Guidelines for best practice in placebo-controlled experimental studies on probiotics in rodent animal models
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In the absence of established best practice standards in the probiotic field for reducing the risk of bacterial transfer between experimental groups, we developed protocols and methods to ensure the highest quality and interpretability of results from animal studies, even when performed in non-conventional animal care facilities. We describe easily implementable methods for reducing cross-contamination during animal housing, behavioural testing, and euthanasia, along with highlighting protocols for contamination detection in experimental subjects and laboratory areas using qPCR. In light of the high cross-contamination risks between animals during experiments involving probiotics, constant vigilance in animal care and research protocols is critical to ensure valid and reliable research findings.
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Barnett, J.A. and Gibson, D.L., 2019. H2Oh No! The importance of reporting your water source in your in vivo microbiome studies. Gut Microbes 10: 261-269. https://doi.org/10.1080/19490976.2018.1539599
-
Bustin, S.A., Benes, V., Garson, J.A., Hellemans, J., Huggett, J., Kubista, M., Mueller, R., Nolan, T., Pfaffl, M.W., Shipley, G.L. and Vandesompele, J., 2009. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clinical Chemistry 55: 611-622. https://doi.org/10.1373/clinchem.2008.112797
-
Collins, S.M., Denou, E., Verdu, E.F. and Bercik, P., 2009. The putative role of the intestinal microbiota in the irritable bowel syndrome. Digestive and Liver Disease 41: 850-853. https://doi.org/10.1016/j.dld.2009.07.023
-
Costeloe, K., Bowler, U., Brocklehurst, P., Hardy, P., Heal, P., Juszczak, E., King, A., Panton, N., Stacey, F., Whiley, A., Wilks, M. and Millar, M.R., 2016. A randomised controlled trial of the probiotic Bifidobacterium breve BBG-001 in preterm babies to prevent sepsis, necrotising enterocolitis and death: the Probiotics in Preterm infantS (PiPS) trial. Health Technology Assessment 20: 66. https://doi.org/10.3310/hta20660
-
De Waard, R., Snel, J., Bokken, G.C.A.M., Tan, P.S.T., Schut, F. and Huis in ‘t Veld, J.H.J., 2002. Comparison of faecal Lactobacillus populations in experimental animals from different breeding facilities and possible consequences for probiotic studies. Letters in Applied Microbiology 34: 105-109. https://doi.org/10.1046/j.1472-765x.2002.01051.x
-
Deshpande, G.C., Rao, S.C., Keil, A.D. and Patole, S.K., 2011. Evidence-based guidelines for use of probiotics in preterm neonates. BMC Medicine 9: 92. https://doi.org/10.1186/1741-7015-9-92
-
Foster, L., Tompkins, T. and Dahl, W., 2011. A comprehensive post-market review of studies on a probiotic product containing Lactobacillus helveticus R0052 and Lactobacillus rhamnosus R0011. Beneficial Microbes 2: 319-334. https://doi.org/10.3920/BM2011.0032
-
Hartman, A.L., Lough, D.M., Barupal, D.K., Fiehn, O., Fishbein, T., Zasloff, M. and Eisen, J.A., 2009. Human gut microbiome adopts an alternative state following small bowel transplantation. Proceedings of the National Academy of Sciences of the USA 106: 17187-17192. https://doi.org/10.1073/pnas.0904847106
-
Hill, C., Guarner, F., Reid, G., Gibson, G.R., Merenstein, D.J., Pot, B., Morelli, L., Canani, R.B., Flint, H.J., Salminen, S., Calder, P.C. and Sanders, M.E., 2014. Expert consensus document: the International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nature Reviews Gastroenterology and Hepatology 11: 506-514. https://doi.org/10.1038/nrgastro.2014.66
-
Hussain, S.S. and Bloom, S.R., 2013. The regulation of food intake by the gut-brain axis: implications for obesity. International Journal of Obesity 37: 625-633. https://doi.org/10.1038/ijo.2012.93
-
Luna, R.A. and Foster, J.A., 2015. Gut brain axis: diet microbiota interactions and implications for modulation of anxiety and depression. Current Opinion in Biotechnology 32: 35-41. https://doi.org/10.1016/j.copbio.2014.10.007
-
