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Lactobacillus crispatus DSM25988 as novel bioactive agent to co-aggregate Streptococcus pyogenes and to exclude it by binding to human cells
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Streptococcus pyogenes, a group A streptococcus, is the major bacterial pathogen responsible for acute bacterial infection of the human oropharynx and the causative agent of scarlet fever. Estimates of the global burden of S. pyogenes related diseases revealed 616 million cases of pharyngitis, and at least 517,000 deaths due to severe invasive diseases and sequelae. Here we describe Lactobacillus crispatus DSM25988 that was identified among hundreds of Lactobacillus strains (referring to all organisms that were classified as Lactobacillaceae until 2020) showing ability to prevent adhesion of S. pyogenes to Detroit 562 cells, and to exhibit a masking and co-aggregating effect on S. pyogenes in vitro. L. crispatus DSM25988 also inhibits invasion of cultured human epithelial pharyngeal cells by S. pyogenes. Competitive binding to fibronectin might be involved in the inhibition process. Antiviral activity of the L. crispatus DSM25988 cells were identified in an in vitro cell model demonstrating that L. crispatus effectively excludes viruses from epithelial cells using SARS-CoV2 proteins as a model. This finding points to the potential of DSM25988 to protect cells from virus infection. Biological activity is retained in heat treated cells. The heat-treated Lactobacillus strain was further developed into functional throat lozenges, wherein its biological activity is stably maintained in the formulation. Lozenges containing L. crispatus DSM25988 underwent testing in an uncontrolled, prospective user study in 44 subjects with symptoms of sore throat for a period of up to 14 days. The study data shows promising safety and efficacy of the medical device when used against symptoms of sore throat like scratchy feeling, hoarse voice and swallowing pain.
-
Abramov, V., Khlebnikov, V., Kosarev, I., Bairamova, G., Vasilenko, R., Suzina, N., Machulin, A., Sakulin, V., Kulikova, N., Vasilenko, N., Karlyshev, A., Uversky, V., Chikindas, M.L. and Melnikov, V., 2014. Probiotic properties of Lactobacillus crispatus 2,029: homeostatic interaction with cervicovaginal epithelial cells and antagonistic activity to genitourinary pathogens. Probiotics and Antimicrobial Proteins 6: 165-176. https://doi.org/10.1007/s12602-014-9164-4
-
Andreoletti, O., Budka, H., Buncic, S., Colin, P., Collins, J.D., De Koeijer, A., Griffin, J., Havelaar, A., Hope, J., Klein, G., Kruse, H., Magnino, S., López, A.M., McLauchlin, J., Nguyen-Thé, C., Noeckler, K., Noerrung, B., Prieto Maradona, M., Roberts, T., Vågsholm, I. and Vanopdenbosch, E., 2008. Scientific Opinion of the Panel on Biological Hazards on a request from EFSA on the maintenance of the QPS list of microorganisms intentionally added to food or feed. EFSA Journal 6: 923. https://doi.org/10.2903/j.efsa.2008.923
-
Antikainen, J., Anton, L., Sillanpää, J. and Korhonen, T.K., 2002. Domains in the S-layer protein CbsA of Lactobacillus crispatus involved in adherence to collagens, laminin and lipoteichoic acids and in self-assembly. Molecular Microbiology 46: 381-394. https://doi.org/10.1046/j.1365-2958.2002.03180.x
-
