The application of in vitro human intestinal models on the screening and development of pre- and probiotics
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The importance of the gut microbiota community on host’s health and disease has long been recognised and is well documented. The development of pro- and prebiotic interventions offers an opportunity for the modulation of the gut microbiota towards long lasting health. In vitro fermentation models were developed as a powerful tool to study the impact of pro- and prebiotics on the gut microbiota under tightly controlled conditions, which allow dynamic sampling over time in reactors mimicking different colon regions. These models have been further evolved to suit specific experimental purposes, e.g. including immobilised faecal microbiota, peristaltic movement, mucin microcosm and the ability to perform treatments in parallel. In this review we discuss the advantages, disadvantages and technical considerations of the most frequently used models. We further focus on recent advances in the application of these models in prebiotics and probiotics research and outline their predictability for clinical research.
-
Aguirre, M., Jonkers, D.M., Troost, F.J., Roeselers, G. and Venema, K., 2014a. In vitro characterization of the impact of different substrates on metabolite production, energy extraction and composition of gut microbiota from lean and obese subjects. PLoS ONE 9: e113864.
'In vitro characterization of the impact of different substrates on metabolite production, energy extraction and composition of gut microbiota from lean and obese subjects ' () 9 PLoS ONE : e113864.
-
Aguirre, M., Ramiro-Garcia, J., Koenen, M.E. and Venema, K., 2014b. To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies. Journal of Microbiological Methods 107: 1-7.
'To pool or not to pool? ' () 107 Journal of Microbiological Methods : 1 -7.
-
Aguirre, M. and Venema, K., 2017. Challenges in simulating the human gut for understanding the role of the microbiota in obesity. Beneficial Microbes 8: 31-53.
'Challenges in simulating the human gut for understanding the role of the microbiota in obesity ' () 8 Beneficial Microbes : 31 -53.
-
Al-Tamimi, M.A., Palframan, R.J., Cooper, J.M., Gibson, G.R. and Rastall, R.A., 2006. In vitro fermentation of sugar beet arabinan and arabino-oligosaccharides by the human gut microflora. Journal of Applied Microbiology 100: 407-414.
'In vitro fermentation of sugar beet arabinan and arabino-oligosaccharides by the human gut microflora ' () 100 Journal of Applied Microbiology : 407 -414.
-
Alander, M., De Smet, I., Nollet, L., Verstraete, W., Von Wright, A. and Mattila-Sandholm, T., 1999. The effect of probiotic strains on the microbiota of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME). International Journal of Food Microbiology 46: 71-79.
'The effect of probiotic strains on the microbiota of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) ' () 46 International Journal of Food Microbiology : 71 -79.
-
Alizadeh, A., Akbari, P., Difilippo, E., Schols, H.A., Ulfman, L.H., Schoterman, M.H.C., Garssen, J., Fink-Gremmels, J. and Braber, S., 2016. The piglet as a model for studying dietary components in infant diets: effects of galacto-oligosaccharides on intestinal functions. British Journal of Nutrition 115: 605-618.
'The piglet as a model for studying dietary components in infant diets: effects of galacto-oligosaccharides on intestinal functions ' () 115 British Journal of Nutrition : 605 -618.
-
Ambalam, P., Raman, M., Purama, R.K. and Doble, M., 2016. Probiotics, prebiotics and colorectal cancer prevention. Best Practice and Research: Clinical Gastroenterology 30: 119-131.
'Probiotics, prebiotics and colorectal cancer prevention ' () 30 Best Practice and Research: Clinical Gastroenterology : 119 -131.
-
Barroso, E., Cueva, C., Pelaez, C., Martínez-Cuesta, M.C. and Requena, T., 2015. Development of human colonic microbiota in the computer-controlled dynamic SIMulator of the GastroIntestinal tract SIMGI. Food Science and Technology Bulletin: Functional Foods 61: 283-289.
'Development of human colonic microbiota in the computer-controlled dynamic SIMulator of the GastroIntestinal tract SIMGI ' () 61 Food Science and Technology Bulletin: Functional Foods : 283 -289.
-
Beards, E., Tuohy, K. and Gibson, G., 2010. Bacterial, SCFA and gas profiles of a range of food ingredients following in vitro fermentation by human colonic microbiota. Anaerobe 16: 420-425.
'Bacterial, SCFA and gas profiles of a range of food ingredients following in vitro fermentation by human colonic microbiota ' () 16 Anaerobe : 420 -425.
-
Belenguer, A., Duncan, S.H., Holtrop, G., Anderson, S.E., Lobley, G.E. and Flint, H.J., 2007. Impact of pH on lactate formation and utilization by human fecal microbial communities. Applied and Environmental Microbiology 73: 6526-6533.
'Impact of pH on lactate formation and utilization by human fecal microbial communities ' () 73 Applied and Environmental Microbiology : 6526 -6533.
-
Bermudez-Brito, M., Plaza-Diaz, J., Munoz-Quezada, S., Gomez-Llorente, C. and Gil, A., 2012. Probiotic mechanisms of action. Annals of Nutrition and Metabolism 61: 160-174.
'Probiotic mechanisms of action ' () 61 Annals of Nutrition and Metabolism : 160 -174.
-
Bindels, L.B., Delzenne, N.M., Cani, P.D. and Walter, J., 2015. Towards a more comprehensive concept for prebiotics. Nature Reviews Gastroenterology and Hepatology 12: 303-310.
'Towards a more comprehensive concept for prebiotics ' () 12 Nature Reviews Gastroenterology and Hepatology : 303 -310.
-
Cardelle-Cobas, A., Olano, A., Corzo, N., Villamiel, M., Collins, M., Kolida, S. and Rastall, R.A., 2012. In vitro fermentation of lactulose-derived oligosaccharides by mixed fecal microbiota. Journal of Agricultural and Food Chemistry 60: 2024-2032.
'In vitro fermentation of lactulose-derived oligosaccharides by mixed fecal microbiota ' () 60 Journal of Agricultural and Food Chemistry : 2024 -2032.
-
Carlson, J. and Slavin, J., 2016. Health benefits of fibre, prebiotics and probiotics: a review of intestinal health and related health claims. Quality Assurance and Safety of Crops & Foods 8: 539-553.
'Health benefits of fibre, prebiotics and probiotics: a review of intestinal health and related health claims ' () 8 Quality Assurance and Safety of Crops & Foods : 539 -553.
-
Chaikham, P., Apichartsrangkoon, A., Jirarattanarangsri, W. and Van de Wiele, T., 2012. Influence of encapsulated probiotics combined with pressurized longan juice on colon microflora and their metabolic activities on the exposure to simulated dynamic gastrointestinal tract. Food Research International 49: 133-142.
