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Transcriptomic response of immune signalling pathways in intestinal epithelial cells exposed to lipopolysaccharides, Gram-negative bacteria or potentially probiotic microbes
in Beneficial Microbes2Agropur Coopérative, 4700 Rue Armand Frappier, J3Z 1G5 Saint-Hubert, QC, Canada
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ttompkins@lallemand.com
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Abstract
In order to understand the appropriate use of potentially probiotic Gram-positive microbes through their introduction in the gut microbiome, it is necessary to understand the influence of individual bacteria on the host-response system at a cellular level. In the present study, we have shown that lipopolysaccharides, flagellated Gram-negative bacteria, potentially probiotic Gram-positive bacteria and yeast interact differently with human intestinal epithelial cells with a custom-designed expression microarray evaluating 17 specific host-response pathways. Only lipopolysaccharides and flagellated Gram-negative bacteria induced inflammatory response, while a subset of Gram-positive microbes had anti-inflammatory potential. The main outcome from the study was the differential regulation of the central mitogen-activated protein kinase signalling pathway by these Gram-positive microbes versus commensal/pathogenic Gram-negative bacteria. The microarray was efficient to highlight the impact of individual bacteria on the response of intestinal epithelial cells, but quantitative real-time polymerase chain reaction validation demonstrated some underestimation for down-regulated genes by the microarray. This immune array will allow us to better understand the mechanisms underlying microbe-induced host immune responses.
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Birkenkamp-Demtroder, K., Christensen, L.L., Olesen, S.H., Frederiksen, C.M., Laiho, P., Aaltonen, L.A., Laurberg, S., Sorensen, F.B., Hagemann, R. and Orntoft, T.F., 2002. Gene expression in colorectal cancer. Cancer Research 62: 4352-4363.
'Gene expression in colorectal cancer ' () 62 Cancer Research : 4352 -4363.
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Cui, W., Taub, D.D. and Gardner, K., 2007. qPrimerDepot: a primer database for quantitative real time PCR. Nucleic Acids Research 35: D805-D809.
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Dydensborg, A.B., Herring, E., Auclair, J., Tremblay, E. and Beaulieu, J.F., 2006. Normalizing genes for quantitative RT-PCR in differentiating human intestinal epithelial cells and adenocarcinomas of the colon. American Journal of Physiology - Gastrointestinal and Liver Physiology 290: G1067-G1074.
'Normalizing genes for quantitative RT-PCR in differentiating human intestinal epithelial cells and adenocarcinomas of the colon ' () 290 American Journal of Physiology - Gastrointestinal and Liver Physiology : G1067 -G1074.
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Locati, M., Deuschle, U., Massardi, M.L., Martinez, F.O., Sironi, M., Sozzani, S., Bartfai, T. and Mantovani, A., 2002. Analysis of the gene expression profile activated by the CC chemokine ligand 5/ RANTES and by lipopolysaccharide in human monocytes. Journal of Immunology 168: 3557-3562.
'Analysis of the gene expression profile activated by the CC chemokine ligand 5/ RANTES and by lipopolysaccharide in human monocytes ' () 168 Journal of Immunology : 3557 -3562.
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Mookherjee, N., Brown, K.L., Bowdish, D.M., Doria, S., Falsafi, R., Hokamp, K., Roche, F.M., Mu, R., Doho, G.H., Pistolic, J., Powers, J.P., Bryan, J., Brinkman, F.S. and Hancock, R.E., 2006. Modulation of the TLR-mediated inflammatory response by the endogenous human host defense peptide LL-37. Journal of Immunology 176: 2455-2464.
'Modulation of the TLR-mediated inflammatory response by the endogenous human host defense peptide LL-37 ' () 176 Journal of Immunology : 2455 -2464.
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Nagao, M., Nakajima, Y., Kanehiro, H., Hisanaga, M., Aomatsu, Y., Ko, S., Tatekawa, Y., Ikeda, N., Kanokogi, H., Urizono, Y., Kobayashi, T., Shibaji, T., Kanamura, T., Ogawa, S. and Nakano, H., 2000. The impact of interferon gamma receptor expression on the mechanism of escape from host immune surveillance in hepatocellular carcinoma. Hepatology 32: 491-500.
'The impact of interferon gamma receptor expression on the mechanism of escape from host immune surveillance in hepatocellular carcinoma ' () 32 Hepatology : 491 -500.
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Reichelt, W.H., Yndestad, A., Wright, M.S., Elgjo, K., Haug, T. and Reichelt, K.L., 2003. The colon mitosis-inhibitor pyroglutamyl-histidyl-glycine alters expression of immediate-early cancer-related genes in HT-29 cells. Anticancer Research 23: 1229-1234.
'The colon mitosis-inhibitor pyroglutamyl-histidyl-glycine alters expression of immediate-early cancer-related genes in HT-29 cells ' () 23 Anticancer Research : 1229 -1234.
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Uray, I.P., Connelly, J.H., Thomazy, V., Shipley, G.L., Vaughn, W.K., Frazier, O.H., Taegtmeyer, H. and Davies, P.J., 2002. Left ventricular unloading alters receptor tyrosine kinase expression in the failing human heart. Journal of Heart and Lung Transplantation 21: 771-782.
'Left ventricular unloading alters receptor tyrosine kinase expression in the failing human heart ' () 21 Journal of Heart and Lung Transplantation : 771 -782.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 14 | 0 | 0 |
| Gesamttextansichten | 377 | 155 | 28 |
| PDF-Downloads | 378 | 159 | 11 |
Transcriptomic response of immune signalling pathways in intestinal epithelial cells exposed to lipopolysaccharides, Gram-negative bacteria or potentially probiotic microbes
in Beneficial Microbes2Agropur Coopérative, 4700 Rue Armand Frappier, J3Z 1G5 Saint-Hubert, QC, Canada
Search for other papers by J. Audy in
Current site
Google Scholar
PubMed
Search for other papers by O. Mathieu in
Current site
Google Scholar
PubMed
Search for other papers by J. Belvis in
Current site
Google Scholar
PubMed
ttompkins@lallemand.com
Search for other papers by T.A. Tompkins in
Current site
Google Scholar
PubMed
Abstract
In order to understand the appropriate use of potentially probiotic Gram-positive microbes through their introduction in the gut microbiome, it is necessary to understand the influence of individual bacteria on the host-response system at a cellular level. In the present study, we have shown that lipopolysaccharides, flagellated Gram-negative bacteria, potentially probiotic Gram-positive bacteria and yeast interact differently with human intestinal epithelial cells with a custom-designed expression microarray evaluating 17 specific host-response pathways. Only lipopolysaccharides and flagellated Gram-negative bacteria induced inflammatory response, while a subset of Gram-positive microbes had anti-inflammatory potential. The main outcome from the study was the differential regulation of the central mitogen-activated protein kinase signalling pathway by these Gram-positive microbes versus commensal/pathogenic Gram-negative bacteria. The microarray was efficient to highlight the impact of individual bacteria on the response of intestinal epithelial cells, but quantitative real-time polymerase chain reaction validation demonstrated some underestimation for down-regulated genes by the microarray. This immune array will allow us to better understand the mechanisms underlying microbe-induced host immune responses.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 14 | 0 | 0 |
| Gesamttextansichten | 377 | 155 | 28 |
| PDF-Downloads | 378 | 159 | 11 |