MacPherson, C.W., Mathieu, O., Tremblay, J., Champagne, J., Nantel, A., Girard, S.A. and Tompkins, T.A., 2018. Gut bacterial microbiota and its resistome rapidly recover to basal state levels after short-term amoxicillin-clavulanic acid treatment in healthy adults. Scientific Reports 8: 11192. https://doi.org/10.1038/s41598-018-29229-5
-
Patole, S., Keil, A.D., Chang, A., Nathan, E., Doherty, D., Simmer, K., Esvaran, M. and Conway, P., 2014. Effect of Bifidobacterium breve M-16V supplementation on fecal bifidobacteria in preterm neonates-a randomised double-blind placebo controlled trial. PLoS ONE 9: e89511. https://doi.org/10.1371/journal.pone.0089511
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Preidis, G.A., Saulnier, D.M., Blutt, S.E., Mistretta, T.A., Riehle, K.P., Major, A.M., Venable, S.F., Finegold, M.J., Petrosino, J.F., Conner, M.E. and Versalovic, J., 2012. Probiotics stimulate enterocyte migration and microbial diversity in the neonatal mouse intestine. FASEB Journal 26: 1960-1969. https://doi.org/10.1096/fj.10-177980
-
Robertson, S.J., Lemire, P., Maughan, H., Goethel, A., Turpin, W., Bedrani, L., Guttman, D.S., Croitoru, K., Girardin, S.E. and Philpott, D.J., 2019. Comparison of co-housing and littermate methods for microbiota standardization in mouse models. Cell Reports 27: 1910-1919. https://doi.org/10.1016/j.celrep.2019.04.023
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Sanders, M.E., 2009. How do we know when something called ‘probiotic’ is really a probiotic? A guideline for consumers and health care professionals. Functional Food Reviews 1: 3-12.
'How do we know when something called ‘probiotic’ is really a probiotic? ' () 1 Functional Food Reviews : 3 -12 .
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Tillmann, S. and Wegener, G., 2018. Syringe-feeding as a novel delivery method for accurate individual dosing of probiotics in rats. Beneficial Microbes 9: 311-315. https://doi.org/10.3920/BM2017.0127
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Tillmann, S. and Wegener, G., 2019. Probiotics reduce risk-taking behavior in the elevated plus maze in the flinders sensitive line rat model of depression. Behavioural Brain Research 359: 755-762. https://doi.org/10.1016/j.bbr.2018.08.025
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Guidelines for best practice in placebo-controlled experimental studies on probiotics in rodent animal models
In: Beneficial MicrobesSearch for other papers by E.M. Myles in
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PubMed
Search for other papers by M.E. O’Leary in
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Search for other papers by I.D. Romkey in
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Search for other papers by A. Piano in
Current site
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PubMed
Search for other papers by V. de Carvalho in
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PubMed
Search for other papers by T.A. Tompkins in
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PubMed
Search for other papers by T.S. Perrot in
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PubMed
In the absence of established best practice standards in the probiotic field for reducing the risk of bacterial transfer between experimental groups, we developed protocols and methods to ensure the highest quality and interpretability of results from animal studies, even when performed in non-conventional animal care facilities. We describe easily implementable methods for reducing cross-contamination during animal housing, behavioural testing, and euthanasia, along with highlighting protocols for contamination detection in experimental subjects and laboratory areas using qPCR. In light of the high cross-contamination risks between animals during experiments involving probiotics, constant vigilance in animal care and research protocols is critical to ensure valid and reliable research findings.
| All Time | Past 365 days | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 0 | 0 | 0 |
| Full Text Views | 286 | 95 | 16 |
| PDF Views & Downloads | 213 | 66 | 8 |