'Influence of encapsulated probiotics combined with pressurized longan juice on colon microflora and their metabolic activities on the exposure to simulated dynamic gastrointestinal tract ' () 49 Food Research International : 133 -142.
-
Charbonneau, M.R., O’Donnell, D., Blanton, L.V., Totten, S.M., Davis, J.C., Barratt, M.J., Cheng, J., Guruge, J., Talcott, M., Bain, J.R., Muehlbauer, M.J., Ilkayeva, O., Wu, C., Struckmeyer, T., Barile, D., Mangani, C., Jorgensen, J., Fan, Y.M., Maleta, K., Dewey, K.G., Ashorn, P., Newgard, C.B., Lebrilla, C., Mills, D.A. and Gordon, J.I., 2016. Sialylated milk oligosaccharides promote microbiota-dependent growth in models of infant undernutrition. Cell 164: 859-871.
'Sialylated milk oligosaccharides promote microbiota-dependent growth in models of infant undernutrition ' () 164 Cell : 859 -871.
-
Child, M.W., Kennedy, A., Walker, A.W., Bahrami, B., MacFarlane, S. and MacFarlane, G.T., 2006. Studies on the effect of system retention time on bacterial populations colonizing a three-stage continuous culture model of the human large gut using FISH techniques. FEMS Microbiology Ecology 55: 299-310.
'Studies on the effect of system retention time on bacterial populations colonizing a three-stage continuous culture model of the human large gut using FISH techniques ' () 55 FEMS Microbiology Ecology : 299 -310.
-
Chung, W.S.F., Walker, A.W., Louis, P., Parkhill, J., Vermeiren, J., Bosscher, D., Duncan, S.H. and Flint, H.J., 2016. Modulation of the human gut microbiota by dietary fibres occurs at the species level. BMC Biology 14: 3.
'Modulation of the human gut microbiota by dietary fibres occurs at the species level ' () 14 BMC Biology : 3.
-
Cinquin, C., Le Blay, G., Fliss, I. and Lacroix, C., 2004. Immobilization of infant fecal microbiota and utilization in an in vitro colonic fermentation model. Microbial Ecology 48: 128-138.
'Immobilization of infant fecal microbiota and utilization in an in vitro colonic fermentation model ' () 48 Microbial Ecology : 128 -138.
-
Cinquin, C., Le Blay, G., Fliss, I. and Lacroix, C., 2006. Comparative effects of exopolysaccharides from lactic acid bacteria and fructo-oligosaccharides on infant gut microbiota tested in an in vitro colonic model with immobilized cells. FEMS Microbiology Ecology 57: 226-238.
'Comparative effects of exopolysaccharides from lactic acid bacteria and fructo-oligosaccharides on infant gut microbiota tested in an in vitro colonic model with immobilized cells ' () 57 FEMS Microbiology Ecology : 226 -238.
-
Clark, M.J., Robien, K. and Slavin, J.L., 2012. Effect of prebiotics on biomarkers of colorectal cancer in humans: a systematic review. Nutrition Reviews 70: 436-443.
'Effect of prebiotics on biomarkers of colorectal cancer in humans: a systematic review ' () 70 Nutrition Reviews : 436 -443.
-
Cohen, S.M., 1995. Human relevance of animal carcinogenicity studies. Regulatory Toxicology and Pharmacology 21: 75-80.
'Human relevance of animal carcinogenicity studies ' () 21 Regulatory Toxicology and Pharmacology : 75 -80.
-
Costabile, A., Walton, G.E., Tzortzis, G., Vulevic, J., Charalampopoulos, D. and Gibson, G.R., 2015. Effects of orange juice formulation on prebiotic functionality using an in vitro colonic model system. PLoS ONE 10: e0121955.
'Effects of orange juice formulation on prebiotic functionality using an in vitro colonic model system ' () 10 PLoS ONE : e0121955.
-
Cummings, J.H. and MacFarlane, G.T., 1991. The control and consequences of bacterial fermentation in the human colon. Journal of Applied Bacteriology 70: 443-459.
'The control and consequences of bacterial fermentation in the human colon ' () 70 Journal of Applied Bacteriology : 443 -459.
-
Daguet, D., Pinheiro, I., Verhelst, A., Possemiers, S. and Marzorati, M., 2016. Arabinogalactan and fructooligosaccharides improve the gut barrier function in distinct areas of the colon in the Simulator of the Human Intestinal Microbial Ecosystem. Journal of Functional Foods 20: 369-379.
'Arabinogalactan and fructooligosaccharides improve the gut barrier function in distinct areas of the colon in the Simulator of the Human Intestinal Microbial Ecosystem ' () 20 Journal of Functional Foods : 369 -379.
-
Dahiya, D.K., Renuka, Puniya, M., Shandilya, U.K., Dhewa, T., Kumar, N., Kumar, S., Puniya, A.K. and Shukla, P., 2017. Gut microbiota modulation and its relationship with obesity using prebiotic fibers and probiotics: a review. Frontiers in Microbiology 8: 563.
'Gut microbiota modulation and its relationship with obesity using prebiotic fibers and probiotics: a review ' () 8 Frontiers in Microbiology : 563.
-
David, L.A., Maurice, C.F., Carmody, R.N., Gootenberg, D.B., Button, J.E., Wolfe, B.E., Ling, A.V., Devlin, A.S., Varma, Y., Fischbach, M.A., Biddinger, S.B., Dutton, R.J. and Turnbaugh, P.J., 2014. Diet rapidly and reproducibly alters the human gut microbiome. Nature 505: 559-563.
'Diet rapidly and reproducibly alters the human gut microbiome ' () 505 Nature : 559 -563.
-
Donaldson, G.P., Lee, S.M. and Mazmanian, S.K., 2016. Gut biogeography of the bacterial microbiota. Nature Reviews Microbiology 14: 20-32.
'Gut biogeography of the bacterial microbiota ' () 14 Nature Reviews Microbiology : 20 -32.
-
Dostal, A., Fehlbaum, S., Chassard, C., Zimmermann, M.B. and Lacroix, C., 2012. Low iron availability in continuous in vitro colonic fermentations induces strong dysbiosis of the child gut microbial consortium and a decrease in main metabolites. FEMS Microbiology Ecology 83: 161-175.
'Low iron availability in continuous in vitro colonic fermentations induces strong dysbiosis of the child gut microbial consortium and a decrease in main metabolites ' () 83 FEMS Microbiology Ecology : 161 -175.
