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In vitro degradation of non-starch polysaccharide residues in the digesta of broilers offered wheat-soy or maize-soy diets by feed enzymes
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Abstract
The present study evaluated the in vitro efficacy of different feed enzymes on degrading non-starch polysaccharide (NSP) residues present in digesta collected from broilers’ gastrointestinal tract. Two dietary treatments, wheat-soy or maize-soy based diets, were assigned to broilers (12 replicates per treatment; 10 birds per replicate pen) from d 0 to 35. At 35 d of age, digesta was collected from the gizzard, jejunum and ileum from three birds per replicate and pooled. The digesta samples were digested in vitro with one of nine enzymes; GH10, GH11 and GH8 xylanase, arabinofuranosidase, a combination of GH10 xylanase and ß-glucanase (XYN+BG), ß-mannanase, protease, cellulase cocktail and pectate lyase. When using the gizzard digesta as substrate, GH8 xylanase reduced (P<0.005) both insoluble and soluble NSP levels compared to the control, regardless of diet type. Analysis of NSP degradation in jejunal digesta from birds fed the wheat-soy diet revealed that the three xylanases and arabinofuranosidase reduced (P<0.001) insoluble NSP level compared to the control, but the soluble NSP level was not affected by the enzyme treatments. All enzymes, except the cellulase cocktail and pectate lyase, reduced (P=0.005) the insoluble NSP level in the jejunal digesta collected from birds fed the maize-soy diet. Similarly, all enzyme preparations reduced (P<0.001) the insoluble NSP level in ileal digesta obtained from birds fed the maize-soy diet. The GH10 and GH11 xylanase, XYN+BG, cellulase cocktail and pectate lyase reduced (P<0.001) the insoluble NSP level in the ileal digesta from birds fed the wheat-soy diet compared to the control, with XYN+BG exhibiting the greatest reduction. Collectively, the present in vitro study indicated that the in vitro efficacy of enzyme preparations was dictated by the amount and type of NSP remaining undigested in various parts of the gut, which perhaps affect the in vivo efficacy.
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Abdollahi, M., Ravindran, V. and Svihus, B., 2013. Pelleting of broiler diets: an overview with emphasis on pellet quality and nutritional value. Animal Feed Science and Technology 179: 1-23.
-
Association of Official Agricultural Chemists (AOAC), 2012. Official methods of analysis. AOAC International, Gaithersburg, MD, USA.
-
Bach Knudsen, K.E., 2014. Fiber and nonstarch polysaccharide content and variation in common crops used in broiler diets. Poultry Science 93: 2380-2393.
-
Bedford, M., 1995. Mechanism of action and potential environmental benefits from the use of feed enzymes. Animal Feed Science and Technology 53: 145-155.
-
Bedford, M.R., 1996. The effect of enzymes on digestion. Journal of Applied Poultry Research 5: 370-378.
-
Caprita, R., Caprita, A. and Cretescu, I., 2011. Effect of extraction conditions on the solubility of non-starch polysaccharides of wheat and barley. Journal of Food, Agriculture and Environment 9: 132134.
-
Chaplin, M.F., 2003. Fibre and water binding. Proceedings of the Nutrition Society 62: 223-227.
-
Cobb-Vantress, 2018. Cobb 500 Broiler performance and nutrition supplement. Cobb, Siloam Springs, AR, USA.
-
Debroas, D. and Blanchart, G., 1993. Interactions between proteolytic and cellulolytic rumen bacteria during hydrolysis of plant cell wall protein. Reproduction Nutrition Development 33: 283-288.
-
Dombrink-Kurtzman, M. and Knutson, C., 1997. A study of maize endosperm hardness in relation to amylose content and susceptibility to damage. Cereal Chemistry 74: 776-780.
-
Englyst, H.N., Quigley, M.E. and Hudson, G.J., 1994. Determination of dietary fibre as non-starch polysaccharides with gas-liquid chromatographic, high-performance liquid chromatographic or spectrophotometric measurement of constituent sugars. Analyst 119: 1497-1509.
-
Hetland, H., Choct, M. and Svihus, B., 2004. Role of insoluble non-starch polysaccharides in poultry nutrition. World’s Poultry Science Journal 60: 415-422.
-
Hsiao, H.-Y., Anderson, D. and Dale, N., 2006. Levels of β-mannan in soybean meal. Poultry Science 85: 1430-1432.
-
Izydorczyk, M.S. and Biliaderis, C.G., 1995. Cereal arabinoxylans: advances in structure and physicochemical properties. Carbohydrate Polymers 28: 33-48.
-
Jozefiak, D., Rutkowski, A., Jensen, B. and Engberg, R., 2007. Effects of dietary inclusion of triticale, rye and wheat and xylanase supplementation on growth performance of broiler chickens and fermentation in the gastrointestinal tract. Animal Feed Science and Technology 132: 79-93.
-
Masey-O’Neill, H., Singh, M. and Cowieson, A., 2014. Effects of exogenous xylanase on performance, nutrient digestibility, volatile fatty acid production and digestive tract thermal profiles of broilers fed on wheat-or maize-based diet. British Poultry Science 55: 351-359.
-
Mathew, S. and Abraham, T.E., 2004. Ferulic acid: an antioxidant found naturally in plant cell walls and feruloyl esterases involved in its release and their applications. Critical Reviews in Biotechnology 24: 59-83.
-
Meng, X. and Slominski, B., 2005. Nutritive values of corn, soybean meal, canola meal, and peas for broiler chickens as affected by a multicarbohydrase preparation of cell wall degrading enzymes. Poultry Science 84: 1242-1251.
