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An assay was developed that tested the ability of Lactobacillus acidophilus to outcompete a challenge of Escherichia coli in a mixed culture containing different test nutrients. Using this assay, addition of fructo-oligosaccharide to the media allowed L. acidophilus to outcompete a challenge of E. coli, whereas in a mixed culture without the prebiotic the trend was reversed. Growth curves generated for E. coli in a single culture showed that fructo-oligosaccharide did not affect growth, indicating that the carbohydrate was not toxic to E. coli. This indicates that fructo-oligosaccharides may increase the ability of beneficial microbes to outcompete a pathogenic challenge. These results were confirmed using a skin simulant model that incorporates growth of the organisms at an air-surface interface to mimic the vulvar environment. It is possible to use a co-culture assay as an in vitro screening tool to define nutrients that confer a competitive advantage to beneficial flora specific to the female urogenital tract.
Barrangou, R., Altermann, E., Hutkins, R., Cano, R. and Klaenhammer, T.R., 2003. Functional and comparative genomic analyses of an operon involved in fructooligosaccharide utilization by Lactobacillus acidophilus. Proceedings of the National Academy of Sciences of the USA 100: 8957-8962.
'Functional and comparative genomic analyses of an operon involved in fructooligosaccharide utilization by Lactobacillus acidophilus ' () 100 Proceedings of the National Academy of Sciences of the USA : 8957 -8962.
Fooks, L.J. and Gibson, G.R., 2002. In vitro investigations of the effect of probiotics and prebiotics on selected human intestinal pathogens. FEMS Micriobiology Ecology 39: 67-75.
'In vitro investigations of the effect of probiotics and prebiotics on selected human intestinal pathogens ' () 39 FEMS Micriobiology Ecology : 67 -75.
Giorgi, A., Torriani, S., Dellaglio, F., Bo, G., Stola, E. and Bernuzzi, L., 1987. Identification of vaginal lactobacilli from asymptomatic women. Microbiologica 10: 377-384.
'Identification of vaginal lactobacilli from asymptomatic women ' () 10 Microbiologica : 377 -384.
Gregoire, A.T., Kandil, O. and Ledger, W. J., 1971. The glycogen content of human vaginal epithelial tissue. Fertility and Sterility 22: 64-68.
'The glycogen content of human vaginal epithelial tissue ' () 22 Fertility and Sterility : 64 -68.
Kaplan, H. and Hutkins, R.W., 2003. Metabolism of fructooligosaccharides by Lactobacillus paracasei 1195. Applied and Environmental Microbiology 69: 2217-2222.
'Metabolism of fructooligosaccharides by Lactobacillus paracasei 1195 ' () 69 Applied and Environmental Microbiology : 2217 -2222.
Makras, L., Van Acker, G. and De Vuyst, L., 2005. Lactobacillus paracasei subsp. paracasei 8700: 2 degrades inulin-type fructans exhibiting different degrees of polymerization. Applied and Environmental Microbiology 71: 6531-6537.
'Lactobacillus paracasei subsp. paracasei 8700: 2 degrades inulin-type fructans exhibiting different degrees of polymerization ' () 71 Applied and Environmental Microbiology : 6531 -6537.
Naik, S., Bouladoux, N., Wilhelm, C., Molloy, M.J., Salcedo, R., Kastenmuller, W., Deming, C., Quinones, M., Koo L., Conlan, S., Spencer, S., Hall, J.A., Dzutsev, A., Kong, H., Campbell, D.J., Trinchieri, G., Segre, J.A. and Belkaid, Y., 2012. Compartmentalized control of skin immunity by resident commensals. Science 337: 1115-1119.
'Compartmentalized control of skin immunity by resident commensals ' () 337 Science : 1115 -1119.
Paavonen, J., 1983. Physiology and ecology of the vagina. Scandinavian Journal of Infectious Diseases 40 Suppl.: 31-35.
'Physiology and ecology of the vagina ' () 40 Scandinavian Journal of Infectious Diseases : 31 -35.
Reid, G., 2001. Probiotic agents to protect the urogenital tract against infection. American Journal of Clinical Nutrition 73 Suppl. 2: 437S-443S.
'Probiotic agents to protect the urogenital tract against infection ' () 73 American Journal of Clinical Nutrition : 437S -443S.
Reid, G. and Bruce, A.W., 2001. Could probiotics be an option for treating and preventing urogenital infections? Medscape Women’s Health 6: 9.
'Could probiotics be an option for treating and preventing urogenital infections? ' () 6 Medscape Women’s Health : 9.
Roberfroid, M., Gibson, G.R., Hoyles, L., McCartney, A.L., Rastall, R., Rowland, I., Wolvers, D., Watzl, B., Szajewska, H., Stahl, B., Guarner, F., Respondek, F., Whelan, K., Coxam, V., Davicco, M.-J., Léotoing, L., Wittrant, Y., Delzenne, N.M., Cani, P.D., Neyrinck, A.M. and Meheust, A., 2010. Prebiotic effects: metabolic and health benefits. British Journal of Nutrition 104 Suppl. E-S1: S1-S63.
'Prebiotic effects: metabolic and health benefits ' () 104 British Journal of Nutrition : S1 -S63.
Rousseau, V., Lepargneur, J.P., Roques, C., Remaud-Simeon, M. and Paul, F., 2005. Prebiotic effects of oligosaccharides on selected vaginal lactobacilli and pathogenic microorganisms. Anaerobe 11: 145-153.
'Prebiotic effects of oligosaccharides on selected vaginal lactobacilli and pathogenic microorganisms ' () 11 Anaerobe : 145 -153.
Warren, R., Bauer, A., Greif, C., Wigger-Alberti, W., Jones, M.B., Roddy, M.T., Seymour, J L., Hansmann, M.A. and Elsner, P., 2005. Transepidermal water loss dynamics of human vulvar and thigh skin. Skin Pharmacology and Physiology 18: 139-143.
'Transepidermal water loss dynamics of human vulvar and thigh skin ' () 18 Skin Pharmacology and Physiology : 139 -143.
Zhou, X., Bent, S., Schneider, M., Davis, C.C., Islam, M.R. and Forney, L.J., 2004. Characterization of vaginal microbial communities in adult healthy women using cultivation-independent methods. Microbiology 150: 2565-2573.
'Characterization of vaginal microbial communities in adult healthy women using cultivation-independent methods ' () 150 Microbiology : 2565 -2573.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
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| PDF-Downloads | 355 | 111 | 3 |
An assay was developed that tested the ability of Lactobacillus acidophilus to outcompete a challenge of Escherichia coli in a mixed culture containing different test nutrients. Using this assay, addition of fructo-oligosaccharide to the media allowed L. acidophilus to outcompete a challenge of E. coli, whereas in a mixed culture without the prebiotic the trend was reversed. Growth curves generated for E. coli in a single culture showed that fructo-oligosaccharide did not affect growth, indicating that the carbohydrate was not toxic to E. coli. This indicates that fructo-oligosaccharides may increase the ability of beneficial microbes to outcompete a pathogenic challenge. These results were confirmed using a skin simulant model that incorporates growth of the organisms at an air-surface interface to mimic the vulvar environment. It is possible to use a co-culture assay as an in vitro screening tool to define nutrients that confer a competitive advantage to beneficial flora specific to the female urogenital tract.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 0 | 0 | 0 |
| Gesamttextansichten | 346 | 123 | 20 |
| PDF-Downloads | 355 | 111 | 3 |