-
Duncan, S.H., Louis, P., Thomson, J.M. and Flint, H.J., 2009. The role of pH in determining the species composition of the human colonic microbiota. Environmental Microbiology 11: 2112-2122.
'The role of pH in determining the species composition of the human colonic microbiota ' () 11 Environmental Microbiology : 2112 -2122.
-
Eckburg, P.B., Bik, E.M., Bernstein, C.N., Purdom, E., Dethlefsen, L., Sargent, M., Gill, S.R., Nelson, K.E. and Relman, D.A., 2005. Diversity of the human intestinal microbial flora. Science 308: 1635-1638.
'Diversity of the human intestinal microbial flora ' () 308 Science : 1635 -1638.
-
Fassler, C., Arrigoni, E., Venema, K., Brouns, F. and Amado, R., 2006. In vitro fermentability of differently digested resistant starch preparations. Molecular Nutrition and Food Research 50: 1220-1228.
'In vitro fermentability of differently digested resistant starch preparations ' () 50 Molecular Nutrition and Food Research : 1220 -1228.
-
Fehlbaum, S., Chassard, C., Haug, M.C., Fourmestraux, C., Derrien, M. and Lacroix, C., 2015. Design and investigation of polyferms in vitro continuous fermentation models inoculated with immobilized fecal microbiota mimicking the elderly colon. PLoS ONE 10: e0142793.
'Design and investigation of polyferms in vitro continuous fermentation models inoculated with immobilized fecal microbiota mimicking the elderly colon ' () 10 PLoS ONE : e0142793.
-
Fooks, L.J. and Gibson, G.R., 2003. Mixed culture fermentation studies on the effects of synbiotics on the human intestinal pathogens Campylobacter jejuni and Escherichia coli. Anaerobe 9: 231-242.
'Mixed culture fermentation studies on the effects of synbiotics on the human intestinal pathogens Campylobacter jejuni and Escherichia coli ' () 9 Anaerobe : 231 -242.
-
Ghouri, Y.A., Richards, D.M., Rahimi, E.F., Krill, J.T., Jelinek, K.A. and DuPont, A.W., 2014. Systematic review of randomized controlled trials of probiotics, prebiotics, and synbiotics in inflammatory bowel disease. Clinical and Experimental Gastroenterology 7: 473-487.
'Systematic review of randomized controlled trials of probiotics, prebiotics, and synbiotics in inflammatory bowel disease ' () 7 Clinical and Experimental Gastroenterology : 473 -487.
-
Gibson, G.R., Cummings, J.H. and MacFarlane, G.T., 1988. Use of a three-stage continuous culture system to study the effect of mucin on dissimilatory sulfate reduction and methanogenesis by mixed populations of human gut bacteria. Applied and Environmental Microbiology 54: 2750-2755.
'Use of a three-stage continuous culture system to study the effect of mucin on dissimilatory sulfate reduction and methanogenesis by mixed populations of human gut bacteria ' () 54 Applied and Environmental Microbiology : 2750 -2755.
-
Gibson, G.R., Hutkins, R., Sanders, M.E., Prescott, S.L., Reimer, R.A., Salminen, S.J., Scott, K., Stanton, C., Swanson, K.S., Cani, P.D., Verbeke, K. and Reid, G., 2017. Expert consensus document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nature Reviews Gastroenterology and Hepatology 14: 491-502.
'Expert consensus document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics ' () 14 Nature Reviews Gastroenterology and Hepatology : 491 -502.
-
Gibson, G.R. and Roberfroid, M.B., 1995. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. Journal of Nutrition 125: 1401-1412.
'Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics ' () 125 Journal of Nutrition : 1401 -1412.
-
Goulet, O., 2015. Potential role of the intestinal microbiota in programming health and disease. Nutrition Reviews 73, Suppl. 1: 32-40.
'Potential role of the intestinal microbiota in programming health and disease ' () 73 Nutrition Reviews : 32 -40.
-
Grimaldi, R., Cela, D., Swann, J.R., Vulevic, J., Gibson, G.R., Tzortzis, G. and Costabile, A., 2017. In vitro fermentation of B-GOS: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children. FEMS Microbiology Ecology 93(2): 233.
'In vitro fermentation of B-GOS: impact on faecal bacterial populations and metabolic activity in autistic and non-autistic children ' () 93 FEMS Microbiology Ecology : 233.
-
Grootaert, C., Van den Abbeele, P., Marzorati, M., Broekaert, W.F., Courtin, C.M., Delcour, J.A., Verstraete, W. and Van de Wiele, T., 2009. Comparison of prebiotic effects of arabinoxylan oligosaccharides and inulin in a simulator of the human intestinal microbial ecosystem. FEMS Microbiology Ecology 69: 231-242.
'Comparison of prebiotic effects of arabinoxylan oligosaccharides and inulin in a simulator of the human intestinal microbial ecosystem ' () 69 FEMS Microbiology Ecology : 231 -242.
-
Grover, M. and Kashyap, P.C., 2014. Germ-free mice as a model to study effect of gut microbiota on host physiology. Neurogastroenterology and Motility 26: 745-748.
'Germ-free mice as a model to study effect of gut microbiota on host physiology ' () 26 Neurogastroenterology and Motility : 745 -748.
-
Guarner, F., 2006. Enteric flora in health and disease. Digestion 73, Suppl. 1: 5-12.
'Enteric flora in health and disease ' () 73 Digestion : 5 -12.
-
Henry, O.Y.F., Villenave, R., Cronce, M.J., Leineweber, W.D., Benz, M.A. and Ingber, D.E., 2017. Organs-on-chips with integrated electrodes for trans-epithelial electrical resistance (TEER) measurements of human epithelial barrier function. Lab on a Chip 17: 2264-2271.
'Organs-on-chips with integrated electrodes for trans-epithelial electrical resistance (TEER) measurements of human epithelial barrier function ' () 17 Lab on a Chip : 2264 -2271.
-
Hobden, M.R., Martin-Morales, A., Guerin-Deremaux, L., Wils, D., Costabile, A., Walton, G.E., Rowland, I., Kennedy, O.B. and Gibson, G.R., 2013. In vitro fermentation of NUTRIOSE((R)) FB06, a wheat dextrin soluble fibre, in a continuous culture human colonic model system. PLoS ONE 8: e77128.
'In vitro fermentation of NUTRIOSE((R)) FB06, a wheat dextrin soluble fibre, in a continuous culture human colonic model system ' () 8 PLoS ONE : e77128.