Beganović, J., Kos, B., Leboš Pavunc, A., Uroić, K., Jokić, M. and Šušković, J., 2014. Traditionally produced sauerkraut as source of autochthonous functional starter cultures. Microbiological Research 169: 623-632. https://doi.org/10.1016/j.micres.2013.09.015
-
Buckley, M., Lacey, S., Doolan, A., Goodbody, E. and Seamans, K., 2018. The effect of Lactobacillus reuteri supplementation in Helicobacter pylori infection: a placebo-controlled, single-blind study. BMC Nutrition 4: 48. https://doi.org/10.1186/s40795-018-0257-4
-
Carapetis, J.R., Steer, A.C., Mulholland, E.K. and Weber, M., 2005. The global burden of group A streptococcal diseases. The Lancet Infectious Diseases 5: 685-694. https://doi.org/10.1016/S1473-3099(05)70267-X
-
Carmo, M.S., Noronha, F.M.F., Arruda, M.O., Costa, Ê.P.S., Bomfim, M.R.Q., Monteiro, A.S., Ferro, T.A.F., Fernandes, E.S., Girón, J.A. and Monteiro-Neto, V., 2016. Lactobacillus fermentum ATCC 23271 displays in vitro inhibitory activities against Candida spp. Frontiers in Microbiology 7: 1722. https://doi.org/10.3389/fmicb.2016.01722
-
Ceri, H., Olson, M.E., Stremick, C., Read, R.R., Morck, D. and Buret, A., 1999. The Calgary Biofilm Device: new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms. Journal of Clinical Microbiology 37: 1771-1776. https://doi.org/10.1128/JCM.37.6.1771-1776.1999
-
Chen, X., Xu, J., Shuai, J, Chen, J, Zhang, Z. and Fang, W., 2007. The S-layer proteins of Lactobacillus crispatus strain ZJ001 is responsible for competitive exclusion against Escherichia coli O157:H7 and Salmonella Typhimurium. International Journal of Food Microbiology 115: 307-312. https://doi.org/10.1016/j.ijfoodmicro.2006.11.007
-
Courtney, H.S., Ofek, I., Simpson, W.A., Hasty, D.L., Beachey, E.H., 1986. Binding of Streptococcus pyogenes to soluble and insoluble fibronectin. Infection and Immunity 53: 454-459. https://doi.org/10.1128/iai.53.3.454-459.1986
-
Danzmann, L., Gastmeier, P., Schwab, F., and Vonberg, R.P., 2013. Health care workers causing large nosocomial outbreaks: a systematic review. BMC Infectious Diseases 13: 98. https://doi.org/10.1186/1471-2334-13-98
-
De Man, J.C., Rogosa, M. and Sharpe, M.E., 1960. A medium for the cultivation of lactobacilli. Journal of Applied Bacteriology 23: 130-135. https://doi.org/10.1111/j.1365-2672.1960.tb00188.x
-
Doron, S. and Snydman, D.R., 2015. Risk and safety of probiotics. Clinical Infectious Diseases 60: 129-134. https://doi.org/10.1093/cid/civ085
-
Ellison, A.J., Dempwolff, F., Kearns, D.B. and Raines, R.T., 2020. Role for cell-surface collagen of Streptococcus pyogenes in infections. ACS Infectious Diseases 10: 1836-1843. https://doi.org/10.1021/acsinfecdis.0c00073
-
Ellner, P.D., Stoessel, C.J., Drakeford, E. and Vasi, F., 1966. A new culture medium for medical bacteriology. American Journal of Clinical Pathology 45: 502-504. https://doi.org/10.1093/ajcp/45.4_ts.502
-
European Commission (EC), 2016. Regulation (EU) 2016/679 of the European Parliament and of the Council of 27 April 2016 on the protection of natural persons with regard to the processing of personal data and on the free movement of such data, and repealing Directive 95/46/EC (General Data Protection Regulation). Official Journal of the European Union L 119: 1-88.
-
Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO), 2002. Guidelines for the evaluation of probiotics in food. Report of a Joint FAO/WHO working group on drafting guidelines for the evaluation of probiotics in food. Available at: http://tinyurl.com/zdbrkeg.