-
Morgan, N.K., Keerqin, C., Wallace, A., Wu, S.-B. and Choct, M., 2019. Effect of arabinoxylo-oligosaccharides and arabinoxylans on net energy and nutrient utilization in broilers. Animal Nutrition 5: 56-62.
-
Nguyen, H.T., Bedford, M.R., Wu, S.-B. and Morgan, N.K., 2021. Soluble non-starch polysaccharide modulates broiler gastrointestinal tract environment. Poultry Science 100(8): 101183.
-
Olukosi, O., Beeson, L., Englyst, K. and Romero, L., 2015. Effects of exogenous proteases without or with carbohydrases on nutrient digestibility and disappearance of non-starch polysaccharides in broiler chickens. Poultry Science 94: 2662-2669.
-
Rodrigues, I. and Choct, M., 2018. The foregut and its manipulation via feeding practices in the chicken. Poultry Science 97: 3188-3206.
-
Rose, D.J., Patterson, J.A. and Hamaker, B.R., 2010. Structural differences among alkali-soluble arabinoxylans from maize (Zea mays), rice (Oryza sativa), and wheat (Triticum aestivum) brans influence human fecal fermentation profiles. Journal of Agricultural and Food Chemistry 58: 493-499.
-
Rowe, J., Choct, M. and Pethick, D., 1999. Processing cereal grains for animal feeding. Australian Journal of Agricultural Research 50: 721-736.
-
Saha, B.C., 2000. α-L-Arabinofuranosidases: biochemistry, molecular biology and application in biotechnology. Biotechnology Advances 18: 403-423.
-
Scott, R.W., 1979. Colorimetric determination of hexuronic acids in plant materials. Analytical Chemistry 51: 936-941.
-
Smits, C.H. and Annison, G., 1996. Non-starch plant polysaccharides in broiler nutrition – towards a physiologically valid approach to their determination. World’s Poultry Science Journal 52: 203-221.
-
Svihus, B. and Itani, K., 2019. Intestinal passage and its relation to digestive processes. Journal of Applied Poultry Research 28: 546-555.
-
Svihus, B., 2011. Effect of digestive tract conditions, feed processing and ingredients on response to NSP enzymes. In: Bedford, M.R. and Partridge, G.G. (eds.) Enzymes in farm animal nutrition. CABI, Wallingford, UK, pp. 129-159.
-
Theander, O., Åman, P., Westerlund, E., Andersson, R. and Pettersson, D., 1995. Total dietary fiber determined as neutral sugar residues, uronic acid residues, and Klason lignin (the Uppsala method): collaborative study. Journal of AOAC International 78: 1030-1044.
-
Yadav, S., Yadav, P.K., Yadav, D. and Yadav, K.D.S., 2009. Pectin lyase: a review. Process Biochemistry 44: 1-10.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 0 | 0 | 0 |
| Gesamttextansichten | 500 | 191 | 21 |
| PDF-Downloads | 319 | 98 | 7 |
In vitro degradation of non-starch polysaccharide residues in the digesta of broilers offered wheat-soy or maize-soy diets by feed enzymes
in Journal of Applied Animal NutritionSearch for other papers by E. Kim in
Current site
Google Scholar
PubMed
Search for other papers by N.K. Morgan in
Current site
Google Scholar
PubMed
Search for other papers by A.F. Moss in
Current site
Google Scholar
PubMed
Search for other papers by A. Solbak in
Current site
Google Scholar
PubMed
Search for other papers by L. Li in
Current site
Google Scholar
PubMed
Search for other papers by P. Ader in
Current site
Google Scholar
PubMed
Search for other papers by M. Choct in
Current site
Google Scholar
PubMed
Abstract
The present study evaluated the in vitro efficacy of different feed enzymes on degrading non-starch polysaccharide (NSP) residues present in digesta collected from broilers’ gastrointestinal tract. Two dietary treatments, wheat-soy or maize-soy based diets, were assigned to broilers (12 replicates per treatment; 10 birds per replicate pen) from d 0 to 35. At 35 d of age, digesta was collected from the gizzard, jejunum and ileum from three birds per replicate and pooled. The digesta samples were digested in vitro with one of nine enzymes; GH10, GH11 and GH8 xylanase, arabinofuranosidase, a combination of GH10 xylanase and ß-glucanase (XYN+BG), ß-mannanase, protease, cellulase cocktail and pectate lyase. When using the gizzard digesta as substrate, GH8 xylanase reduced (P<0.005) both insoluble and soluble NSP levels compared to the control, regardless of diet type. Analysis of NSP degradation in jejunal digesta from birds fed the wheat-soy diet revealed that the three xylanases and arabinofuranosidase reduced (P<0.001) insoluble NSP level compared to the control, but the soluble NSP level was not affected by the enzyme treatments. All enzymes, except the cellulase cocktail and pectate lyase, reduced (P=0.005) the insoluble NSP level in the jejunal digesta collected from birds fed the maize-soy diet. Similarly, all enzyme preparations reduced (P<0.001) the insoluble NSP level in ileal digesta obtained from birds fed the maize-soy diet. The GH10 and GH11 xylanase, XYN+BG, cellulase cocktail and pectate lyase reduced (P<0.001) the insoluble NSP level in the ileal digesta from birds fed the wheat-soy diet compared to the control, with XYN+BG exhibiting the greatest reduction. Collectively, the present in vitro study indicated that the in vitro efficacy of enzyme preparations was dictated by the amount and type of NSP remaining undigested in various parts of the gut, which perhaps affect the in vivo efficacy.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 0 | 0 | 0 |
| Gesamttextansichten | 500 | 191 | 21 |
| PDF-Downloads | 319 | 98 | 7 |