-
Hughes, S.A., Shewry, P.R., Gibson, G.R., McCleary, B.V. and Rastall, R.A., 2008. In vitro fermentation of oat and barley derived beta-glucans by human faecal microbiota. FEMS Microbiology Ecology 64: 482-493.
'In vitro fermentation of oat and barley derived beta-glucans by human faecal microbiota ' () 64 FEMS Microbiology Ecology : 482 -493.
-
Hughes, S.A., Shewry, P.R., Li, L., Gibson, G.R., Sanz, M.L. and Rastall, R.A., 2007. In vitro fermentation by human fecal microflora of wheat arabinoxylans. Journal of Agricultural and Food Chemistry 55: 4589-4595.
'In vitro fermentation by human fecal microflora of wheat arabinoxylans ' () 55 Journal of Agricultural and Food Chemistry : 4589 -4595.
-
Hutkins, R.W., Krumbeck, J.A., Bindels, L.B., Cani, P.D., Fahey Jr., G., Goh, Y.J., Hamaker, B., Martens, E.C., Mills, D.A., Rastal, R.A., Vaughan, E. and Sanders, M.E., 2016. Prebiotics: why definitions matter. Current Opinion in Biotechnology 37: 1-7.
'Prebiotics: why definitions matter ' () 37 Current Opinion in Biotechnology : 1 -7.
-
Johnson, L.P., Walton, G.E., Psichas, A., Frost, G.S., Gibson, G.R. and Barraclough, T.G., 2015. Prebiotics modulate the effects of antibiotics on gut microbial diversity and functioning in vitro. Nutrients 7: 4480-4497.
'Prebiotics modulate the effects of antibiotics on gut microbial diversity and functioning in vitro ' () 7 Nutrients : 4480 -4497.
-
Kim, H.J., Huh, D., Hamilton, G. and Ingber, D.E., 2012. Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow. Lab on a Chip 12: 2165-2174.
'Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow ' () 12 Lab on a Chip : 2165 -2174.
-
Kostic, A.D., Howitt, M.R. and Garrett, W.S., 2013. Exploring host-microbiota interactions in animal models and humans. Genes and Development 27: 701-718.
'Exploring host-microbiota interactions in animal models and humans ' () 27 Genes and Development : 701 -718.
-
La Fata, G., Weber, P. and Mohajeri, M.H., 2017. Probiotics and the gut immune system: indirect regulation. Probiotics and Antimicrobial Proteins 10: 11-21.
'Probiotics and the gut immune system: indirect regulation ' () 10 Probiotics and Antimicrobial Proteins : 11 -21.
-
Lacroix, C., De Wouters, T. and Chassard, C., 2015. Integrated multi-scale strategies to investigate nutritional compounds and their effect on the gut microbiota. Current Opinion in Biotechnology 32: 149-155.
'Integrated multi-scale strategies to investigate nutritional compounds and their effect on the gut microbiota ' () 32 Current Opinion in Biotechnology : 149 -155.
-
Ladirat, S.E., Schoterman, M.H., Rahaoui, H., Mars, M., Schuren, F.H., Gruppen, H., Nauta, A. and Schols, H.A., 2014. Exploring the effects of galacto-oligosaccharides on the gut microbiota of healthy adults receiving amoxicillin treatment. British Journal of Nutrition 112: 536-546.
'Exploring the effects of galacto-oligosaccharides on the gut microbiota of healthy adults receiving amoxicillin treatment ' () 112 British Journal of Nutrition : 536 -546.
-
Le Blay, G., Chassard, C., Baltzer, S. and Lacroix, C., 2010. Set up of a new in vitro model to study dietary fructans fermentation in formula-fed babies. British Journal of Nutrition 103: 403-411.
'Set up of a new in vitro model to study dietary fructans fermentation in formula-fed babies ' () 103 British Journal of Nutrition : 403 -411.
-
Likotrafiti, E., Tuohy, K.M., Gibson, G.R. and Rastall, R.A., 2014. An in vitro study of the effect of probiotics, prebiotics and synbiotics on the elderly faecal microbiota. Anaerobe 27: 50-55.
'An in vitro study of the effect of probiotics, prebiotics and synbiotics on the elderly faecal microbiota ' () 27 Anaerobe : 50 -55.
-
Liu, Y., Gibson, G.R. and Walton, G.E., 2016. An in vitro approach to study effects of prebiotics and probiotics on the faecal microbiota and selected immune parameters relevant to the elderly. PLoS ONE 11: e0162604.
'An in vitro approach to study effects of prebiotics and probiotics on the faecal microbiota and selected immune parameters relevant to the elderly ' () 11 PLoS ONE : e0162604.
-
Maathuis, A.J., Van den Heuvel, E.G. Schoterman, M.H. and Venema, K., 2012. Galacto-oligosaccharides have prebiotic activity in a dynamic in vitro colon model using a (13)C-labeling technique. Journal of Nutrition 142: 1205-1212.
'Galacto-oligosaccharides have prebiotic activity in a dynamic in vitro colon model using a (13)C-labeling technique ' () 142 Journal of Nutrition : 1205 -1212.
-
MacFarlane, G.T., Cummings, J.H., MacFarlane, S. and Gibson, G.R., 1989. Influence of retention time on degradation of pancreatic enzymes by human colonic bacteria grown in a 3-stage continuous culture system. Journal of Applied Bacteriology 67: 520-527.
'Influence of retention time on degradation of pancreatic enzymes by human colonic bacteria grown in a 3-stage continuous culture system ' () 67 Journal of Applied Bacteriology : 520 -527.
-
MacFarlane, G.T., Gibson, G.R. and Cummings, J.H., 1992. Comparison of fermentation reactions in different regions of the human colon. Journal of Applied Bacteriology 72: 57-64.
'Comparison of fermentation reactions in different regions of the human colon ' () 72 Journal of Applied Bacteriology : 57 -64.
-
MacFarlane, G.T. and MacFarlane, S., 2007. Models for intestinal fermentation: association between food components, delivery systems, bioavailability and functional interactions in the gut. Current Opinion in Biotechnology 18: 156-162.
'Models for intestinal fermentation: association between food components, delivery systems, bioavailability and functional interactions in the gut ' () 18 Current Opinion in Biotechnology : 156 -162.
-
MacFarlane, G.T., MacFarlane, S. and Gibson, G.R., 1998. Validation of a three-stage compound continuous culture system for investigating the effect of retention time on the ecology and metabolism of bacteria in the human colon. Microbial Ecology 35: 180-187.