-
Geraldo, B.M.C., Batalha, M.N., Milhan, N.V.M., Rossoni, R.D., Scorzoni, L. and Anbinder, AL., 2020. Heat-killed Lactobacillus reuteri and cell-free culture supernatant have similar effects to viable probiotics during interaction with Porphyromonas gingivalis. Journal of Periodontal Research 55: 215-220. https://doi.org/10.1111/jre.12704
-
Goh, Y.J. and Klaenhammer, T.R., 2010. Functional roles of aggregation-promoting-like factor in stress tolerance and adherence of Lactobacillus acidophilus NCFM. Applied and Environmental Microbiology 76: 5005-5012. https://doi.org/10.1128/AEM.00030-10
-
Greco, G., Pal, S., Pasqualini R. and Schnapp, L.M., 2002. Matrix fibronectin increases HIV stability and infectivity. Journal of Immunology 168: 5722-5729. https://doi.org/10.4049/jimmunol.168.11.5722
-
Harrison, J.J., Stremick, C.A., Turner, R.J., Allan, N.D., Olson, M.E. and Ceri H., 2010. Microtiter susceptibility testing of microbes growing on peg lids: a miniaturized biofilm model for high-throughput screening. Nature Protocols 5: 1236-1254. https://doi.org/10.1038/nprot.2010.71
-
He, Y., Niu, X., Wang, B., Na, R., Xiao, B. and Yang, H., 2020. Evaluation of the inhibitory effects of Lactobacillus gasseri and Lactobacillus crispatus on the adhesion of seven common lower genital tract infection-causing pathogens to vaginal epithelial cells. Frontiers in Medicine 7: 284. https://doi.org/10.3389/fmed.2020.00284
-
Henri-Dubernet, S., Desmasures, N. and Guéguen, M., 2008. Diversity and dynamics of lactobacilli populations during ripening of RDO Camembert cheese. Canadian Journal of Microbiology 54: 218-228. https://doi.org/10.1139/W07-137
-
Holz, C., Alexander, C., Balcke, C., Moré, M., Auinger, A., Bauer, M., Junker, L., Grünwald, J., Lang, C. and Pompejus, M., 2013. Lactobacillus paracasei DSMZ16671 reduces mutans streptococci: a short-term pilot study. Probiotics and Antimicrobial Proteins 5: 259-263. https://doi.org/10.1007/s12602-013-9148-9
-
Holz, C., Busjahn, A., Mehling, H., Arya, S., Boettner, M., Habibi, H. and Lang, C., 2015. Significant reduction in Helicobacter pylori load in humans with non-viable Lactobacillus reuteri DSM17648: a pilot study. Probiotics and Antimicrobial Proteins 7: 91-100. https://doi.org/10.1007/s12602-014-9181-3
-
Horie, M., Ishiyama, A., Fujihira-Ueki, Y., Sillanpää, J., Korhonen, T.K. and Toba, T., 2002. Inhibition of the adherence of Escherichia coli strains to basement membrane by Lactobacillus crispatus expressing an S-layer. Journal of Applied Microbiology 92: 396-403. https://doi.org/10.1046/j.1365-2672.2002.01539.x
-
Humphreys, G. and McBain, A., 2019. Antagonistic effects of Streptococcus and Lactobacillus probiotics in pharyngeal biofilms. Letters in Applied Microbiology 68: 303-312. https://doi.org/10.1111/lam.13133
-
Jeong, M., Kim, J.H., Lee, J.S., Kang, S.D., Shim, S., Jung, M.Y., Yang, H., Byun, S. and Lee, K.W., 2020. Heat-killed Lactobacillus brevis enhances phagocytic activity and generates immune-stimulatory effects through activating the TAK1 pathway. Journal of Microbiology and Biotechnology 30: 1395-1403. https://doi.org/10.4014/jmb.2002.02004
-
Jia, H., Ren, S. and Wang, X., 2019. Heat-killed probiotic regulates the body’s regulatory immunity to attenuate subsequent experimental autoimmune arthritis. Immunology Letters 216: 89-96. https://doi.org/10.1016/j.imlet.2019.10.009
-
Kacániová, M., Hleba, L., Pochop, J., Kádasi-Horáková, M., Fikselová, M. and Rovná, K., 2012. Determination of wine microbiota using classical method, polymerase chain method and step one realtime PCR during fermentation process. Journal of Environmental Science and Health Part B 47: 571-578. https://doi.org/10.1080/03601234.2012.665750
-
