rectal anastomosis and isolation of the large intestine. Journal of the Science of Food and Agriculture 41: 29-43. https://doi.org/10.1002/jsfa.2740410104
-
Gutierrez, N.A., Serao, N.V.L. and Patience, J.F., 2016. Effects of distillers’ dried grains with solubles and soybean oil on dietary lipid, fiber, and amino acid digestibility in corn-based diets fed to growing pigs. Journal of Animal Science 94: 1508-1519. https://doi:10.2527/jas.2015-9529
-
Knabe, D.A., LaRue, D.C., Gregg, E.J., Martinez, G.M. and Tanksley, T.D., 1989. Apparent digestibility of nitrogen and amino acids in protein rich feedstuffs by growing pigs. Journal of Animal Science 67: 441-458. https://doi.org/10.2527/jas1989.672441x
-
Li, R., Hou, G.F., Song, Z.H., Zhao, J.F., Fan, Z.Y., Hou, D.X. and Hea, X., 2019. Nutritional value of enzyme-treated soybean meal, concentrated degossypolized cottonseed protein, dried porcine solubles and fish meal for 10- to 20 kg pigs. Animal Feed Science and Technology 252: 23-33. https://doi.org/10.1016/j.anifeedsci.2019.04.002
-
NRC, 2012. Nutrient requirements of swine. 11th revised edition. The National Academies Press, Washington, DC, USA, 400 pp.
-
Newman, M.A., Zebeli, Q., Velde, K., Grüll, D., Molnar, T., Kandler, W. and Metzler-Zebeli, B.U., 2016. Enzymatically modified starch favorably modulated intestinal transit time and hindgut fermentation in growing pigs. PloS ONE 11(12): e0167784. https://doi.org/10.1371/journal.pone.0167784
-
Stein, H.H., Shipley, C.F. and Easter, R.A., 1998. Technical note: a technique for inserting a T-cannula into the distal ileum of pregnant sows. Journal of Animal Science 76: 1433-1436. https://doi.org/10.2527/1998.7651433x
-
Swindle, M.M. and Smith, A.C., 2015. Swine in the laboratory: surgery, anesthesia, imaging, and experimental techniques. 3rd edition. CRC press. Boca Raton, FL, USA, 594 pp.
'Swine in the laboratory: surgery, anesthesia, imaging, and experimental techniques ', () 594.
-
Walker, W.R., Morgan, G.L. and Maxwell, C.V., 1986. Ileal cannulation in baby pigs with a simple T-cannula. Journal of Animal Science 62: 407-411. https://doi.org/10.2527/jas1986.622407x
-
Wubben, J.E., Smiricky, M.R., Albin, D.M. and Gabert, V.M., 2001. Improved procedure and cannula design for simple-T cannulation at the distal ileum in growing pigs. Laboratory Animal Science, 40: 27-31.
'Improved procedure and cannula design for simple-T cannulation at the distal ileum in growing pigs ' () 40 Laboratory Animal Science : 27 -31.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 0 | 0 | 0 |
| Gesamttextansichten | 327 | 136 | 20 |
| PDF-Downloads | 241 | 81 | 12 |
A procedure for dual simple t-cannulation in the small intestine of pigs using a single right flank laparotomy
in Journal of Applied Animal NutritionSearch for other papers by A.L. Petry in
Current site
Google Scholar
PubMed
Search for other papers by S.A. Gould in
Current site
Google Scholar
PubMed
Search for other papers by J.F. Patience in
Current site
Google Scholar
PubMed
Singular cannulation in the small intestine of pigs is a common methodology for studying nutrient digestibility, delivering compounds into the gastrointestinal tract, or repeated tissue and digesta sampling. In that respect, it is an important tool for nutritionists and researchers. However, there is a dearth of detailed methodologies describing multiple intestinal cannulations using modern techniques. The objective of this experiment was to develop a single right flank laparotomy technique that allowed for imsertion of multiple cannulas in the small intestine, with minimal variation, and allowed for externalisation of both cannulas on the same lateral side as the laparotomy. Thirty gilts (L337 × Camborough) with an initial body weight of 30.2±0.78 kg underwent the procedure. Each gilt was equipped with a simple t-cannula in the terminal ileum, approximately 10 cm cranial to the ileocecal valve, and a second t-cannula in the jejunum 240 cm distal from where the duodenum is visually posterior to the transverse colon. The procedure used hallmarks of commonly implemented terminal ileal cannulation techniques, but modified the laparotomy location and cannula externalisation sites and used a novel approach for determining the more proximal cannula location to mitigate the need of a second laparotomy. Gilts were allowed to recover for a minimum of 7 d and were used for an average of 67 days in subsequent experimental trials. Cannula longevity was from 30 to 73 kg of body weight. Pigs were necropsied for surgical site gross examination and small intestine measurements at the end of the experiment. The jejunal cannula had a mean placement of 298.90±9.96 cm distal to the pyloric sphincter with a coefficient of variation of 3.33%. Hence, this procedure provided a single laparotomy technique for obtaining digesta from multiple locations of the small intestine, with minimal variation in proximal cannula placement.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 0 | 0 | 0 |
| Gesamttextansichten | 327 | 136 | 20 |
| PDF-Downloads | 241 | 81 | 12 |