'Validation of a three-stage compound continuous culture system for investigating the effect of retention time on the ecology and metabolism of bacteria in the human colon ' () 35 Microbial Ecology : 180 -187.
-
MacFarlane, S., Woodmansey, E.J. and MacFarlane, G.T., 2005. Colonization of mucin by human intestinal bacteria and establishment of biofilm communities in a two-stage continuous culture system. Applied and Environmental Microbiology 71: 7483-7492.
'Colonization of mucin by human intestinal bacteria and establishment of biofilm communities in a two-stage continuous culture system ' () 71 Applied and Environmental Microbiology : 7483 -7492.
-
Mäkivuokko, H. and Nurminen, P., 2006. In vitro methods to model the gastrointestinal tract. In: Ouwehand, A.C. and Vaughan, E.E. (eds.) Gastrointestinal microbiology. Taylor and Francis, New York, NY, USA, pp. 237-252.
'In vitro methods to model the gastrointestinal tract ', in Gastrointestinal microbiology , () 237 -252.
-
Martinez, R.C., Cardarelli, H.R., Borst, W., Albrecht, S., Schols, H., Gutierrez, O.P., Maathuis, A.J., De Melo Franco, B.D., De Martinis, E.C., Zoetendal, E.G. Venema, K., Saad, S.M. and Smidt, H., 2013. Effect of galactooligosaccharides and Bifidobacterium animalis Bb-12 on growth of Lactobacillus amylovorus DSM 16698, microbial community structure, and metabolite production in an in vitro colonic model set up with human or pig microbiota. FEMS Microbiology Ecology 84: 110-123.
'Effect of galactooligosaccharides and Bifidobacterium animalis Bb-12 on growth of Lactobacillus amylovorus DSM 16698, microbial community structure, and metabolite production in an in vitro colonic model set up with human or pig microbiota ' () 84 FEMS Microbiology Ecology : 110 -123.
-
Marzorati, M., Qin, B., Hildebrand, F., Klosterbuer, A., Roughead, Z., Roessle, C., Rochat, F., Raes, J. and Possemiers, S., 2015. Addition of acacia gum to a FOS/inulin blend improves its fermentation profile in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®). Journal of Functional Foods 16: 211-222.
'Addition of acacia gum to a FOS/inulin blend improves its fermentation profile in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) ' () 16 Journal of Functional Foods : 211 -222.
-
Marzorati, M., Vanhoecke, B., De Ryck, T., Sadaghian Sadabad, M., Pinheiro, I., Possemiers, S., Van den Abbeele, P., Derycke, L., Bracke, M., Pieters, J., Hennebel, T., Harmsen, H.J., Verstraete, W. and Van de Wiele, T., 2014. The HMI module: a new tool to study the Host-Microbiota Interaction in the human gastrointestinal tract in vitro. BMC Microbiology 14: 133.
'The HMI module: a new tool to study the Host-Microbiota Interaction in the human gastrointestinal tract in vitro ' () 14 BMC Microbiology : 133.
-
Marzorati, M., Verhelst, A., Luta, G., Sinnott, R., Verstraete, W., Van de Wiele, T. and Possemiers, S., 2010. In vitro modulation of the human gastrointestinal microbial community by plant-derived polysaccharide-rich dietary supplements. International Journal of Food Microbiology 139: 168-176.
'In vitro modulation of the human gastrointestinal microbial community by plant-derived polysaccharide-rich dietary supplements ' () 139 International Journal of Food Microbiology : 168 -176.
-
McDonald, J.A., Fuentes, S., Schroeter, K., Heikamp-DeJong, I., Khursigara, C.M., De Vos, W.M. and Allen-Vercoe, E., 2015. Simulating distal gut mucosal and luminal communities using packed-column biofilm reactors and an in vitro chemostat model. Journal of Microbiological Methods 108: 36-44.
'Simulating distal gut mucosal and luminal communities using packed-column biofilm reactors and an in vitro chemostat model ' () 108 Journal of Microbiological Methods : 36 -44.
-
Minekus, M., Smeets-Peeters, M., Bernalier, A., Marol-Bonnin, S., Havenaar, R., Marteau, P., Alric, M., Fonty, G. and Huis in’t Veld, J.H., 1999. A computer-controlled system to simulate conditions of the large intestine with peristaltic mixing, water absorption and absorption of fermentation products. Applied Microbiology and Biotechnology 53: 108-114.
'A computer-controlled system to simulate conditions of the large intestine with peristaltic mixing, water absorption and absorption of fermentation products ' () 53 Applied Microbiology and Biotechnology : 108 -114.
-
Mohajeri, H., La Fata, G., Steinert, R. and Weber, P., Relationship between gut microbiome and brain function. Nutrition Reviews 76: 481-496.
'Relationship between gut microbiome and brain function ' () 76 Nutrition Reviews : 481 -496.
-
Molly, K., De Smet, I., Nollet, L., Vande Woestyne, M. and Verstraete, W., 1996. Effect of Lactobacilli on the ecology of the gastro-intestinal microbiota cultured in the SHIME reactor. Microbial Ecology in Health and Disease 9: 79-89.
'Effect of Lactobacilli on the ecology of the gastro-intestinal microbiota cultured in the SHIME reactor ' () 9 Microbial Ecology in Health and Disease : 79 -89.
-
Molly, K., Vande Woestyne, M., De Smet, I. and Verstraete, W., 1994. Validation of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) reactor using microorganism-associated activities. Microbial Ecology in Health and Disease 7: 191-200.
'Validation of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) reactor using microorganism-associated activities ' () 7 Microbial Ecology in Health and Disease : 191 -200.
-
Moniz, P., Ho, A.L., Duarte, L.C., Kolida, S., Rastall, R.A., Pereira, H. and Carvalheiro, F., 2016. Assessment of the bifidogenic effect of substituted xylo-oligosaccharides obtained from corn straw. Carbohydrate Polymers 136: 466-473.
'Assessment of the bifidogenic effect of substituted xylo-oligosaccharides obtained from corn straw ' () 136 Carbohydrate Polymers : 466 -473.
-
Palframan, R.J., Gibson, G.R. and Rastall, R.A., 2002. Effect of pH and dose on the growth of gut bacteria on prebiotic carbohydrates in vitro. Anaerobe 8: 287-292.
'Effect of pH and dose on the growth of gut bacteria on prebiotic carbohydrates in vitro ' () 8 Anaerobe : 287 -292.
-
Payne, A.N., Chassard, C., Zimmermann, M., Muller, P., Stinca, S. and Lacroix, C., 2011. The metabolic activity of gut microbiota in obese children is increased compared with normal-weight children and exhibits more exhaustive substrate utilization. Nutrition and Diabetes 1: e12.