Kim, S.Y., Choi, D.J., and Chung J., 2015. Antibiotic treatment for Helicobacter pylori: is the end coming? World Journal of Gastrointestinal Pharmacology and Therapeutics 6: 183-198. https://doi.org/10.4292/wjgpt.v6.i4.183
-
Kojic, M., Jovcic, B., Strahinic, I., Begovic, J., Lozo, J., Veljovic, K. and Topisirovic, L., 2011. Cloning and expression of a novel lactococcal aggregation factor from Lactococcus lactis subsp. lactis BGKP1. BMC Microbiology 11: 265. https://doi.org/10.1186/1471-2180-11-265
-
Lang, C., Böttner, M., Holz, C., Veen, M., Ryser, M., Reindl, A., Pompejus, M. and Tanzer, J.M., 2010. Specific Lactobacillus/mutans Streptococcus co-aggregation. Journal of Dental Research 89: 175-179. https://doi.org/10.1177/0022034509356246
-
Langdon, A., Crook, N. and Dantas, G., 2016. The effects of antibiotics on the microbiome throughout development and alternative approaches for therapeutic modulation. Genome Medicine 8: 39. https://doi.org/10.1186/s13073-016-0294-z
-
Leung, H.S.Y., Li, O.T.W., Chan, R.W.Y., Chan, M.C.W., Nicholls, J.M. and Poon, L.L.M., 2012 Entry of influenza A virus with a a2,6-linked sialic acid binding preference requires host fibronectin. Journal of Virology 86: 10704-10713. https://doi.org/10.1128/JVI.01166-12
-
Lindbaek, M., Høiby, E.A., Lermark, G., Steinsholt, I.M. and Hjortdahl, P., 2004. Predictors for spread of clinical group A streptococcal tonsillitis within the household. Scandinavian Journal of Primary Health Care 22: 239-243. https://doi.org/10.1080/02813430410006729
-
Manna, S., Chowdhury, T., Chakraborty, R. and Mandal, S.M., 2020. Probiotics-derived peptides and their immunomodulatory molecules can play a preventive role against viral diseases including COVID-19. Probiotics and Antimicrobial Proteins 23: 1-13. https://doi.org/10.1007/s12602-020-09727-7
-
Melo Pereira, G., Oliveira Coelho, B., Magalhaes, A.I., Thomaz-Socol, V., and Sokol, C.R., 2018. How to select a probiotic? A review and update of methods and criteria. Biotechnology Advances 36: 2060-2076. https://doi.org/10.1016/j.biotechadv.2018.09.003
-
Meroth, C.B., Walter, J., Hertel, C., Brandt, M.J., and Hammes, W.P., 2003. Monitoring the bacterial population dynamics in sourdough fermentation processes by using PCR-denaturing gradient gel electrophoresis. Applied and Environmental Microbiology 69: 475-482. https://doi.org/10.1128/AEM.69.1475-482.2003
-
Miljkovic M., Strahinic I., Tolinacki M., Zivkovic M., Kojic S., Golic N., and Kojic M., 2015. AggLb is the largest cell-aggregation factor from Lactobacillus paracasei subsp. paracasei BGNJ1-64, functions in collagen adhesion, and pathogen exclusion in vitro. PLoS ONE 10: e0126387. https://doi.org/10.1371/journal.pone.0126387
-
Mousavi, E., Makvandi, M., Teimoori, A., Ataei, A., Ghafari, S. and Samarbaf-Zadeh, A., 2018. Antiviral effects of Lactobacillus crispatus against HSV-2 in mammalian cell lines. Journal of the Chinese Medical Association 81: 262-267. https://doi.org/10.1016/j.jcma.2017.07.010
-
Muscariello, L., De Siena, B. and Marasco, R., 2020. Lactobacillus cell surface proteins involved in interaction with mucus and extracellular matrix components. Current Microbiology 77: 3831-3841. https://doi.org/10.1007/s00284-020-02243-5
-
Ngugi, B.M., Hemmerling, A., Bukusi, E.A., Kikuvi, G., Gikunju, J., Shiboski, S., Fredricks, D.N. and Cohen, C.R., 2011. Effects of BV-associated bacteria and sexual intercourse on vaginal colonization with the probiotic Lactobacillus crispatus CTV-05. Sexually Transmitted Diseases 38: 1020-1027. https://doi.org/10.1097/OLQ.0b013e3182267ac4
-
Ojala, T., Kankainen, M., Castro, J., Cerca, N., Edelman, S., Westerlund-Wikström, B., Paulin, L., Holm, L. and Auvinen, P., 2014. Comparative genomics of Lactobacillus crispatus suggests novel mechanisms for the competitive exclusion of Gardnerella vaginalis. BMC Genomics 15: 1070. https://doi.org/10.1186/1471-2164-15-1070