'The metabolic activity of gut microbiota in obese children is increased compared with normal-weight children and exhibits more exhaustive substrate utilization ' () 1 Nutrition and Diabetes : e12.
-
Payne, A.N., Zihler, A., Chassard, C. and Lacroix, C., 2012. Advances and perspectives in in vitro human gut fermentation modeling. Trends in Biotechnology 30: 17-25.
'Advances and perspectives in in vitro human gut fermentation modeling ' () 30 Trends in Biotechnology : 17 -25.
-
Pham, V.T., Lacroix, C., Braegger, C.P. and Chassard, C., 2017. Lactate-utilizing community is associated with gut microbiota dysbiosis in colicky infants. Scientific Reports 7: 11176.
'Lactate-utilizing community is associated with gut microbiota dysbiosis in colicky infants ' () 7 Scientific Reports : 11176.
-
Possemiers, S., Pinheiro, I., Verhelst, A., Van den Abbeele, P., Maignien, L., Laukens, D., Reeves, S.G., Robinson, L.E., Raas, T., Schneider, Y.J., Van de Wiele, T. and Marzorati, M., 2013. A dried yeast fermentate selectively modulates both the luminal and mucosal gut microbiota and protects against inflammation, as studied in an integrated in vitro approach. Journal of Agricultural and Food Chemistry 61: 9380-9392.
'A dried yeast fermentate selectively modulates both the luminal and mucosal gut microbiota and protects against inflammation, as studied in an integrated in vitro approach ' () 61 Journal of Agricultural and Food Chemistry : 9380 -9392.
-
Probert, H.M. and Gibson, G.R., 2002. Investigating the prebiotic and gas-generating effects of selected carbohydrates on the human colonic microflora. Letters in Applied Microbiology 35: 473-480.
'Investigating the prebiotic and gas-generating effects of selected carbohydrates on the human colonic microflora ' () 35 Letters in Applied Microbiology : 473 -480.
-
Rajilic-Stojanovic, M., Heilig, H.G., Tims, S., Zoetendal, E.G. and De Vos, W.M., 2012. Long-term monitoring of the human intestinal microbiota composition. Environmental Microbiology 15: 1146-1159.
'Long-term monitoring of the human intestinal microbiota composition ' () 15 Environmental Microbiology : 1146 -1159.
-
Rehman, A., Heinsen, F.A., Koenen, M.E., Venema, K., Knecht, H., Hellmig, S., Schreiber, S. and Ott, S.J., 2012. Effects of probiotics and antibiotics on the intestinal homeostasis in a computer controlled model of the large intestine. BMC Microbiology 12: 47.
'Effects of probiotics and antibiotics on the intestinal homeostasis in a computer controlled model of the large intestine ' () 12 BMC Microbiology : 47.
-
Reimer, R.A., Maathuis, A.J., Venema, K., Lyon, M.R., Gahler, R.J. and Wood, S., 2014. Effect of the novel polysaccharide PolyGlycopleX(R) on short-chain fatty acid production in a computer-controlled in vitro model of the human large intestine. Nutrients 6: 1115-1127.
'Effect of the novel polysaccharide PolyGlycopleX(R) on short-chain fatty acid production in a computer-controlled in vitro model of the human large intestine ' () 6 Nutrients : 1115 -1127.
-
Robinson, C.D., Auchtung, J.M., Collins, J. and Britton, R.A., 2014. Epidemic Clostridium difficile strains demonstrate increased competitive fitness compared to nonepidemic isolates. Infection and Immunity 82: 2815-2825.
'Epidemic Clostridium difficile strains demonstrate increased competitive fitness compared to nonepidemic isolates ' () 82 Infection and Immunity : 2815 -2825.
-
Rodriguez-Colinas, B., Kolida, S., Baran, M., Ballesteros, A.O., Rastall, R.A. and Plou, F.J., 2013. Analysis of fermentation selectivity of purified galacto-oligosaccharides by in vitro human faecal fermentation. Applied Microbiology and Biotechnology 97: 5743-5752.
'Analysis of fermentation selectivity of purified galacto-oligosaccharides by in vitro human faecal fermentation ' () 97 Applied Microbiology and Biotechnology : 5743 -5752.
-
Rycroft, C.E., Jones, M.R., Gibson, G.R. and Rastall, R.A., 2001a. A comparative in vitro evaluation of the fermentation properties of prebiotic oligosaccharides. Journal of Applied Microbiology 91: 878-887.
'A comparative in vitro evaluation of the fermentation properties of prebiotic oligosaccharides ' () 91 Journal of Applied Microbiology : 878 -887.
-
Rycroft, C.E., Jones, M.R., Gibson, G.R. and Rastall, R.A., 2001b. Fermentation properties of gentio-oligosaccharides. Letters in Applied Microbiology 32: 156-161.
'Fermentation properties of gentio-oligosaccharides ' () 32 Letters in Applied Microbiology : 156 -161.
-
Sadaghian Sadabad, M., Von Martels, J.Z., Khan, M.T., Blokzijl, T., Paglia, G., Dijkstra, G., Harmsen, H.J. and Faber, K.N., 2015. A simple coculture system shows mutualism between anaerobic faecalibacteria and epithelial Caco-2 cells. Scientific Reports 5: 17906.
'A simple coculture system shows mutualism between anaerobic faecalibacteria and epithelial Caco-2 cells ' () 5 Scientific Reports : 17906.
-
Salazar, N., Ruas-Madiedo, P., Kolida, S., Collins, M., Rastall, R., Gibson, G. and De Los Reyes-Gavilan, C.G., 2009. Exopolysaccharides produced by Bifidobacterium longum IPLA E44 and Bifidobacterium animalis subsp. lactis IPLA R1 modify the composition and metabolic activity of human faecal microbiota in pH-controlled batch cultures. International Journal of Food Microbiology 135: 260-267.
'Exopolysaccharides produced by Bifidobacterium longum IPLA E44 and Bifidobacterium animalis subsp ' () 135 International Journal of Food Microbiology : 260 -267.
-
Sanz, M.L., Polemis, N., Morales, V., Corzo, N., Drakoularakou, A., Gibson, G.R. and Rastall, R.A., 2005. In vitro investigation into the potential prebiotic activity of honey oligosaccharides. Journal of Agricultural and Food Chemistry 53: 2914-2921.
'In vitro investigation into the potential prebiotic activity of honey oligosaccharides ' () 53 Journal of Agricultural and Food Chemistry : 2914 -2921.
-
Sarbini, S.R., Kolida, S., Deaville, E.R., Gibson, G.R. and Rastall, R.A., 2014. Potential of novel dextran oligosaccharides as prebiotics for obesity management through in vitro experimentation. British Journal of Nutrition 112: 1303-1314.
'Potential of novel dextran oligosaccharides as prebiotics for obesity management through in vitro experimentation ' () 112 British Journal of Nutrition : 1303 -1314.
-
Sarbini, S.R., Kolida, S., Naeye, T., Einerhand, A., Brison, Y., Remaud-Simeon, M., Monsan, P., Gibson, G.R. and Rastall, R.A., 2011. In vitro fermentation of linear and alpha-1,2-branched dextrans by the human fecal microbiota. Applied and Environmental Microbiology 77: 5307-5315.
'In vitro fermentation of linear and alpha-1,2-branched dextrans by the human fecal microbiota ' () 77 Applied and Environmental Microbiology : 5307 -5315.
-
Saulnier, D.M., Gibson, G.R. and Kolida, S., 2008. In vitro effects of selected synbiotics on the human faecal microbiota composition. FEMS Microbiology Ecology 66: 516-527.
'In vitro effects of selected synbiotics on the human faecal microbiota composition ' () 66 FEMS Microbiology Ecology : 516 -527.
-
Sekirov, I., Russell, S.L., Antunes, L.C. and Finlay, B.B., 2010. Gut microbiota in health and disease. Physiological Reviews 90: 859-904.
'Gut microbiota in health and disease ' () 90 Physiological Reviews : 859 -904.
-
Sender, R., Fuchs, S. and Milo, R., 2016. Revised estimates for the number of human and bacteria cells in the body. PLoS Biology 14: e1002533.
'Revised estimates for the number of human and bacteria cells in the body ' () 14 PLoS Biology : e1002533.
-
Shah, P., Fritz, J.V., Glaab, E., Desai, M.S., Greenhalgh, K., Frachet, A., Niegowska, M., Estes, M., Jager, C., Seguin-Devaux, C., Zenhausern, F. and Wilmes, P., 2016. A microfluidics-based in vitro model of the gastrointestinal human-microbe interface. Nature Communications 7: 11535.
'A microfluidics-based in vitro model of the gastrointestinal human-microbe interface ' () 7 Nature Communications : 11535.
-
Terpend, K., Possemiers, S., Daguet, D. and Marzorati, M., 2013. Arabinogalactan and fructo-oligosaccharides have a different fermentation profile in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME (R)). Environmental Microbiology Reports 5: 595-603.
'Arabinogalactan and fructo-oligosaccharides have a different fermentation profile in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME (R)) ' () 5 Environmental Microbiology Reports : 595 -603.
-
Tottey, W., Feria-Gervasio, D., Gaci, N., Laillet, B., Pujos, E., Martin, J.F., Sebedio, J.L., Sion, B., Jarrige, J.F., Alric, M. and Brugere, J.F., 2016. Colonic transit time is a driven force of the gut microbiota composition and metabolism: in vitro evidence. Journal of Neurogastroenterology and Motility 23: 124-134.
'Colonic transit time is a driven force of the gut microbiota composition and metabolism: in vitro evidence ' () 23 Journal of Neurogastroenterology and Motility : 124 -134.
-
Turroni, F., Ribbera, A., Foroni, E., Van Sinderen, D. and Ventura, M., 2008. Human gut microbiota and bifidobacteria: from composition to functionality. Antonie Van Leeuwenhoek 94: 35-50.
'Human gut microbiota and bifidobacteria: from composition to functionality ' () 94 Antonie Van Leeuwenhoek : 35 -50.
-
Tzortzis, G., Goulas, A.K., Baillon, M.L., Gibson, G.R. and Rastall, R.A., 2004. In vitro evaluation of the fermentation properties of galactooligosaccharides synthesised by alpha-galactosidase from Lactobacillus reuteri. Applied Microbiology and Biotechnology 64: 106-111.
'In vitro evaluation of the fermentation properties of galactooligosaccharides synthesised by alpha-galactosidase from Lactobacillus reuteri ' () 64 Applied Microbiology and Biotechnology : 106 -111.
-
Ulluwishewa, D., Anderson, R.C., Young, W., McNabb, W.C., Van Baarlen, P., Moughan, P.J., Wells, J.M. and Roy, N.C., 2015. Live Faecalibacterium prausnitzii in an apical anaerobic model of the intestinal epithelial barrier. Cellular Microbiology 17: 226-240.
'Live Faecalibacterium prausnitzii in an apical anaerobic model of the intestinal epithelial barrier ' () 17 Cellular Microbiology : 226 -240.
-
Van de Wiele, T., Van den Abbeele, P., Ossieur, W., Possemiers, S. and Marzorati, M., 2015. The Simulator of the Human Intestinal Microbial Ecosystem (SHIME®). In: Verhoeckx, K., Cotter, P., López-Expósito, I., Kleiveland, C., Lea, T., Mackie, A., Requena, T., Swiatecka, D. and Wichers, H. (eds.) The impact of food bio-actives on gut health. Springer, New York, NY, USA, pp. 305-317.
'The Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) ', in The impact of food bio-actives on gut health , () 305 -317.
-
Van den Abbeele, P., Belzer, C., Goossens, M., Kleerebezem, M., De Vos, W.M., Thas, O., De Weirdt, R., Kerckhof, F.M. and Van de Wiele, T., 2013. Butyrate-producing Clostridium cluster XIVa species specifically colonize mucins in an in vitro gut model. ISME Journal 7: 949-961.
'Butyrate-producing Clostridium cluster XIVa species specifically colonize mucins in an in vitro gut model ' () 7 ISME Journal : 949 -961.
-
Van den Abbeele, P., Grootaert, C., Marzorati, M., Possemiers, S., Verstraete, W., Gerard, P., Rabot, S., Bruneau, A., El Aidy, S., Derrien, M., Zoetendal, E., Kleerebezem, M., Smidt, H. and Van de Wiele, T., 2010. Microbial community development in a dynamic gut model is reproducible, colon region specific, and selective for Bacteroidetes and Clostridium cluster IX. Applied and Environmental Microbiology 76: 5237-5246.
'Microbial community development in a dynamic gut model is reproducible, colon region specific, and selective for Bacteroidetes and Clostridium cluster IX ' () 76 Applied and Environmental Microbiology : 5237 -5246.