-
Parolin, C., Frisco, G., Foschi, C., Giordani, B., Salvo, M., Vitali, B., Marangoni, A. and Calonghi, N., 2018. Lactobacillus crispatus BC5 interferes with Chlamydia trachomatis infectivity through integrin modulation in cervical cells. Frontiers in Microbiology 9: 1-9. https://doi.org/10.3389/fmicb.2018.02630
-
Pasolli, E., De Filippis, F., Mauriello, I.E., Cumbo, F., Walsh, A.M., Leech, J., Cotter, P.D., Segata, N. and Ercolini, D., 2020. Large-scale genome-wide analysis links lactic acid bacteria from food with the gut microbiome. Nature Communications 11: 2610. https://doi.org/10.1038/s41467-020-16438-8
-
Plant, L., Sundqvist, J., Zughaier, S., Lövkvist, L., Stephens, D.S. and Jonsson, A., 2006. Lipooligosaccharide structure contributes to multiple steps in the virulence of Neisseria meningitidis. Infection and Immunity 74: 1360-1367. https://doi.org/10.1128/IAI.74.2.1360-1367.2006
-
Reid, G., and Friendship, R., 2002. Alternatives to antibiotic use: probiotics for the gut. Animal Biotechnology 13: 97-112. https://doi.org/10.1081/abio-120005773
-
Rhoads, A. and Au, K.F., 2015. PacBio sequencing and its application. Genomics, Proteomics and Bioinformatics 13: 278-289. https://doi.org/10.1016/j.gpb.2015.08.002
-
Schachtsiek, M., Hammes, W.P. and Hertel, C., 2004. Characterization of Lactobacillus cornyformis DSM 20001T surface protein Cpf mediating coaggregation with and aggregation among pathogens. Applied and Environmental Microbiology 70: 7078-7085. https://doi.org/10.1128/AEM.70.12.7078-7085.2004
-
Sengupta, R., Altermann, E., Anderson, R.C., McNabb, W.C., Moughan, P.J. and Roy, N.C., 2013. The role of cell surface architecture of Lactobacilli in host-microbe interactions in the gastrointestinal tract. Mediators of Inflammation 2013: 237921. https://doi.org/10.1155/2013/237921
-
Shenderov, B.A., Sinitsa, A.V., Zakharchenko, M. and Lang, C., 2020. Metabiotics. Springer Nature, Berlin, Germany.
'Metabiotics', ().
-
Shulman, S.T., Bisno, A.B., Clegg, H.W., Gerber, M.A., Kaplan, E.L., Lee, G., Martin, J.M. and Van Beneden, C., 2012. Clinical practice guideline for the diagnosis and management of group A streptococcal pharyngitis. Clinical Infectious Diseases 55: e86-e102. https://doi.org/10.1093/cid/cis629
-
Silva, D.R., Sardi, J.O., Souza Pitanguic, N., Roque, S.M., Barbosa da Silva, A.C. and Rosalen, P.L., 2020. Probiotics as an alternative antimicrobial therapy: current reality and future directions. Journal of Functional Foods 73: 104080. https://doi.org/10.1016/j.jff.2020.104080
-
Stapleton, A.E., Au-Yeung, M., Hooton, T.M., Fredricks, D.N., Roberts, P.L., Czaja, C.A., Yarova-Yarovaya, Y., Fiedler, T., Cox, M. and Stamm, W.E., 2011. Randomized, placebo-controlled phase 2 trial of a Lactobacillus crispatus probiotic given intravaginally for prevention of recurrent urinary tract infection. Clinical Infectious Diseases 52: 1212-1217. https://doi.org/10.1093/cid/cir183
-
Stavropoulou, E. and Bezirtzoglou, E., 2020. Probiotics in medicine: a long debate. Frontiers in Immunology 11: 2192. https://doi.org/10.3389/fimmu.2020.02192
-
Stepanović, S., Vuković, D., Dakić, I., Savić, B. and Švabić-Vlahović, M., 2000. A modified microtiter-plate test for quantification of staphylococcal biofilm formation. Journal of Microbiological Methods 40: 175-179. https://doi.org/10.1016/S0167-7012(00)00122-6
-
Thors, V., Morales-Aza, B., Pidwill, G., Vipond, I., Muir, P. and Finn, A., 2016. Population density profiles of nasopharyngeal carriage of 5 bacterial species in pre-school children measured using quantitative PCR offer potential insights into the dynamics of transmission. Human Vaccines and Immunotherapeutics 12: 375-382. https://doi.org/10.1080/21645515.2015.1090069
-