-
Van den Abbeele, P., Marzorati, M., Derde, M., De Weirdt, R., Joan, V., Possemiers, S. and Van de Wiele, T., 2016. Arabinoxylans, inulin and Lactobacillus reuteri 1063 repress the adherent-invasive Escherichia coli from mucus in a mucosa-comprising gut model. NPJ Biofilms and Microbiomes 2: 16016.
'Arabinoxylans, inulin and Lactobacillus reuteri 1063 repress the adherent-invasive Escherichia coli from mucus in a mucosa-comprising gut model ' () 2 NPJ Biofilms and Microbiomes : 16016.
-
Van den Abbeele, P., Roos, S., Eeckhaut, V., MacKenzie, D.A., Derde, M., Verstraete, W., Marzorati, M., Possemiers, S., Vanhoecke, B., Van Immerseel, F. and Van de Wiele, T., 2012. Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli. Microbial Biotechnology 5: 106-115.
'Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli ' () 5 Microbial Biotechnology : 106 -115.
-
Van Nuenen, M.H.M.C., Meyer, P.D. and Venema, K., 2003. The effect of various inulins and Clostridium difficile on the metabolic activity of the human colonic microbiota in vitro. Microbial Ecology in Health and Disease 15: 137-144.
'The effect of various inulins and Clostridium difficile on the metabolic activity of the human colonic microbiota in vitro ' () 15 Microbial Ecology in Health and Disease : 137 -144.
-
Venema, K. and Van den Abbeele, P., 2013. Experimental models of the gut microbiome. Best Practice and Research: Clinical Gastroenterology 27: 115-126.
'Experimental models of the gut microbiome ' () 27 Best Practice and Research: Clinical Gastroenterology : 115 -126.
-
Venema, K., Van Nuenen, M.H.M.C., Van den Heuvel, E.G. Pool, W. and Van der Vossen, J.M.B.M., 2003. The effect of lactulose on the composition of the intestinal microbiota and short-chain fatty acid production in human volunteers and a computer-controlled model of the proximal large intestine. Microbial Ecology in Health and Disease 15: 97-105.
'The effect of lactulose on the composition of the intestinal microbiota and short-chain fatty acid production in human volunteers and a computer-controlled model of the proximal large intestine ' () 15 Microbial Ecology in Health and Disease : 97 -105.
-
Walker, A.W., Duncan, S.H., McWilliam Leitch, E.C., Child, M.W. and Flint, H.J., 2005. pH and peptide supply can radically alter bacterial populations and short-chain fatty acid ratios within microbial communities from the human colon. Applied and Environmental Microbiology 71: 3692-3700.
'pH and peptide supply can radically alter bacterial populations and short-chain fatty acid ratios within microbial communities from the human colon ' () 71 Applied and Environmental Microbiology : 3692 -3700.
-
Wang, X. and Gibson, G.R., 1993. Effects of the in vitro fermentation of oligofructose and inulin by bacteria growing in the human large intestine. Journal of Applied Bacteriology 75: 373-380.
'Effects of the in vitro fermentation of oligofructose and inulin by bacteria growing in the human large intestine ' () 75 Journal of Applied Bacteriology : 373 -380.
-
Warner, B.B. and Tarr, P.I., 2016. Necrotizing enterocolitis and preterm infant gut bacteria. Seminars in Fetal and Neonatal Medicine 21: 394-399.
'Necrotizing enterocolitis and preterm infant gut bacteria ' () 21 Seminars in Fetal and Neonatal Medicine : 394 -399.
-
Wichienchot, S., Prasertsan, P., Hongpattarakere, T., Gibson, G.R. and Rastall, R.A., 2006. In vitro fermentation of mixed linkage glucooligosaccharides produced by Gluconobacter oxydans NCIMB 4943 by the human colonic microflora. Current Issues in Intestinal Microbiology 7: 7-12.
'In vitro fermentation of mixed linkage glucooligosaccharides produced by Gluconobacter oxydans NCIMB 4943 by the human colonic microflora ' () 7 Current Issues in Intestinal Microbiology : 7 -12.
-
Yang, J., Keshavarzian, A. and Rose, D.J., 2013. Impact of dietary fiber fermentation from cereal grains on metabolite production by the fecal microbiota from normal weight and obese individuals. Journal of Medicinal Food 16: 862-867.
'Impact of dietary fiber fermentation from cereal grains on metabolite production by the fecal microbiota from normal weight and obese individuals ' () 16 Journal of Medicinal Food : 862 -867.
-
Zihler, A., Gagnon, M., Chassard, C., Hegland, A., Stevens, M.J., Braegger, C.P. and Lacroix, C., 2010. Unexpected consequences of administering bacteriocinogenic probiotic strains for Salmonella populations, revealed by an in vitro colonic model of the child gut. Microbiology 156: 3342-3353.
'Unexpected consequences of administering bacteriocinogenic probiotic strains for Salmonella populations, revealed by an in vitro colonic model of the child gut ' () 156 Microbiology : 3342 -3353.
-
Zihler, A., Gagnon, M., Chassard, C. and Lacroix, C., 2011. Protective effect of probiotics on Salmonella infectivity assessed with combined in vitro gut fermentation-cellular models. BMC Microbiology 11: 264.
'Protective effect of probiotics on Salmonella infectivity assessed with combined in vitro gut fermentation-cellular models ' () 11 BMC Microbiology : 264.
-
Zihler Berner, A., Fuentes, S., Dostal, A., Payne, A.N., Vazquez Gutierrez, P., Chassard, C., Grattepanche, F., De Vos, W.M. and Lacroix, C., 2013. Novel Polyfermentor intestinal model (PolyFermS) for controlled ecological studies: validation and effect of pH. PLoS ONE 8: e77772.
'Novel Polyfermentor intestinal model (PolyFermS) for controlled ecological studies: validation and effect of pH ' () 8 PLoS ONE : e77772.
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The application of in vitro human intestinal models on the screening and development of pre- and probiotics
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The importance of the gut microbiota community on host’s health and disease has long been recognised and is well documented. The development of pro- and prebiotic interventions offers an opportunity for the modulation of the gut microbiota towards long lasting health. In vitro fermentation models were developed as a powerful tool to study the impact of pro- and prebiotics on the gut microbiota under tightly controlled conditions, which allow dynamic sampling over time in reactors mimicking different colon regions. These models have been further evolved to suit specific experimental purposes, e.g. including immobilised faecal microbiota, peristaltic movement, mucin microcosm and the ability to perform treatments in parallel. In this review we discuss the advantages, disadvantages and technical considerations of the most frequently used models. We further focus on recent advances in the application of these models in prebiotics and probiotics research and outline their predictability for clinical research.
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