Van Beek, S. and Priest, F.G., 2000. Decarboxylation of substituted cinnamic acids by lactic acid bacteria isolated during malt whisky fermentation. Applied and Environmental Microbiology 66: 5322-5328. https://doi.org/10.1128/AEM.66.12.5322-5328.2000
-
Walker, M.J., Barnett, T.C., McArthur, J.D., Cole, J.N., Gillen, C.M., Henningham, A., Sriprakash, K.S., Sanderson-Smith, M.L. and Nizet, V., 2014. Disease manifestations and pathogenic mechanisms of group A Streptococcus. Clinical Microbiology Reviews 27: 264-301. https://doi.org/10.1128/CMR.00101-13
-
Walter, J., 2008. Ecological role of lactobacilli in the gastrointestinal tract: implications for fundamental and biomedical research. Applied and Environmental Microbiology 74: 4985-4996. https://doi.org/10.1128/AEM.00753-08
-
Wang, H., Ma, Y., Li, R., Chen, X., Wan, L. and Zhao, W., 2019. Associations of cervicovaginal lactobacilli with high-risk human papillomavirus infection, cervical intraepithelial neoplasia, and cancer: a systematic review and meta-analysis. Journal of Infectious Diseases 220: 1243-1254. https://doi.org/10.1093/infdis/jiz325
-
You, I. and Kim, E.B., 2020. Genome-based species-specific primers for rapid identification of six species of Lactobacillus acidophilus group using multiplex PCR. PLoS ONE 15: e0230550. https://doi.org/10.1371/journal.pone.0230550
-
Younes, J.A., Van der Mei, H.C., Van den Heuvel, E., Busscher, H.J. and Reid, G., 2012. Adhesion forces and coaggregation between vaginal staphylococci and lactobacilli. PLoS ONE 7: e36917. https://doi.org/10.1371/journal.pone.0036917
Supplementary Materials
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Lactobacillus crispatus DSM25988 as novel bioactive agent to co-aggregate Streptococcus pyogenes and to exclude it by binding to human cells
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Streptococcus pyogenes, a group A streptococcus, is the major bacterial pathogen responsible for acute bacterial infection of the human oropharynx and the causative agent of scarlet fever. Estimates of the global burden of S. pyogenes related diseases revealed 616 million cases of pharyngitis, and at least 517,000 deaths due to severe invasive diseases and sequelae. Here we describe Lactobacillus crispatus DSM25988 that was identified among hundreds of Lactobacillus strains (referring to all organisms that were classified as Lactobacillaceae until 2020) showing ability to prevent adhesion of S. pyogenes to Detroit 562 cells, and to exhibit a masking and co-aggregating effect on S. pyogenes in vitro. L. crispatus DSM25988 also inhibits invasion of cultured human epithelial pharyngeal cells by S. pyogenes. Competitive binding to fibronectin might be involved in the inhibition process. Antiviral activity of the L. crispatus DSM25988 cells were identified in an in vitro cell model demonstrating that L. crispatus effectively excludes viruses from epithelial cells using SARS-CoV2 proteins as a model. This finding points to the potential of DSM25988 to protect cells from virus infection. Biological activity is retained in heat treated cells. The heat-treated Lactobacillus strain was further developed into functional throat lozenges, wherein its biological activity is stably maintained in the formulation. Lozenges containing L. crispatus DSM25988 underwent testing in an uncontrolled, prospective user study in 44 subjects with symptoms of sore throat for a period of up to 14 days. The study data shows promising safety and efficacy of the medical device when used against symptoms of sore throat like scratchy feeling, hoarse voice and swallowing pain.
| Insgesamt | Letzte 365 Tage | In den letzten 30 Tagen | |
|---|---|---|---|
| Aufrufe von Kurzbeschreibungen | 0 | 0 | 0 |
| Gesamttextansichten | 552 | 207 | 16 |
| PDF-Downloads | 419 | 155 | 11 |
